A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\PBac{RB}veloe01260 Insertion

General Information
Symbol Dmel\PBac{RB}veloe01260 Species D. melanogaster
Name FlyBase ID FBti0046975
Feature type transposable_element_insertion_site
Description
Inserted element PBac{RB} Expression data
Affected gene(s) velo Viability / fertility
Causes allele(s) veloe01260 Stock availability 1 publicly available
LINE ID e01260
Genomic Location
Chromosomal location 3L ( 65C3 ) Sequence location 3L:6,740,123..6,740,123 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0035984 FBti0109323 FBti0102919 FBti0021235 FBti0070357 FBti0102607 FBti0109896 FBti0043579 FBti0056792 FBti0046975 FBti0053424 FBti0103790 FBti0111168 FBti0100349 FBti0102814 FBti0021911 FBti0112583 FBti0072553 FBti0115524 FBti0050144 FBti0115586
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the Tni\piggyBac-based construct PBac{RB}. The PBac{RB} construct carries the w[+mC] mini-white marker, a single long (199-bp) Scer\FRT site, and a "splice trap," a splice acceptor site derived from a D. melanogaster gene. The FRT site allows Scer\FLP-mediated recombination between other FRT-containing elements, and thus can be used to generate molecularly defined deletions.
PBac{RB} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Thibault et al., 2004, Bellen et al., 2011)
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
3L
(Thibault, 2004)
Sequence Location
3L:6,740,123..6,740,123 [+]
(Gene Disruption Project members and Exelixis, 2005)
Orientation
+
(Thibault, 2004)
Cytological location
(computed by FlyBase)
65C3 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
65C3 (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
Comments concerning
location
hide Sequence Data
Flanking sequence
CZ479066
(Gene Disruption Project members, 2001-)
hide Inserted Element
Construct PBac{RB}
Location-dependent
role
deletion generation component
(Thibault et al., 2004)
carries FRT site(s)
(Thibault et al., 2004)
Size 5.971Kb
Associated alleles
Rbp1SA.RB
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
velo
(Gene Disruption Project members, 2001-, Berdnik et al., 2012)
hide Alleles and Phenotypes
Causes alleles
veloe01260
(Gene Disruption Project members and Exelixis, 2005, Berdnik et al., 2012)
Lethality
References
lethal
(Berdnik et al., 2012)
Sterility
References
hide Phenotype Manifest In
adult antennal lobe projection neuron DL1 adPN | somatic clone
(Berdnik et al., 2012)
antennal lobe glomerulus DL1 | somatic clone
(Berdnik et al., 2012)
hide Detailed Description
Statement
Reference
velo[e01260] mutants display projection neuron dendrite targeting defects. velo[e01260] mutant neuroblast clones exhibit several defects. First, the number of neurons is reduced from an average of 35 ad projection neurons in wild-type to 5 neurons in velo[e01260] mutant clones. Second, the overall dendritic mass is reduced and disorganised Third, dendrites aberrantly innervate the wrong glomeruli within the antennal lobe, or ectopically project outside the antennal lobe. velo[e01260] mutant DL1 projection neurons exhibit defects in axon morphology. Axons extend as wild-type and always reach the end of the lateral horn, but only form 0-2 collaterals in the mushroom body calyx, and often have a missing or shorter dorsal branch in the lateral horn. These axonal phenotypes are independent of the phenotypic class of dendrite mistargeting. In velo[e01260] mutant neuroblast clones projection neuron cell numbers are drastically reduced from ~35 neurons in wild-type to an average of 5.5 neurons.
(Berdnik et al., 2012)
Expression of CG12717[Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[GH146] suppresses the DL1 projection neuron mistargeting phenotype found in velo[e01260] mutants. However, velo[e01260] mutant axon phenotypes cannot be reverted by CG12717[Scer\UAS.T:Ivir\HA1] expression. Expression of Ulp1[Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[GH146] suppresses the DL1 projection neuron mistargeting phenotype found in velo[e01260] mutants. However, velo[e01260] mutant axon phenotypes cannot be reverted by CG12717[Scer\UAS.T:Ivir\HA1] expression. The number of velo[e01260] mutant neuroblast clone projection neuron cells is increased upon Ulp1[Scer\UAS.T:Ivir\HA1] or CG12717[Scer\UAS.T:Ivir\HA1] expression (under the control of Scer\GAL4[GH146]). However, the overall number is still lower than in wild-type.
(Berdnik et al., 2012)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
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FlyView (LinkOut)
hide Data on Genetic Line
Line ID
e01260
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Harvard
-
PBac{RB}veloe01260
hide Linkouts
hide Comments
Location 3L:6720969-6720970 refined by FlyBase alignment of dbGSS accession CZ479066 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
e01260
(Berdnik et al., 2012)
PBac{RB}CG10107e01260
(FlyBase, 2005, FlyBase, 1992-)
PBac{RB}e01260
(Thibault, 2004, FlyBase, 1992-)
PBac{RB}veloe01260
 
Secondary FlyBase IDs
hide References ( 10 )
Research paper
Berdnik et al., 2012, J. Neurosci. 32(24): 8331--8340
The SUMO Protease Verloren Regulates Dendrite and Axon Targeting in Olfactory Projection Neurons. [FBrf0218602]
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Thibault et al., 2004, Nat. Genet. 36(3): 283--287
A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material
Thibault, 2004, Nature Genetics 36(3):
Supplementary Table 3. [FBrf0174228]
Personal communication to FlyBase
Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. (Computer file)
Genomic mapping of Exelixis insertion collection. (Computer file) [FBrf0184340]
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
FlyBase analysis
FlyBase Curators, 2013, Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}.
Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. [FBrf0221061]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]