FB2025_01 , released February 20, 2025
Insertion: Dmel\PBac{WH}Top2f05145
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General Information
Symbol
Dmel\PBac{WH}Top2f05145
Species
D. melanogaster
Name
FlyBase ID
FBti0052217
Feature type
transposable_element_insertion_site
Description
Inserted Element
PBac{WH}
LINE ID

f05145

Affected gene(s)
CG31699, Top2
Viability / fertility
Associated allele(s)
Top2f05145
Stock availability
None publicly available
Genomic Location
Chromosomal Location
2L ( 37E1 )
Sequence Location
2L:19,452,991..19,452,991 [+]
Target / Docking site
Genomic Maps
Member of Large Scale Dataset(s)
Dataset
TI_set_PBac{WH}.Exel.f
Description

PBac{WH} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.

(Bellen et al., 2011)

A set of transgenic insertion stocks derived by TE mobilization using the Tni\piggyBac-based construct PBac{WH}. The PBac{WH} construct carries the w+mC mini-white marker, a single long (199-bp) Scer\FRT site, and a terminal Scer\UAS site for Scer\GAL4-driven misexpression of adjacent genes. The FRT site allows Scer\FLP-mediated recombination between other FRT-containing elements, and thus can be used to generate molecularly defined deletions.

(Thibault et al., 2004)
Detailed Mapping Data
Chromosome (arm)

2L

(Thibault, 2004)
Sequence Location
2L:19,452,991..19,452,991 [+]
(Gene Disruption Project members and Exelixis, 2005)
Orientation

+

(Thibault, 2004)
Cytological location (computed by FlyBase)
37E1 (inferred by FlyBase from sequence location)
Cytological location (reported)
37E1 (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Inserted Element
Construct
PBac{WH}
Size (Kb)

7.234

Component Allele(s)
w+mC
Molecular map
Affected Gene(s)
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Data on Genetic Line
Line ID

f05145

(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (0)
External Crossreferences and Linkouts ( 0 )
Comments
Comments

Location 2L:19448920-19448921 confirmed by FlyBase alignment of dbGSS accession CZ487766 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.

(FlyBase, 2005)
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
PBac{WH}Top2f05145
(FlyBase, 2005, FlyBase, 1992-)
PBac{WH}f05145
(Thibault, 2004, FlyBase, 1992-)
Top2f05145
(Ramos et al., 2011)
Name Synonyms
Secondary FlyBase IDs
    References (9)