Dmel\PBac{RB}Snx16^e01600 Insertion

General Information
Symbol	Dmel\PBac{RB}Snx16^e01600	Species	D. melanogaster
Name		FlyBase ID	FBti0047197
Feature type	transposable_element_insertion_site
Description
Inserted element	PBac{RB}	Expression data
Affected gene(s)	Snx16	Viability / fertility
Causes allele(s)	Snx16^e01600	Stock availability	1 publicly available
LINE ID	e01600
Genomic Location
Chromosomal location	2R ( 54E2 )	Sequence location	2R:13,614,126..13,614,126 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_PBac{RB}.Exel.e A set of transgenic insertion stocks derived by TE mobilization using the Tni\piggyBac-based construct PBac{RB}. The PBac{RB} construct carries the w[+mC] mini-white marker, a single long (199-bp) Scer\FRT site, and a "splice trap," a splice acceptor site derived from a D. melanogaster gene. The FRT site allows Scer\FLP-mediated recombination between other FRT-containing elements, and thus can be used to generate molecularly defined deletions. PBac{RB} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank. (Thibault et al., 2004, Bellen et al., 2011)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)	2R (Thibault, 2004)
Sequence Location	2R:13,614,126..13,614,126 [+] (Gene Disruption Project members and Exelixis, 2005)
Orientation	+ (Thibault, 2004)
Cytological location (computed by FlyBase)	54E2 ( inferred by FlyBase from sequence location )
Cytological location (reported)	54E2 (reported as inferred from sequence location) (Gene Disruption Project members, 2001-)
Comments concerning location
Sequence Data
Flanking sequence	CZ479416 (Gene Disruption Project members, 2001-)
Inserted Element
Construct	PBac{RB}
Location-dependent role	deletion generation component (Thibault et al., 2004) carries FRT site(s) (Thibault et al., 2004)
Size	5.971Kb
Associated alleles	Rbp1^SA.RB w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Snx16 (Gene Disruption Project members, 2001-, Rodal et al., 2011)
Alleles and Phenotypes
Causes alleles	Snx16^e01600 (Gene Disruption Project members and Exelixis, 2005, Rodal et al., 2011)

Lethality References
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference Average bouton number and average satellite bouton number at the neuromuscular junction is not significantly different from wild type in Snx16[e01600]/Snx16[Δ1], Snx16[e01600]/Snx16[Δ2] and Snx16[e01600]/Snx16[Δpre] animals. (Rodal et al., 2011) The increase in average bouton number and satellite bouton number at the neuromuscular junction which is seen in nwk[2] larvae is suppressed if they are also carrying either Snx16[Δ1]/Snx16[e01600] or Snx16[Δ2]/Snx16[e01600]. Average bouton number and average satellite bouton number at the neuromuscular junction is significantly increased compared to wild type in Snx16[e01600] Hrs[D28]/Snx16[Δ1] Df(2L)Exel6277 double mutants. (Rodal et al., 2011)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	e01600 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Harvard	- PBac{RB}Snx16^e01600
Linkouts
Comments
	Location 2R:13241483-13241484 refined by FlyBase alignment of dbGSS accession CZ479416 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	PBac{RB}CG6410^e01600 (FlyBase, 2005, FlyBase, 1992-) PBac{RB}e01600 (Thibault, 2004, FlyBase, 1992-) PBac{RB}Snx16^e01600 PBac^RBCG6410^e01600 (Rodal et al., 2011)
Secondary FlyBase IDs

References ( 10 )
Research paper	Bellen et al., 2011, Genetics 188(3): 731--743 The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229] Rodal et al., 2011, J. Cell Biol. 193(1): 201--217 A presynaptic endosomal trafficking pathway controls synaptic growth signaling. [FBrf0213353] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Thibault et al., 2004, Nat. Genet. 36(3): 283--287 A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material	Thibault, 2004, Nature Genetics 36(3): Supplementary Table 3. [FBrf0174228]
Personal communication to FlyBase	Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. (Computer file) Genomic mapping of Exelixis insertion collection. (Computer file) [FBrf0184340] Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177]
FlyBase analysis	FlyBase Curators, 2013, Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. [FBrf0221061] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\PBac{RB}Snx16e01600 Insertion

Dmel\PBac{RB}Snx16^e01600 Insertion