A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\PBac{RB}CG8920e01132 Insertion

General Information
Symbol Dmel\PBac{RB}CG8920e01132 Species D. melanogaster
Name FlyBase ID FBti0046884
Feature type transposable_element_insertion_site
Description
Inserted element PBac{RB} Expression data
Affected gene(s) CG8920 Viability / fertility
Causes allele(s) CG8920e01132 Stock availability 1 publicly available
LINE ID e01132
Genomic Location
Chromosomal location 2R ( 56F16 ) Sequence location 2R:16,208,284..16,208,284 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0038985 FBti0144087 FBti0057291 FBti0027109 FBti0111392 FBti0127606 FBti0017754 FBti0035402 FBti0113480 FBti0033689 FBti0059265 FBti0108456 FBti0034245 FBti0039798 FBti0112377 FBti0108555 FBti0108554 FBti0110198 FBti0109661 FBti0111249 FBti0102175 FBti0102386 FBti0033745 FBti0036254 FBti0035391 FBti0104111 FBti0026420 FBti0106299 FBti0027159 FBti0104102 FBti0099775 FBti0046884 FBti0066478 FBti0112972 FBti0026150 FBti0076013 FBti0112935 FBti0039704 FBti0023298 FBti0111975 FBti0106014 FBti0043777 FBti0112663 FBti0007745 FBti0108847 FBti0108493 FBti0007720 FBti0027031 FBti0108076
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the Tni\piggyBac-based construct PBac{RB}. The PBac{RB} construct carries the w[+mC] mini-white marker, a single long (199-bp) Scer\FRT site, and a "splice trap," a splice acceptor site derived from a D. melanogaster gene. The FRT site allows Scer\FLP-mediated recombination between other FRT-containing elements, and thus can be used to generate molecularly defined deletions.
PBac{RB} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Thibault et al., 2004, Bellen et al., 2011)
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
2R
(Thibault, 2004)
Sequence Location
2R:16,208,284..16,208,284 [+]
(Gene Disruption Project members and Exelixis, 2005)
Orientation
+
(Thibault, 2004)
Cytological location
(computed by FlyBase)
56F16 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
56F16 (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
Comments concerning
location
hide Sequence Data
Flanking sequence
CZ478951
(Gene Disruption Project members, 2001-)
hide Inserted Element
Construct PBac{RB}
Location-dependent
role
deletion generation component
(Thibault et al., 2004)
carries FRT site(s)
(Thibault et al., 2004)
Size 5.971Kb
Associated alleles
Rbp1SA.RB
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
CG8920
(Gene Disruption Project members, 2001-)
hide Alleles and Phenotypes
Causes alleles
CG8920e01132
(Gene Disruption Project members and Exelixis, 2005)
Lethality
References
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
e01132
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Harvard
-
PBac{RB}CG8920e01132
hide Linkouts
hide Comments
Location 2R:15835641-15835642 refined by FlyBase alignment of dbGSS accession CZ478951 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
PBac{RB}CG8920e01132
(FlyBase, 2005, FlyBase, 1992-, )
PBac{RB}e01132
(Thibault, 2004, FlyBase, 1992-)
Secondary FlyBase IDs
hide References ( 9 )
Research paper
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Thibault et al., 2004, Nat. Genet. 36(3): 283--287
A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material
Thibault, 2004, Nature Genetics 36(3):
Supplementary Table 3. [FBrf0174228]
Personal communication to FlyBase
Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. (Computer file)
Genomic mapping of Exelixis insertion collection. (Computer file) [FBrf0184340]
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
FlyBase analysis
FlyBase Curators, 2013, Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}.
Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. [FBrf0221061]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]