A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\PBac{RB}Jheh2e02983 Insertion

General Information
Symbol Dmel\PBac{RB}Jheh2e02983 Species D. melanogaster
Name FlyBase ID FBti0048023
Feature type transposable_element_insertion_site
Description
Inserted element PBac{RB} Expression data
Affected gene(s) Jheh2 Viability / fertility
Causes allele(s) Jheh2e02983 Stock availability 1 publicly available
LINE ID e02983
Genomic Location
Chromosomal location 2R ( 55F8 ) Sequence location 2R:14,744,937..14,744,938 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBti0018880 FBti0126640 FBti0059278 FBti0076126 FBti0106689 FBti0109335 FBti0109317 FBti0026205 FBti0052415 FBti0037595 FBti0107408 FBti0039444 FBti0011193 FBti0109935 FBti0125288_2 FBti0125288_1 FBti0046860 FBti0042910 FBti0048023 FBti0108752 FBti0020919 FBti0034974 FBti0036076 FBti0105290 FBti0033916 FBti0011385 FBti0103531 FBti0107087 FBti0102760 FBti0035519 FBti0054558 FBti0034353 FBti0035517 FBti0104644 FBti0055242 FBti0125289_2 FBti0125289_1 FBti0050468 FBti0039591 FBti0036519 FBti0034256 FBti0028267 FBti0056634 FBti0069958 FBti0024707 FBti0114272
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the Tni\piggyBac-based construct PBac{RB}. The PBac{RB} construct carries the w[+mC] mini-white marker, a single long (199-bp) Scer\FRT site, and a "splice trap," a splice acceptor site derived from a D. melanogaster gene. The FRT site allows Scer\FLP-mediated recombination between other FRT-containing elements, and thus can be used to generate molecularly defined deletions.
PBac{RB} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Thibault et al., 2004, Bellen et al., 2011)
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
2R
(Thibault, 2004)
Sequence Location
2R:14,744,937..14,744,938 [-]
(Gene Disruption Project members and Exelixis, 2005)
Orientation
-
(Thibault, 2004)
Cytological location
(computed by FlyBase)
55F8 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
55F8 (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
Comments concerning
location
hide Sequence Data
Flanking sequence
CZ480750
(Gene Disruption Project members, 2001-)
CZ480751
(Gene Disruption Project members, 2001-)
hide Inserted Element
Construct PBac{RB}
Location-dependent
role
deletion generation component
(Thibault et al., 2004)
carries FRT site(s)
(Thibault et al., 2004)
Size 5.971Kb
Associated alleles
Rbp1SA.RB
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Jheh2
(Gene Disruption Project members, 2001-)
hide Alleles and Phenotypes
Causes alleles
Jheh2e02983
(Gene Disruption Project members and Exelixis, 2005)
Lethality
References
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
e02983
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Harvard
-
PBac{RB}Jheh2e02983
hide Linkouts
hide Comments
Location 2R:14372294-14372297 confirmed by FlyBase alignment of dbGSS accessions CZ480751 and CZ480750 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
PBac{RB}e02983
(Thibault, 2004, FlyBase, 1992-)
PBac{RB}Jheh2e02983
(FlyBase, 2005, FlyBase, 1992-, )
Secondary FlyBase IDs
hide References ( 9 )
Research paper
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Thibault et al., 2004, Nat. Genet. 36(3): 283--287
A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material
Thibault, 2004, Nature Genetics 36(3):
Supplementary Table 3. [FBrf0174228]
Personal communication to FlyBase
Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. (Computer file)
Genomic mapping of Exelixis insertion collection. (Computer file) [FBrf0184340]
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
FlyBase analysis
FlyBase Curators, 2013, Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}.
Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. [FBrf0221061]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]