Dmel\PBac{WH}DopR^f02676 Insertion

General Information
Symbol	Dmel\PBac{WH}DopR^f02676	Species	D. melanogaster
Name		FlyBase ID	FBti0050754
Feature type	transposable_element_insertion_site
Description
Inserted element	PBac{WH}	Expression data
Affected gene(s)	DopR	Viability / fertility
Causes allele(s)	DopR^f02676	Stock availability	1 publicly available
LINE ID	f02676
Genomic Location
Chromosomal location	3R ( 88A11 )	Sequence location	3R:10,032,116..10,032,116 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_PBac{WH}.Exel.f A set of transgenic insertion stocks derived by TE mobilization using the Tni\piggyBac-based construct PBac{WH}. The PBac{WH} construct carries the w[+mC] mini-white marker, a single long (199-bp) Scer\FRT site, and a terminal Scer\UAS site for Scer\GAL4-driven misexpression of adjacent genes. The FRT site allows Scer\FLP-mediated recombination between other FRT-containing elements, and thus can be used to generate molecularly defined deletions. PBac{WH} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank. (Thibault et al., 2004, Bellen et al., 2011)
Recent Updates
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)	3R (Thibault, 2004)
Sequence Location	3R:10,032,116..10,032,116 [+] (Gene Disruption Project members and Exelixis, 2005)
Orientation	+ (Thibault, 2004)
Cytological location (computed by FlyBase)	88A11 ( inferred by FlyBase from sequence location )
Cytological location (reported)	88A11 (reported as inferred from sequence location) (Gene Disruption Project members, 2001-)
Comments concerning location
Sequence Data
Flanking sequence	CZ485220 (Gene Disruption Project members, 2001-)
Inserted Element
Construct	PBac{WH}
Location-dependent role	deletion generation component (Thibault et al., 2004) mobile activating element (Thibault et al., 2004) carries FRT site(s) (Thibault et al., 2004)
Size	7.234Kb
Associated alleles	gypsy\su(Hw)BR³⁴⁸ Scer\UAS^{14x.hs.XP.Scer\FRT} Scer\UAS^14x.hs.XP TrlBR^cRa w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	DopR (Gene Disruption Project members, 2001-, Kim et al., 2007, Inagaki et al., 2012, Kong et al., 2010, Liu et al., 2012)
Alleles and Phenotypes
Causes alleles	DopR^f02676 (Gene Disruption Project members and Exelixis, 2005, Kim et al., 2007, Inagaki et al., 2012, Kong et al., 2010, Liu et al., 2012)

Lethality References
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference Expressing DopR[f02676] in Gr5a gustatory receptor neurons under the control of Scer\GAL4[Gr5a.8.5] rescues the defects in the sugar-sensitive proboscis extension reflex (PER) seen in DopR[f02676] mutant females. (Inagaki et al., 2012) DopR[f02676] mutant flies show a similar increase in sugar sensitivity in response to L-Dopa feeding to wild controls. (Inagaki et al., 2012) Expression of DopR (from the Scer\UAS sequences in the PBac{WH}DopR[f02676] insertion) under the control of either Scer\GAL4[104Y] or Scer\GAL4[c205] significantly restores the the reduced sleep caused by dopamine signaling in DopR[f02676]/DopR[f02676] flies fed L-dopa. This effect is not seen if expression is driven either by Scer\GAL4[ey-OK107] or by Scer\GAL4[c547]. (Liu et al., 2012) The decreased night-time sleep seen when wild-type flies are fed L-dopa is markedly suppressed in a DopR[f02676]/DopR[f02676] background. (Liu et al., 2012) The reduction in nighttime sleep seen in flies expressing TrpA1[Scer\UAS.cKa] under the control of Scer\GAL4[ple.PF] at 27[o]C is fully suppressed by DopR[f02676]/DopR[f02676]. (Liu et al., 2012) Flies of the genotype Scer\GAL4[5.30]/+; DopR[f02676]/DopR[f02676] show selectively restored expression of DopR. Those flies that had restored expression in patterns that include the R2/R4 class ellipsoid body neurons exhibit rescue of ethanol-induced hyperactivity. Restoring DopR expression in the R3 neurons of the ellipsoid body neurons (through Scer\GAL4[lilli-189Y]) results in a modest but statistically significant increase in ethanol-induced hyperactivity. However, expression with Scer\GAL4[Aph-4-c232] has no effect on ethanol induced hyperactivity in DopR[f02676] homozygous mutants. (Kong et al., 2010) DopR[f02676] mutants show reduced ethanol-induced hyperactivity and no change in ethanol sedation sensitivity or ethanol absorption. DopR[f02676] also show increased activity prior to ethanol exposure. DopR[f02676]/Df(3R)ED5634 flies show reduced ethanol-induced hyperactivity. DopR[f02676] mutants perform indistinguishably from wild-type in a rapid tolerance paradigm, showing both increased ethanol-induced hyperactivity and increased resistance to the sedating effects of ethanol during a second exposure to ethanol vapor. (Kong et al., 2010) The failure of DopR[f02676]/In(3LR)234 or DopR[f02676]/DopR[f02676] adults to learn to avoid specific odors after conditioning by concurrent shock and odor exposure is partially rescued by Scer\GAL4[elav-C155] and completely rescued by Scer\GAL4[Mef2.247]. Scer\GAL4[elav-C155] and Scer\GAL4[Mef2.247] also completely rescued learning by sugar-reward conditioning in these flies. (In all cases, rescue was tested in the presence of Scer\GAL80[ts.αTub84B] and rescued was seen at 30\'C but not at room temperature.) (Kim et al., 2007) DopR[f02676]/DopR[f02676] and DopR[f02676]/In(3LR)234 adults fail to learn to avoid specific odors after conditioning by concurrent shock and odor exposure. The same results are seen when testing is done immediately after conditioning or 1 hour after conditioning and with a variety of odors. These animals also have significantly reduced scores in tests for sugar reward mediated olfactory conditioning. (Kim et al., 2007)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	f02676 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Harvard	- PBac{WH}DopR^f02676
Linkouts
Comments
	Location 3R:10032116-10032117 confirmed by FlyBase alignment of dbGSS accession CZ485220 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	f02676 (Kim et al., 2007, Kong et al., 2010) PBac{WH}DopR^f02676 (FlyBase, 2005, FlyBase, 1992-, ) PBac{WH}f02676 (Thibault, 2004, FlyBase, 1992-) UAS-DopR1 (Inagaki et al., 2012)
Secondary FlyBase IDs

References ( 14 )
Research paper	Inagaki et al., 2012, Cell 148(3): 583--595 Visualizing Neuromodulation In Vivo: TANGO-Mapping of Dopamine Signaling Reveals Appetite Control of Sugar Sensing. [FBrf0217362] Liu et al., 2012, Curr. Biol. 22(22): 2114--2123 Two dopaminergic neurons signal to the dorsal fan-shaped body to promote wakefulness in Drosophila. [FBrf0220041] Bellen et al., 2011, Genetics 188(3): 731--743 The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229] Kong et al., 2010, PLoS ONE 5(4): e9954 A pair of dopamine neurons target the D1-like dopamine receptor DopR in the central complex to promote ethanol-stimulated locomotion in Drosophila. [FBrf0210514] Kim et al., 2007, J. Neurosci. 27(29): 7640--7647 D1 dopamine receptor dDA1 is required in the mushroom body neurons for aversive and appetitive learning in Drosophila. [FBrf0202085] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Thibault et al., 2004, Nat. Genet. 36(3): 283--287 A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material	Thibault, 2004, Nature Genetics 36(3): Supplementary Table 3. [FBrf0174228]
Personal communication to FlyBase	Christensen et al., 2008.4.15, Isolation and characterization of Df(3R)BSC470. Isolation and characterization of Df(3R)BSC470. [FBrf0204548] Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. (Computer file) Genomic mapping of Exelixis insertion collection. (Computer file) [FBrf0184340] Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177]
FlyBase analysis	FlyBase Curators, 2013, Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. [FBrf0221061] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\PBac{WH}DopRf02676 Insertion

Dmel\PBac{WH}DopR^f02676 Insertion