Dmel\PBac{GAL4D,EYFP}PL00074 Insertion

General Information
Symbol	Dmel\PBac{GAL4D,EYFP}PL00074	Species	D. melanogaster
Name		FlyBase ID	FBti0058095
Feature type	transposable_element_insertion_site
Description
Inserted element	PBac{GAL4D,EYFP}	Expression data
Affected gene(s)		Viability / fertility	fertile, viable
Causes allele(s)		Stock availability	1 publicly available
LINE ID	PL00074
Genomic Location
Chromosomal location	3R ( 81F )	Sequence location
Member of Large Scale Dataset(s)
Dataset	TI_set_PBac{GAL4D,EYFP}.PL A set of transgenic insertion stocks derived by TE mobilization using the "piggyBac mutator" construct PBac{GAL4D,EYFP}. The dataset consists primarily of insertions on the third chromosomes that also carry proximal Scer\FRT sites, to allow generation of germ-line clones. The PBac{GAL4D,EYFP} construct carries the yellow fluorescent marker Avic\GFP[EYFP.3xP3] (which can be distinguished from other GFP-based markers) and a modified Scer\GAL4 driver/reporter gene. Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection; some lines were mapped by the GDP. (Hacker et al., 2003, Bellen et al., 2004)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)
Cytological location (reported)	81F (reported as inferred from sequence location) (Haecker et al., 2004)
Comments concerning location
Sequence Data
Flanking sequence	CZ559496 (FlyBase, 1992-) CZ559497 (FlyBase, 1992-)
Inserted Element
Construct	PBac{GAL4D,EYFP}
Location-dependent role	GAL4 driver/enhancer trap (Horn et al., 2003)
Size
Associated alleles	Avic\GFP^EYFP.3xP3 Scer\GAL4^Δ.P\T
Molecular map
Affected Gene(s)
Insertion may affect gene
Alleles and Phenotypes
Causes alleles

Lethality References viable (Haecker et al., 2004)
Sterility References fertile (Haecker et al., 2004)
Phenotype Manifest In

Detailed Description
Statement Reference
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	PL00074 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	19374 w^*; P{FRT(w^hs)}2A PBac{GAL4D,EYFP}PL00074 P{neoFRT}82B
Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	PBac{GAL4D,EYFP}PL00074 (FlyBase, 1992-, )
Secondary FlyBase IDs

References ( 7 )
Research paper	Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Hacker et al., 2003, Proc. Natl. Acad. Sci. U.S.A. 100(13): 7720--7725 piggyBac-based insertional mutagenesis in the presence of stably integrated P elements in Drosophila. [FBrf0159706] Horn et al., 2003, Genetics 163(2): 647--661 piggyBac-based insertional mutagenesis and enhancer detection as a tool for functional insect genomics. [FBrf0158985]
Personal communication to FlyBase	Haecker et al., 2004, Haecker insertions. Haecker insertions. [FBrf0184338] Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177]
FlyBase analysis	FlyBase Curators, 2013, Members of TE insertion collections. Members of TE insertion collections. [FBrf0220668] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]