A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\PBac{WH}Pex6f03888 Insertion

General Information
Symbol Dmel\PBac{WH}Pex6f03888 Species D. melanogaster
Name FlyBase ID FBti0051497
Feature type transposable_element_insertion_site
Description
Inserted element PBac{WH} Expression data
Affected gene(s) Pex6 Viability / fertility
Causes allele(s) Pex6f03888 Stock availability 1 publicly available
LINE ID f03888
Genomic Location
Chromosomal location 2R ( 47C5 ) Sequence location 2R:6,771,583..6,771,596 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0062309 FBti0056079 FBti0007623 FBti0111347 FBti0028386 FBti0107945 FBti0021105 FBti0072769 FBti0105169 FBti0011132 FBti0110476 FBti0039965 FBti0105600 FBti0058901 FBti0025217 FBti0026261 FBti0011170 FBti0006379 FBti0034316 FBti0146096 FBti0103258 FBti0109518 FBti0028193 FBti0027163 FBti0056898 FBti0007604 FBti0010979 FBti0011067 FBti0106312 FBti0103576 FBti0107026 FBti0106943 FBti0030290 FBti0030313 FBti0106405 FBti0077118 FBti0113283 FBti0111948 FBti0103089 FBti0112986 FBti0005347 FBti0046097 FBti0044177 FBti0042127 FBti0109870 FBti0110710 FBti0040135 FBti0025844 FBti0070822 FBti0018849 FBti0025218 FBti0114804 FBti0051497 FBti0038944 FBti0053460 FBti0125099_1 FBti0125099_2 FBti0109994 FBti0025768 FBti0042089 FBti0108651
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the Tni\piggyBac-based construct PBac{WH}. The PBac{WH} construct carries the w[+mC] mini-white marker, a single long (199-bp) Scer\FRT site, and a terminal Scer\UAS site for Scer\GAL4-driven misexpression of adjacent genes. The FRT site allows Scer\FLP-mediated recombination between other FRT-containing elements, and thus can be used to generate molecularly defined deletions.
PBac{WH} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Thibault et al., 2004, Bellen et al., 2011)
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Description
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FB2013_03
FB2013_02
All updates Click here to see a list of all updates to this record from FB2010_08 and on.
hide Detailed Mapping Data
Chromosome (arm)
2R
(Thibault, 2004)
Sequence Location
2R:6,771,583..6,771,596 [+]
(Gene Disruption Project members and Exelixis, 2005)
Orientation
+
(Thibault, 2004)
Cytological location
(computed by FlyBase)
47C5 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
47C3 (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
Comments concerning
location
hide Sequence Data
Flanking sequence
CZ486491
(Gene Disruption Project members, 2001-)
CZ486492
(Gene Disruption Project members, 2001-)
hide Inserted Element
Construct PBac{WH}
Location-dependent
role
deletion generation component
(Thibault et al., 2004)
mobile activating element
(Thibault et al., 2004)
carries FRT site(s)
(Thibault et al., 2004)
Size 7.234Kb
Associated alleles
gypsy\su(Hw)BR348
Scer\UAS14x.hs.XP.Scer\FRT
Scer\UAS14x.hs.XP
TrlBRcRa
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Pex6
(FlyBase Curators, 2008)
hide Alleles and Phenotypes
Causes alleles
Pex6f03888
(FlyBase Curators, 2008)
Lethality
References
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
f03888
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Harvard
-
PBac{WH}Pex6f03888
hide Linkouts
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
PBac{WH}CG11919f03888
 
PBac{WH}f03888
(Thibault, 2004, FlyBase, 1992-)
PBac{WH}Pex6f03888
 
Secondary FlyBase IDs
hide References ( 9 )
Research paper
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Thibault et al., 2004, Nat. Genet. 36(3): 283--287
A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material
Thibault, 2004, Nature Genetics 36(3):
Supplementary Table 3. [FBrf0174228]
Personal communication to FlyBase
Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. (Computer file)
Genomic mapping of Exelixis insertion collection. (Computer file) [FBrf0184340]
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
FlyBase analysis
FlyBase Curators, 2013, Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}.
Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. [FBrf0221061]
FlyBase Curators, 2008, Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations.
Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. [FBrf0202955]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]