A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{XP}GstD1d04829 Insertion

General Information
Symbol Dmel\P{XP}GstD1d04829 Species D. melanogaster
Name FlyBase ID FBti0057450
Feature type transposable_element_insertion_site
Description
Inserted element P{XP} Expression data
Affected gene(s) GstD1 Viability / fertility
Causes allele(s) Stock availability none publicly available
LINE ID d04829
Genomic Location
Chromosomal location 3R ( 87B8 ) Sequence location 3R:8,194,980..8,194,980 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0061002 FBti0061004 FBti0041216 FBti0042616 FBti0046272 FBti0052073 FBti0114825 FBti0070581 FBti0110037 FBti0056389 FBti0057450 FBti0053985 FBti0037511 FBti0069577 FBti0024927 FBti0067399 FBti0049375 FBti0047303 FBti0065491 FBti0065358 FBti0037062 FBti0018599 FBti0007853 FBti0106005 FBti0057010 FBti0100836 FBti0055283 FBti0051057 FBti0041912 FBti0042862 FBti0056869 FBti0040198 FBti0068572 FBti0100768 FBti0070128 FBti0023593 FBti0102616 FBti0029024 FBti0069924 FBti0057363 FBti0128304 FBti0046901 FBti0075598
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{XP}. The P{XP} construct carries the w[+mC] mini-white marker, long (199-bp) Scer\FRT sites, and is designed to allow flexible use of Scer\UAS sites for Scer\GAL4-driven misexpression of adjacent genes. FRT sites allow Scer\FLP-mediated recombination between other FRT-containing elements, and thus can be used to generate molecularly defined deletions.
P{XP} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Thibault et al., 2004, Bellen et al., 2011)
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
3R:8,194,980..8,194,980 [+]
(Gene Disruption Project members and Exelixis, 2005)
Orientation
Cytological location
(computed by FlyBase)
87B8 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
87B8 (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
87B8 (reported as inferred from sequence location)
(Parks et al., 2004)
Comments concerning
location
hide Sequence Data
Flanking sequence
CZ474159
(Gene Disruption Project members, 2001-)
hide Inserted Element
Construct P{XP}
Location-dependent
role
deletion generation component
(Thibault et al., 2004)
mobile activating element
(Thibault et al., 2004)
carries FRT site(s)
(Thibault et al., 2004)
Size 7.303Kb
Associated alleles
gypsy\su(Hw)BR348
Scer\UAS14x.hs.XP.Scer\FRT
Scer\UAS14x.hs.XP
TrlBRcRa
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
GstD1
(Gene Disruption Project members, 2001-)
hide Alleles and Phenotypes
Causes alleles
Lethality
References
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
d04829
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 0 )
hide Linkouts
hide Comments
Location 3R:8194980-8194981 confirmed by FlyBase alignment of dbGSS accession CZ474159 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{XP}d04829
(Parks et al., 2004, FlyBase, 1992-)
P{XP}GstD1d04829
(FlyBase, 2005, FlyBase, 1992-, )
Secondary FlyBase IDs
hide References ( 10 )
Research paper
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Parks et al., 2004, Nat. Genet. 36(3): 288--292
Systematic generation of high-resolution deletion coverage of the Drosophila melanogaster genome. [FBrf0175003]
Thibault et al., 2004, Nat. Genet. 36(3): 283--287
A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material
Parks et al., 2004, Nature Genetics 36(3):
Supplementary Table 2. [FBrf0174230]
Personal communication to FlyBase
Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. (Computer file)
Genomic mapping of Exelixis insertion collection. (Computer file) [FBrf0184340]
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
FlyBase analysis
FlyBase Curators, 2013, Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}.
Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. [FBrf0221061]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]