A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{XP}Rab11d04643 Insertion

General Information
Symbol Dmel\P{XP}Rab11d04643 Species D. melanogaster
Name FlyBase ID FBti0055598
Feature type transposable_element_insertion_site
Description
Inserted element P{XP} Expression data
Affected gene(s) Rab11 Viability / fertility
Causes allele(s) Rab11d04643 Stock availability 1 publicly available
LINE ID d04643
Genomic Location
Chromosomal location 3R ( 93B13 ) Sequence location 3R:16,938,733..16,938,733 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBti0042974 FBti0112250 FBti0039498 FBti0110097 FBti0011812 FBti0005579 FBti0052320 FBti0105295 FBti0043590 FBti0072702 FBti0059382 FBti0126195_2 FBti0126195_1 FBti0011330 FBti0004929 FBti0099842 FBti0057504 FBti0037087 FBti0033983 FBti0054699 FBti0143543 FBti0048125 FBti0055598 FBti0065517 FBti0044705 FBti0004986 FBti0005448_2 FBti0111338 FBti0005448_1
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{XP}. The P{XP} construct carries the w[+mC] mini-white marker, long (199-bp) Scer\FRT sites, and is designed to allow flexible use of Scer\UAS sites for Scer\GAL4-driven misexpression of adjacent genes. FRT sites allow Scer\FLP-mediated recombination between other FRT-containing elements, and thus can be used to generate molecularly defined deletions.
P{XP} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Thibault et al., 2004, Bellen et al., 2011)
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
3R
(Thibault, 2004)
Sequence Location
3R:16,938,733..16,938,733 [-]
(Gene Disruption Project members and Exelixis, 2005)
Orientation
-
(Thibault, 2004)
Cytological location
(computed by FlyBase)
93B13 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
93B13 (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
Comments concerning
location
hide Sequence Data
Flanking sequence
CZ474047
(Gene Disruption Project members, 2001-)
hide Inserted Element
Construct P{XP}
Location-dependent
role
deletion generation component
(Thibault et al., 2004)
mobile activating element
(Thibault et al., 2004)
carries FRT site(s)
(Thibault et al., 2004)
Size 7.303Kb
Associated alleles
gypsy\su(Hw)BR348
Scer\UAS14x.hs.XP.Scer\FRT
Scer\UAS14x.hs.XP
TrlBRcRa
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Rab11
(Gene Disruption Project members, 2001-, Cheli et al., 2010)
hide Alleles and Phenotypes
Causes alleles
Rab11d04643
(Gene Disruption Project members and Exelixis, 2005, Cheli et al., 2010)
Lethality
References
lethal | recessive
(Lighthouse et al., 2008)
Sterility
References
female sterile | germline clone
(Lighthouse et al., 2008)
hide Phenotype Manifest In
germline stem cell | germline clone
(Lighthouse et al., 2008)
hide Detailed Description
Statement
Reference
Females carrying homozygous germline clones are sterile. Germline stem cell clones show an approximately 4-fold reduction in half-life compared to control clones. Homozygous clones in the adult germ cells result in defective fusomes. The number of tubulovesicular structures in the fusomes is markedly reduced in the mutant germline stem cells compared to controls.
(Lighthouse et al., 2008)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
d04643
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Harvard
-
P{XP}Rab11d04643
hide Linkouts
hide Comments
Location 3R:16938733-16938734 confirmed by FlyBase alignment of dbGSS accession CZ474047 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{XP}d04643
(Thibault, 2004, FlyBase, 1992-)
P{XP}Rab11d04643
(FlyBase, 2005, FlyBase, 1992-, Cheli et al., 2010, )
Secondary FlyBase IDs
hide References ( 11 )
Research paper
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Cheli et al., 2010, Hum. Mol. Genet. 19(5): 861--878
Genetic modifiers of abnormal organelle biogenesis in a Drosophila model of BLOC-1 deficiency. [FBrf0209903]
Lighthouse et al., 2008, Dev. Biol. 317(1): 59--71
New components of the Drosophila fusome suggest it plays novel roles in signaling and transport. [FBrf0211303]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Thibault et al., 2004, Nat. Genet. 36(3): 283--287
A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material
Thibault, 2004, Nature Genetics 36(3):
Supplementary Table 3. [FBrf0174228]
Personal communication to FlyBase
Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. (Computer file)
Genomic mapping of Exelixis insertion collection. (Computer file) [FBrf0184340]
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
FlyBase analysis
FlyBase Curators, 2013, Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}.
Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. [FBrf0221061]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]