Additional P{EPgy2} insertion lines were generated, mapped, and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EPgy2}; created and vetted by the Gene Disruption Project (GDP). The P{EPgy2} construct carries two visible markers, the mini-white marker w+mC and the mini-yellow marker y+mDint2, and Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.
Third instar larvae expressing P{EPgy2}EY14738a under the control of Scer\GAL4Pxn.PS have a normal number of sessile hemocytes. The number of hemocytes in circulation is also similar to wild type. The dorsal sessile hemocyte department is disrupted. Inappropriate targeting of hemocytes is seen, but sessile hemocytes do not accumulate along the dorsal vessel or spread through the cuticle. The lymph gland is enlarged. Hemocyte shape is normal.
Location 2L:7590421-7590422 confirmed by FlyBase alignment of dbGSS accession CW848273 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.