Dmel\P{EPgy2}pAbp^EY11561 Insertion

General Information
Symbol	Dmel\P{EPgy2}pAbp^EY11561	Species	D. melanogaster
Name		FlyBase ID	FBti0057651
Feature type	transposable_element_insertion_site
Description
Inserted element	P{EPgy2}	Expression data
Affected gene(s)	pAbp	Viability / fertility
Causes allele(s)	pAbp^EY11561	Stock availability	1 publicly available
LINE ID	EY11561
Genomic Location
Chromosomal location	2R ( 55B8 )	Sequence location	2R:14,028,250..14,028,250 [+]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	2R:14,028,250..14,028,250 [+] (Gene Disruption Project members, 2001-)
Orientation
Cytological location (computed by FlyBase)	55B8 ( inferred by FlyBase from sequence location )
Cytological location (reported)	55B8 (reported as inferred from sequence location) (Gene Disruption Project members, 2001-)
Comments concerning location
Sequence Data
Flanking sequence	CL868500 (Gene Disruption Project members, 2001-)
Inserted Element
Construct	P{EPgy2}
Location-dependent role	mobile activating element (UASG) (Bellen et al., 2004)
Size	10.908Kb
Associated alleles	Scer\UAS^14x.P\T TrlBR^cRa w^+mC y^+mDint2
Molecular map
Affected Gene(s)
Insertion may affect gene	pAbp (Gene Disruption Project members, 2001-, Cziko et al., 2009, Pertceva et al., 2010)
Alleles and Phenotypes
Causes alleles	pAbp^EY11561 (Gene Disruption Project members, 2001-, Cziko et al., 2009, Pertceva et al., 2010)

Lethality References viable (Blagden et al., 2009)
Sterility References female fertile (Blagden et al., 2009) male sterile (Blagden et al., 2009)
Phenotype Manifest In
	centriole & spermatocyte (Blagden et al., 2009) class IV dendritic arborizing neuron \| somatic clone (Cziko et al., 2009) elongation stage spermatid (Pertceva et al., 2010) onion stage spermatid (Pertceva et al., 2010) spindle & spermatocyte (Blagden et al., 2009)
Detailed Description
Statement Reference pAbp[k10109]/pAbp[EY11561] heterozygotes exhibit abnormalities in spermatogenesis. these include absence of meiotic cytokinesis (in 5% of cases), resulting in formation of polyploid spermatids and defects in spermatid elongation (in approximately 100% of cases). Nuclei fail to elongate, keep a round shape and are dispersed along the cyst instead of grouping at the head of the cyst. Nebenkern DAPi staining (for DNA) appears to be non-uniform in these mutants, reflecting the effect of the trans-heterozygous mutants on mitochondrial DNA organization in at least 60% of cases. There appears to be no difference between the trans-heterozygous mutant and controls, showing that mutant mitochondrial membranes keep their integrity, but in the mutant, the onion-stage Nebenkern looks larger than in controls, and its morphology differs from a perfect sphere. Examination with an electron microscope reveals abnormal mitochondrial membranes at onion-stage spermatids in the trans-heterozygous mutant. These mitochondria also exhibit defective attachment to axonemes in the elongating spermatids. (Pertceva et al., 2010) pAbp[EY11561]/Df(2R)Pcl7B spermatocytes show defects in meiosis; some spermatocytes have short spindles, and premature separation of centrioles is seen in some cases. (Blagden et al., 2009) Over-expression of pAbp[EY11561] reduces dendrite branching, and alters dendritic morphology in larval class IV sensory neurons (induced under the control of Scer\GAL4[Act.PU]). Total dendrite length is not significantly reduced in these neurons. (Cziko et al., 2009)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	EY11561 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	20684 y¹ w^67c23; P{EPgy2}pAbp^EY11561
Linkouts
Comments
	Location 2R:13655608-13655609 confirmed by FlyBase alignment of dbGSS accession CL868500 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{EPgy2}EY11561 (FlyBase, 1992-) P{EPgy2}pAbp^EY11561 (FlyBase, 2005, FlyBase, 1992-, Pertceva et al., 2010) PABP EY11561 (Cziko et al., 2009)
Secondary FlyBase IDs

References ( 7 )
Research paper	Pertceva et al., 2010, Cell Biol. Int. 34(10): 991--996 The role of Drosophila hyperplastic discs gene in spermatogenesis. [FBrf0211767] Blagden et al., 2009, Dev. Biol. 334(1): 186--197 Drosophila Larp associates with poly(A)-binding protein and is required for male fertility and syncytial embryo development. [FBrf0208825] Cziko et al., 2009, Genetics 182(4): 1051--1060 Genetic Modifiers of dFMR1 Encode RNA Granule Components in Drosophila. [FBrf0208636] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177]

Dmel\P{EPgy2}pAbpEY11561 Insertion

Dmel\P{EPgy2}pAbp^EY11561 Insertion