A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{XP}sdkd02333 Insertion

General Information
Symbol Dmel\P{XP}sdkd02333 Species D. melanogaster
Name FlyBase ID FBti0054829
Feature type transposable_element_insertion_site
Description
Inserted element P{XP} Expression data
Affected gene(s) sdk Viability / fertility
Causes allele(s) sdkd02333 Stock availability 1 publicly available
LINE ID d02333
Genomic Location
Chromosomal location X ( 1C2 ) Sequence location X:582,079..582,079 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0063326 FBti0063328 FBti0063332 FBti0071276 FBti0043614 FBti0048804 FBti0055628 FBti0110767 FBti0109673 FBti0069937 FBti0029281 FBti0036790 FBti0040047 FBti0034133 FBti0007299_2 FBti0007299_1 FBti0021734 FBti0055370 FBti0035788 FBti0035480 FBti0035174 FBti0071999 FBti0049180 FBti0108633 FBti0015113 FBti0035696 FBti0029582 FBti0104371 FBti0034113 FBti0054829 FBti0034453 FBti0143954 FBti0075932 FBti0109067
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{XP}. The P{XP} construct carries the w[+mC] mini-white marker, long (199-bp) Scer\FRT sites, and is designed to allow flexible use of Scer\UAS sites for Scer\GAL4-driven misexpression of adjacent genes. FRT sites allow Scer\FLP-mediated recombination between other FRT-containing elements, and thus can be used to generate molecularly defined deletions.
P{XP} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Thibault et al., 2004, Bellen et al., 2011)
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
X
(Thibault, 2004)
Sequence Location
X:582,079..582,079 [+]
(Gene Disruption Project members and Exelixis, 2005)
Orientation
+
(Thibault, 2004)
Cytological location
(computed by FlyBase)
1C2 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
1C2 (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
Comments concerning
location
hide Sequence Data
Flanking sequence
CZ472758
(Gene Disruption Project members, 2001-)
hide Inserted Element
Construct P{XP}
Location-dependent
role
deletion generation component
(Thibault et al., 2004)
mobile activating element
(Thibault et al., 2004)
carries FRT site(s)
(Thibault et al., 2004)
Size 7.303Kb
Associated alleles
gypsy\su(Hw)BR348
Scer\UAS14x.hs.XP.Scer\FRT
Scer\UAS14x.hs.XP
TrlBRcRa
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
sdk
(Gene Disruption Project members, 2001-)
hide Alleles and Phenotypes
Causes alleles
sdkd02333
(Gene Disruption Project members and Exelixis, 2005)
Lethality
References
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
d02333
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Harvard
-
P{XP}sdkd02333
hide Linkouts
hide Comments
Location X:544867-544868 confirmed by FlyBase alignment of dbGSS accession CZ472758 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{XP}d02333
(Thibault, 2004, FlyBase, 1992-)
P{XP}sdkd02333
(FlyBase, 2005, FlyBase, 1992-, )
Secondary FlyBase IDs
hide References ( 9 )
Research paper
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Thibault et al., 2004, Nat. Genet. 36(3): 283--287
A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material
Thibault, 2004, Nature Genetics 36(3):
Supplementary Table 3. [FBrf0174228]
Personal communication to FlyBase
Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. (Computer file)
Genomic mapping of Exelixis insertion collection. (Computer file) [FBrf0184340]
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
FlyBase analysis
FlyBase Curators, 2013, Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}.
Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. [FBrf0221061]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]