A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\PBac{RB}CG43736e04592 Insertion

General Information
Symbol Dmel\PBac{RB}CG43736e04592 Species D. melanogaster
Name FlyBase ID FBti0048938
Feature type transposable_element_insertion_site
Description
Inserted element PBac{RB} Expression data
Affected gene(s) CG43736 Viability / fertility
Causes allele(s) CG43736e04592 Stock availability 1 publicly available
LINE ID e04592
Genomic Location
Chromosomal location X ( 6E2 ) Sequence location X:6,767,145..6,767,145 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0061605 FBti0035597 FBti0048815 FBti0047677 FBti0066156 FBti0020895 FBti0068094 FBti0070781 FBti0049796 FBti0068885 FBti0070707 FBti0109496 FBti0059036 FBti0071932 FBti0007368 FBti0046555 FBti0066658 FBti0011879 FBti0055407 FBti0039910 FBti0054748 FBti0018153 FBti0077061 FBti0071435 FBti0043915 FBti0065639 FBti0066531 FBti0048938 FBti0049143 FBti0065782 FBti0037304 FBti0024933 FBti0071275
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the Tni\piggyBac-based construct PBac{RB}. The PBac{RB} construct carries the w[+mC] mini-white marker, a single long (199-bp) Scer\FRT site, and a "splice trap," a splice acceptor site derived from a D. melanogaster gene. The FRT site allows Scer\FLP-mediated recombination between other FRT-containing elements, and thus can be used to generate molecularly defined deletions.
PBac{RB} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Thibault et al., 2004, Bellen et al., 2011)
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
X
(Thibault, 2004)
Sequence Location
X:6,767,145..6,767,145 [+]
(Gene Disruption Project members and Exelixis, 2005)
Orientation
+
(Thibault, 2004)
Cytological location
(computed by FlyBase)
6E2 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
6E2 (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
Comments concerning
location
hide Sequence Data
Flanking sequence
CZ482348
(Gene Disruption Project members, 2001-)
CZ482349
(Gene Disruption Project members, 2001-)
hide Inserted Element
Construct PBac{RB}
Location-dependent
role
deletion generation component
(Thibault et al., 2004)
carries FRT site(s)
(Thibault et al., 2004)
Size 5.971Kb
Associated alleles
Rbp1SA.RB
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
CG43736
(FlyBase Curators, 2008)
hide Alleles and Phenotypes
Causes alleles
CG43736e04592
(FlyBase Curators, 2008)
Lethality
References
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
e04592
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Harvard
-
PBac{RB}CG43736e04592
hide Linkouts
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
PBac{RB}CG33691e04592
 
PBac{RB}CG43736e04592
 
PBac{RB}e04592
(Thibault, 2004, FlyBase, 1992-)
Secondary FlyBase IDs
hide References ( 9 )
Research paper
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Thibault et al., 2004, Nat. Genet. 36(3): 283--287
A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material
Thibault, 2004, Nature Genetics 36(3):
Supplementary Table 3. [FBrf0174228]
Personal communication to FlyBase
Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. (Computer file)
Genomic mapping of Exelixis insertion collection. (Computer file) [FBrf0184340]
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
FlyBase analysis
FlyBase Curators, 2013, Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}.
Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. [FBrf0221061]
FlyBase Curators, 2008, Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations.
Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. [FBrf0202955]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]