A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\PBac{RB}CG31475e03702 Insertion

General Information
Symbol Dmel\PBac{RB}CG31475e03702 Species D. melanogaster
Name FlyBase ID FBti0048444
Feature type transposable_element_insertion_site
Description
Inserted element PBac{RB} Expression data
Affected gene(s) CG31475 Viability / fertility
Causes allele(s) CG31475e03702 Stock availability 1 publicly available
LINE ID e03702
Genomic Location
Chromosomal location 3R ( 91F11 ) Sequence location 3R:15,006,623..15,006,623 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0040993 FBti0044009 FBti0036594 FBti0024839 FBti0072821 FBti0016488 FBti0068092 FBti0050655 FBti0102826 FBti0100906 FBti0007868 FBti0111802 FBti0108271 FBti0106093 FBti0111549 FBti0103624 FBti0016553 FBti0016561 FBti0113306 FBti0040197 FBti0109341 FBti0104156 FBti0104537 FBti0105767 FBti0102316 FBti0106759 FBti0103035 FBti0059608 FBti0007817 FBti0109680 FBti0007873 FBti0100008 FBti0104363 FBti0106224 FBti0011574 FBti0112547 FBti0112096 FBti0058953 FBti0106728 FBti0036263 FBti0028872 FBti0109933 FBti0113316 FBti0055926 FBti0111289 FBti0110417 FBti0059423 FBti0011420 FBti0007846 FBti0021677 FBti0104846 FBti0028166 FBti0105979 FBti0007922 FBti0011365 FBti0104152 FBti0104295 FBti0033949 FBti0104381 FBti0030009 FBti0048444 FBti0029479 FBti0066716 FBti0077703
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the Tni\piggyBac-based construct PBac{RB}. The PBac{RB} construct carries the w[+mC] mini-white marker, a single long (199-bp) Scer\FRT site, and a "splice trap," a splice acceptor site derived from a D. melanogaster gene. The FRT site allows Scer\FLP-mediated recombination between other FRT-containing elements, and thus can be used to generate molecularly defined deletions.
PBac{RB} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Thibault et al., 2004, Bellen et al., 2011)
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Description
What does this section display?
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
3R
(Thibault, 2004)
Sequence Location
3R:15,006,623..15,006,623 [+]
(Gene Disruption Project members and Exelixis, 2005)
Orientation
+
(Thibault, 2004)
Cytological location
(computed by FlyBase)
91F11 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
91F11 (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
Comments concerning
location
hide Sequence Data
Flanking sequence
CZ481458
(Gene Disruption Project members, 2001-)
hide Inserted Element
Construct PBac{RB}
Location-dependent
role
deletion generation component
(Thibault et al., 2004)
carries FRT site(s)
(Thibault et al., 2004)
Size 5.971Kb
Associated alleles
Rbp1SA.RB
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
CG31475
(Gene Disruption Project members, 2001-)
hide Alleles and Phenotypes
Causes alleles
CG31475e03702
(Gene Disruption Project members and Exelixis, 2005)
Lethality
References
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
e03702
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Harvard
-
PBac{RB}CG31475e03702
hide Linkouts
hide Comments
Location 3R:15006622-15006623 refined by FlyBase alignment of dbGSS accession CZ481458 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
PBac{RB}CG31475e03702
(FlyBase, 2005, FlyBase, 1992-, )
PBac{RB}e03702
(Thibault, 2004, FlyBase, 1992-)
Secondary FlyBase IDs
hide References ( 9 )
Research paper
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Thibault et al., 2004, Nat. Genet. 36(3): 283--287
A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material
Thibault, 2004, Nature Genetics 36(3):
Supplementary Table 3. [FBrf0174228]
Personal communication to FlyBase
Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. (Computer file)
Genomic mapping of Exelixis insertion collection. (Computer file) [FBrf0184340]
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
FlyBase analysis
FlyBase Curators, 2013, Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}.
Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. [FBrf0221061]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]