A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\PBac{GAL4D,EYFP}yuriPL00114 Insertion

General Information
Symbol Dmel\PBac{GAL4D,EYFP}yuriPL00114 Species D. melanogaster
Name FlyBase ID FBti0058224
Feature type transposable_element_insertion_site
Description
Inserted element PBac{GAL4D,EYFP} Expression data
Affected gene(s) yuri Viability / fertility fertile, viable
Causes allele(s) yuriPL00114 Stock availability 1 publicly available
LINE ID PL00114-5prime PL00114
Genomic Location
Chromosomal location 2L ( 35C5 ) Sequence location 2L:15,262,768..15,262,768 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0148746 FBti0066372 FBti0046078 FBti0066381 FBti0106746 FBti0070809 FBti0124806 FBti0046243 FBti0051348 FBti0021701 FBti0058224 FBti0028717 FBti0039764 FBti0055586 FBti0021739 FBti0109927 FBti0034077 FBti0111969 FBti0070584 FBti0011009 FBti0047514 FBti0057623 FBti0003161 FBti0141026 FBti0033462 FBti0124807
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the "piggyBac mutator" construct PBac{GAL4D,EYFP}. The dataset consists primarily of insertions on the third chromosomes that also carry proximal Scer\FRT sites, to allow generation of germ-line clones. The PBac{GAL4D,EYFP} construct carries the yellow fluorescent marker Avic\GFP[EYFP.3xP3] (which can be distinguished from other GFP-based markers) and a modified Scer\GAL4 driver/reporter gene.
Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection; some lines were mapped by the GDP.
(Hacker et al., 2003, Bellen et al., 2004)
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2L:15,262,768..15,262,768 [+]
(FlyBase, 2007.6)
Orientation
Cytological location
(computed by FlyBase)
35C5 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
35C5 (reported as inferred from sequence location)
(Haecker et al., 2004)
Comments concerning
location
hide Sequence Data
Flanking sequence
CZ559528
(FlyBase, 1992-, FlyBase, 2007.6)
hide Inserted Element
Construct PBac{GAL4D,EYFP}
Location-dependent
role
GAL4 driver/enhancer trap
(Horn et al., 2003)
Size
Associated alleles
Avic\GFPEYFP.3xP3
Scer\GAL4Δ.P\T
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
yuri
(Haecker et al., 2004)
hide Alleles and Phenotypes
Causes alleles
yuriPL00114
(Gene Disruption Project members, 2001-)
Lethality
References
viable
(Haecker et al., 2004, Gene Disruption Project members, 2001-)
Sterility
References
fertile
(Haecker et al., 2004, Gene Disruption Project members, 2001-)
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
Mutant flies show mild but significant defects in the auditory response to sound compared to wild type.
(Senthilan et al., 2012)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
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FlyView (LinkOut)
hide Data on Genetic Line
Line ID
PL00114
(Gene Disruption Project members, 2001-)
PL00114-5prime
(FlyBase, 2007.6)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
19394
w*; PBac{GAL4D,EYFP}yuriPL00114 cn1 bw1; P{FRT(whs)}2A P{neoFRT}82B
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hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
PBac{GAL4D,EYFP}CG31732[PL00114]
(FlyBase, 1992-)
PBac{GAL4D,EYFP}yuriPL00114
(FlyBase, 1992-, )
Secondary FlyBase IDs
hide References ( 9 )
Research paper
Senthilan et al., 2012, Cell 150(5): 1042--1054
Drosophila auditory organ genes and genetic hearing defects. [FBrf0219321]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Hacker et al., 2003, Proc. Natl. Acad. Sci. U.S.A. 100(13): 7720--7725
piggyBac-based insertional mutagenesis in the presence of stably integrated P elements in Drosophila. [FBrf0159706]
Horn et al., 2003, Genetics 163(2): 647--661
piggyBac-based insertional mutagenesis and enhancer detection as a tool for functional insect genomics. [FBrf0158985]
Personal communication to FlyBase
Haecker et al., 2004, Haecker insertions.
Haecker insertions. [FBrf0184338]
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
FlyBase analysis
FlyBase Curators, 2013, Members of TE insertion collections.
Members of TE insertion collections. [FBrf0220668]
FlyBase, 2007.6, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0199566]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]