Dmel\P{XP}Sin3A^d07432 Insertion

General Information
Symbol	Dmel\P{XP}Sin3A^d07432	Species	D. melanogaster
Name		FlyBase ID	FBti0056406
Feature type	transposable_element_insertion_site
Description
Inserted element	P{XP}	Expression data
Affected gene(s)	Sin3A	Viability / fertility
Causes allele(s)	Sin3A^d07432	Stock availability	1 publicly available
LINE ID	d07432
Genomic Location
Chromosomal location	2R ( 49B7 )	Sequence location	2R:8,474,190..8,474,190 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{XP}.Exel.d A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{XP}. The P{XP} construct carries the w[+mC] mini-white marker, long (199-bp) Scer\FRT sites, and is designed to allow flexible use of Scer\UAS sites for Scer\GAL4-driven misexpression of adjacent genes. FRT sites allow Scer\FLP-mediated recombination between other FRT-containing elements, and thus can be used to generate molecularly defined deletions. P{XP} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank. (Thibault et al., 2004, Bellen et al., 2011)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)	2R (Thibault, 2004)
Sequence Location	2R:8,474,190..8,474,190 [+] (Gene Disruption Project members and Exelixis, 2005)
Orientation	+ (Thibault, 2004)
Cytological location (computed by FlyBase)	49B7 ( inferred by FlyBase from sequence location )
Cytological location (reported)	49B7 (reported as inferred from sequence location) (Gene Disruption Project members, 2001-)
Comments concerning location
Sequence Data
Flanking sequence	CZ475731 (Gene Disruption Project members, 2001-)
Inserted Element
Construct	P{XP}
Location-dependent role	deletion generation component (Thibault et al., 2004) mobile activating element (Thibault et al., 2004) carries FRT site(s) (Thibault et al., 2004)
Size	7.303Kb
Associated alleles	gypsy\su(Hw)BR³⁴⁸ Scer\UAS^{14x.hs.XP.Scer\FRT} Scer\UAS^14x.hs.XP TrlBR^cRa w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Sin3A (Gene Disruption Project members, 2001-, Kankel et al., 2007)
Alleles and Phenotypes
Causes alleles	Sin3A^d07432 (Gene Disruption Project members and Exelixis, 2005, Kankel et al., 2007)

Lethality References
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference Sin3A[d07432] dominantly enhances the wing nicking phenotype caused by expression of mam[N.Scer\UAS] under the control of Scer\GAL4[bbg-C96]. (Kankel et al., 2007)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	d07432 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Harvard	- P{XP}Sin3A^d07432
Linkouts
Comments
	Location 2R:8102591-8102592 refined by FlyBase alignment of dbGSS accession CZ475731 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	d07432 (Kankel et al., 2007) P{XP}d07432 (Thibault, 2004, FlyBase, 1992-) P{XP}Sin3A^d07432 (FlyBase, 2005, FlyBase, 1992-, )
Secondary FlyBase IDs

References ( 10 )
Research paper	Bellen et al., 2011, Genetics 188(3): 731--743 The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229] Kankel et al., 2007, Genetics 177(4): 2493--2505 Investigating the genetic circuitry of mastermind in Drosophila, a notch signal effector. [FBrf0202064] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Thibault et al., 2004, Nat. Genet. 36(3): 283--287 A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material	Thibault, 2004, Nature Genetics 36(3): Supplementary Table 3. [FBrf0174228]
Personal communication to FlyBase	Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. (Computer file) Genomic mapping of Exelixis insertion collection. (Computer file) [FBrf0184340] Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177]
FlyBase analysis	FlyBase Curators, 2013, Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. [FBrf0221061] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{XP}Sin3Ad07432 Insertion

Dmel\P{XP}Sin3A^d07432 Insertion