A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\PBac{WH}v(2)k05816f02757 Insertion

General Information
Symbol Dmel\PBac{WH}v(2)k05816f02757 Species D. melanogaster
Name FlyBase ID FBti0050808
Feature type transposable_element_insertion_site
Description
Inserted element PBac{WH} Expression data
Affected gene(s) v(2)k05816 Viability / fertility
Causes allele(s) v(2)k05816f02757 Stock availability 1 publicly available
LINE ID f02757
Genomic Location
Chromosomal location 2L ( 23C5 ) Sequence location 2L:3,054,820..3,054,820 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBti0131757 FBti0112078 FBti0106648 FBti0055433 FBti0105873 FBti0033482 FBti0006767 FBti0112909 FBti0106539 FBti0027105 FBti0007668 FBti0050808 FBti0067513 FBti0046123 FBti0046230 FBti0108795 FBti0029668 FBti0024467 FBti0029843 FBti0102959 FBti0106211 FBti0102279 FBti0007638 FBti0029794 FBti0102284 FBti0110713 FBti0102636 FBti0066296 FBti0066067 FBti0045815 FBti0107019 FBti0066295 FBti0103030 FBti0110917 FBti0112930 FBti0100819 FBti0033325 FBti0109306 FBti0110325 FBti0071040 FBti0054779 FBti0110866 FBti0052486 FBti0034000 FBti0056291 FBti0109524 FBti0059082 FBti0021283 FBti0105289 FBti0109397 FBti0036415 FBti0025916 FBti0055656 FBti0109558 FBti0109536
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the Tni\piggyBac-based construct PBac{WH}. The PBac{WH} construct carries the w[+mC] mini-white marker, a single long (199-bp) Scer\FRT site, and a terminal Scer\UAS site for Scer\GAL4-driven misexpression of adjacent genes. The FRT site allows Scer\FLP-mediated recombination between other FRT-containing elements, and thus can be used to generate molecularly defined deletions.
PBac{WH} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Thibault et al., 2004, Bellen et al., 2011)
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
2L
(Thibault, 2004)
Sequence Location
2L:3,054,820..3,054,820 [-]
(Gene Disruption Project members and Exelixis, 2005)
Orientation
-
(Thibault, 2004)
Cytological location
(computed by FlyBase)
23C5 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
23D1 (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
Comments concerning
location
hide Sequence Data
Flanking sequence
CZ485306
(Gene Disruption Project members, 2001-)
hide Inserted Element
Construct PBac{WH}
Location-dependent
role
deletion generation component
(Thibault et al., 2004)
mobile activating element
(Thibault et al., 2004)
carries FRT site(s)
(Thibault et al., 2004)
Size 7.234Kb
Associated alleles
gypsy\su(Hw)BR348
Scer\UAS14x.hs.XP.Scer\FRT
Scer\UAS14x.hs.XP
TrlBRcRa
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
v(2)k05816
(Gene Disruption Project members, 2001-)
hide Alleles and Phenotypes
Causes alleles
v(2)k05816f02757
(Gene Disruption Project members and Exelixis, 2005)
Lethality
References
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
f02757
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Harvard
-
PBac{WH}v(2)k05816f02757
hide Linkouts
hide Comments
Location 2L:3054820-3054821 confirmed by FlyBase alignment of dbGSS accession CZ485306 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
PBac{WH}f02757
(Thibault, 2004, FlyBase, 1992-)
PBac{WH}v(2)k05816f02757
(FlyBase, 2005, FlyBase, 1992-, )
Secondary FlyBase IDs
hide References ( 9 )
Research paper
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Thibault et al., 2004, Nat. Genet. 36(3): 283--287
A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material
Thibault, 2004, Nature Genetics 36(3):
Supplementary Table 3. [FBrf0174228]
Personal communication to FlyBase
Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. (Computer file)
Genomic mapping of Exelixis insertion collection. (Computer file) [FBrf0184340]
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
FlyBase analysis
FlyBase Curators, 2013, Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}.
Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. [FBrf0221061]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]