A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\PBac{RB}ihoge02142 Insertion

General Information
Symbol Dmel\PBac{RB}ihoge02142 Species D. melanogaster
Name FlyBase ID FBti0047529
Feature type transposable_element_insertion_site
Description
Inserted element PBac{RB} Expression data
Affected gene(s) ihog Viability / fertility
Causes allele(s) ihoge02142 Stock availability 1 publicly available
LINE ID e02142
Genomic Location
Chromosomal location 2L ( 27C6 ) Sequence location 2L:6,948,694..6,948,694 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0021650 FBti0055741 FBti0124619 FBti0044838 FBti0047768 FBti0103092 FBti0106620 FBti0024935 FBti0039824 FBti0124620_2 FBti0124620_1 FBti0047529 FBti0116021 FBti0036861 FBti0042470 FBti0034728 FBti0075877 FBti0025877 FBti0052421 FBti0126502 FBti0051670 FBti0025749 FBti0039614
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the Tni\piggyBac-based construct PBac{RB}. The PBac{RB} construct carries the w[+mC] mini-white marker, a single long (199-bp) Scer\FRT site, and a "splice trap," a splice acceptor site derived from a D. melanogaster gene. The FRT site allows Scer\FLP-mediated recombination between other FRT-containing elements, and thus can be used to generate molecularly defined deletions.
PBac{RB} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Thibault et al., 2004, Bellen et al., 2011)
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Description
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What does this section not display?
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
2L
(Thibault, 2004)
Sequence Location
2L:6,948,694..6,948,694 [+]
(Gene Disruption Project members and Exelixis, 2005)
Orientation
+
(Thibault, 2004)
Cytological location
(computed by FlyBase)
27C6 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
27C6 (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
Comments concerning
location
hide Sequence Data
Flanking sequence
CZ479968
(Gene Disruption Project members, 2001-)
hide Inserted Element
Construct PBac{RB}
Location-dependent
role
deletion generation component
(Thibault et al., 2004)
carries FRT site(s)
(Thibault et al., 2004)
Size 5.971Kb
Associated alleles
Rbp1SA.RB
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
ihog
(Gene Disruption Project members, 2001-)
hide Alleles and Phenotypes
Causes alleles
ihoge02142
(Gene Disruption Project members and Exelixis, 2005, Yao et al., 2006)
Lethality
References
viable
(Yao et al., 2006)
Sterility
References
fertile
(Yao et al., 2006)
hide Phenotype Manifest In
denticle belt | embryonic stage
(Yao et al., 2006)
hide Detailed Description
Statement
Reference
ihog[e02142] mutants exhibit wild-type follicle stem cell proliferation and follicle production.
(Hartman et al., 2010)
The penetrance and severity of the follicle cell phenotypes of homozygous boi[e01708] flies are not affected by ihog[e02142]/+.
(Hartman et al., 2010)
Paternally-provided iHoge02142 lessens the severity of the denticle belt fusions seen in embryos from either iHogKG or iHogEY maternal germ-line clones.
(Yao et al., 2006)
iHoge02142; dlpA187 zygotic mutants show denticle belt fusions.
(Yao et al., 2006)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
e02142
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Harvard
-
PBac{RB}ihoge02142
hide Linkouts
hide Comments
Location 2L:6948693-6948694 refined by FlyBase alignment of dbGSS accession CZ479968 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
PBac{RB}e02142
(Thibault, 2004, FlyBase, 1992-)
PBac{RB}iHoge02142
(FlyBase, 1992-, FlyBase, 2005)
PBac{RB}ihoge02142
 
pBac{RB}iHoge02142
(Camp et al., 2010)
Secondary FlyBase IDs
hide References ( 12 )
Research paper
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Camp et al., 2010, Neural Dev. 5: 28
Ihog and Boi are essential for Hedgehog signaling in Drosophila. [FBrf0212294]
Hartman et al., 2010, J. Cell Biol. 191(5): 943--952
Drosophila Boi limits Hedgehog levels to suppress follicle stem cell proliferation. [FBrf0212398]
Yao et al., 2006, Cell 125(2): 343--357
The ihog cell-surface proteins bind Hedgehog and mediate pathway activation. [FBrf0189929]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Thibault et al., 2004, Nat. Genet. 36(3): 283--287
A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material
Thibault, 2004, Nature Genetics 36(3):
Supplementary Table 3. [FBrf0174228]
Personal communication to FlyBase
Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. (Computer file)
Genomic mapping of Exelixis insertion collection. (Computer file) [FBrf0184340]
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
FlyBase analysis
FlyBase Curators, 2013, Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}.
Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. [FBrf0221061]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]