A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\PBac{WH}CG9003f07493 Insertion

General Information
Symbol Dmel\PBac{WH}CG9003f07493 Species D. melanogaster
Name FlyBase ID FBti0053524
Feature type transposable_element_insertion_site
Description
Inserted element PBac{WH} Expression data
Affected gene(s) CG9003 Viability / fertility
Causes allele(s) CG9003f07493 Stock availability 1 publicly available
LINE ID f07493
Genomic Location
Chromosomal location 2R ( 48B2 ) Sequence location 2R:7,536,275..7,536,275 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBti0065325 FBti0044328 FBti0066878 FBti0047751 FBti0067100 FBti0067273 FBti0047533 FBti0043920 FBti0043300 FBti0067669 FBti0059073 FBti0003180 FBti0105778 FBti0110056 FBti0106592 FBti0054785 FBti0042941 FBti0151391 FBti0036951 FBti0025887 FBti0023361 FBti0106738 FBti0069297 FBti0029535 FBti0103469 FBti0039949 FBti0054944 FBti0051709 FBti0053524 FBti0105205 FBti0110074 FBti0105692 FBti0105076 FBti0076143 FBti0103955 FBti0125110 FBti0049878 FBti0051875 FBti0125111_2 FBti0125111_1 FBti0107102 FBti0011175 FBti0029631 FBti0021247
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the Tni\piggyBac-based construct PBac{WH}. The PBac{WH} construct carries the w[+mC] mini-white marker, a single long (199-bp) Scer\FRT site, and a terminal Scer\UAS site for Scer\GAL4-driven misexpression of adjacent genes. The FRT site allows Scer\FLP-mediated recombination between other FRT-containing elements, and thus can be used to generate molecularly defined deletions.
PBac{WH} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Thibault et al., 2004, Bellen et al., 2011)
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
2R
(Thibault, 2004)
Sequence Location
2R:7,536,275..7,536,275 [-]
(Gene Disruption Project members and Exelixis, 2005)
Orientation
-
(Thibault, 2004)
Cytological location
(computed by FlyBase)
48B2 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
48B2 (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
Comments concerning
location
hide Sequence Data
Flanking sequence
CZ490225
(Gene Disruption Project members, 2001-)
hide Inserted Element
Construct PBac{WH}
Location-dependent
role
deletion generation component
(Thibault et al., 2004)
mobile activating element
(Thibault et al., 2004)
carries FRT site(s)
(Thibault et al., 2004)
Size 7.234Kb
Associated alleles
gypsy\su(Hw)BR348
Scer\UAS14x.hs.XP.Scer\FRT
Scer\UAS14x.hs.XP
TrlBRcRa
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
CG9003
(FlyBase Curators, 2008)
hide Alleles and Phenotypes
Causes alleles
CG9003f07493
(FlyBase Curators, 2008)
Lethality
References
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
f07493
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Harvard
-
PBac{WH}CG9003f07493
hide Linkouts
hide Comments
Location 2R:7164677-7164678 confirmed by FlyBase alignment of dbGSS accession CZ490225 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
PBac{WH}CG9003f07493
 
PBac{WH}f07493
(Thibault, 2004, FlyBase, 2005, FlyBase, 1992-)
Secondary FlyBase IDs
hide References ( 10 )
Research paper
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Thibault et al., 2004, Nat. Genet. 36(3): 283--287
A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material
Thibault, 2004, Nature Genetics 36(3):
Supplementary Table 3. [FBrf0174228]
Personal communication to FlyBase
Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. (Computer file)
Genomic mapping of Exelixis insertion collection. (Computer file) [FBrf0184340]
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
FlyBase analysis
FlyBase Curators, 2013, Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}.
Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. [FBrf0221061]
FlyBase Curators, 2008, Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations.
Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. [FBrf0202955]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]