Dmel\P{GawB}NP0225 Insertion

General Information
Symbol	Dmel\P{GawB}NP0225	Species	D. melanogaster
Name		FlyBase ID	FBti0058535
Feature type	transposable_element_insertion_site
Description
Inserted element	P{GawB}	Expression data	GAL4 reporter/driver
Affected gene(s)	Scer\GAL4	Viability / fertility
Causes allele(s)	Scer\GAL4^NP0225	Stock availability	1 publicly available
LINE ID
Genomic Location
Chromosomal location	2R ( 51A4 )	Sequence location	2R:10,366,536..10,366,536 [-]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset
Recent Updates
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	2R:10,366,536..10,366,536 [-] (Potter and Luo, 2010)
Orientation	- (Potter and Luo, 2010)
Cytological location (computed by FlyBase)	51A4 ( inferred by FlyBase from sequence location )
Cytological location (reported)
Comments concerning location	Approximate location; corresponds to estimated site of insertion of mdg3 element in the P{GawB}NP0225 parental strain. (Potter and Luo, 2010) Repetitive region: within mdg3 element (not found at this location in the sequenced stain). (Potter and Luo, 2010)
Sequence Data
Flanking sequence
Inserted Element
Construct	P{GawB}
Location-dependent role	GAL4 driver/enhancer trap (Brand and Perrimon, 1993)
Size	11.279Kb
Associated alleles	Scer\GAL4^wB w^+mW.hs
Molecular map
Affected Gene(s)
Insertion may affect gene	Scer\GAL4 (Tanaka et al., 2004, Masuda-Nakagawa et al., 2005, Wang et al., 2006, Grosjean et al., 2008, Pitman et al., 2011, Awasaki et al., 2011, Donlea et al., 2009, Potter and Luo, 2010, Tanaka et al., 2008, Tamura et al., 2010, Pauls et al., 2010, Michels et al., 2011)
Alleles and Phenotypes
Causes alleles	Scer\GAL4^NP0225 (Tanaka et al., 2004, Masuda-Nakagawa et al., 2005, Wang et al., 2006, Grosjean et al., 2008, Pitman et al., 2011, Awasaki et al., 2011, Donlea et al., 2009, Potter and Luo, 2010, Tanaka et al., 2008, Tamura et al., 2010, Pauls et al., 2010, Michels et al., 2011)

Lethality References
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS) Stage Tissue/Position (including subcellular localization) Reference larval stage adult antennal lobe projection neuron \| subset (Masuda-Nakagawa et al., 2005) larval MB calyx glomerulus A1 (Masuda-Nakagawa et al., 2005) larval MB calyx glomerulus A2 (Masuda-Nakagawa et al., 2005) larval MB calyx glomerulus A3 (Masuda-Nakagawa et al., 2005) larval MB calyx glomerulus A4 (Masuda-Nakagawa et al., 2005) larval MB calyx glomerulus D4 (Masuda-Nakagawa et al., 2005) larval MB calyx glomerulus D1 (Masuda-Nakagawa et al., 2005) larval MB calyx glomerulus D5 (Masuda-Nakagawa et al., 2005) larval MB calyx glomerulus I2 (Masuda-Nakagawa et al., 2005) larval MB calyx glomerulus I4 (Masuda-Nakagawa et al., 2005) larval MB calyx glomerulus L1 (Masuda-Nakagawa et al., 2005) larval MB calyx glomerulus L2 (Masuda-Nakagawa et al., 2005) larval MB calyx glomerulus L6 (Masuda-Nakagawa et al., 2005) larval MB calyx glomerulus L8 (Masuda-Nakagawa et al., 2005) larval MB calyx glomerulus M2 (Masuda-Nakagawa et al., 2005) larval MB calyx glomerulus V5 (Masuda-Nakagawa et al., 2005) adult stage adult antennal lobe projection neuron \| subset (Tanaka et al., 2004, Potter and Luo, 2010) adult antennal lobe projection neuron (Tanaka et al., 2008) calyx of adult mushroom body (Tanaka et al., 2008) inner antenno-cerebral tract (Tanaka et al., 2008) lateral horn \| restricted (Potter and Luo, 2010) adult antennal lobe glomerulus \| subset (Tanaka et al., 2004)
Additional Information
Statement Reference Expression is observed in 67-73 projection neurons in 35 of 43 antennal glomeruli. (Tanaka et al., 2004)

Marker for	adult antennal lobe projection neuron (Tanaka et al., 2008) antennal glomerulus \| subset (Tanaka et al., 2004) relay interneuron \| subset (Tanaka et al., 2004)
Reflects expression of
Reporter construct used in assay	Avic\GFP^{S65T.Scer\UAS} (Tanaka et al., 2008) Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} (Tanaka et al., 2008, Potter and Luo, 2010) n-syb^{Scer\UAS.cEa.T:Avic\GFP} (Masuda-Nakagawa et al., 2005)
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Kyoto	112095 w^*; P{GawB}NP0225 / CyO, P{UAS-lacZ.UW14}UW14
Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	GAL4-NP225 (Wang et al., 2006) NP225 (Masuda-Nakagawa et al., 2005, Tanaka et al., 2004, Tamura et al., 2010, Pitman et al., 2011, Donlea et al., 2009, Tanaka et al., 2008, Awasaki et al., 2011, Pauls et al., 2010) NP225Gal4 (Grosjean et al., 2008) NP225-GAL4 (Potter and Luo, 2010) NP225-Gal4 (Michels et al., 2011) P{GawB}NP225 (Tanaka et al., 2004, FlyBase, 1992-) P{GawB}NP0225 (FlyBase, 1992-, )
Secondary FlyBase IDs

References ( 14 )
Research paper	Michels et al., 2011, Learn. Mem. 18(5): 332--344 Cellular site and molecular mode of synapsin action in associative learning. [FBrf0213538] Pitman et al., 2011, Curr. Biol. 21(10): 855--861 A pair of inhibitory neurons are required to sustain labile memory in the Drosophila mushroom body. [FBrf0213725] Potter and Luo, 2010, PLoS ONE 5(4): e10168 Splinkerette PCR for mapping transposable elements in Drosophila. [FBrf0210616] Tamura et al., 2010, J. Neurosci. 30(42): 14091--14101 Drosophila PQBP1 regulates learning acquisition at projection neurons in aversive olfactory conditioning. [FBrf0212135] Donlea et al., 2009, Science 324(5923): 105--108 Use-dependent plasticity in clock neurons regulates sleep need in Drosophila. [FBrf0207700] Grosjean et al., 2008, Nat. Neurosci. 11(1): 54--61 A glial amino-acid transporter controls synapse strength and courtship in Drosophila. [FBrf0201004] Tanaka et al., 2008, J. Comp. Neurol. 508(5): 711--755 Neuronal assemblies of the Drosophila mushroom body. [FBrf0205263] Wang et al., 2006, Development 133(7): 1231--1240 Steroid hormone-dependent transformation of polyhomeotic mutant neurons in the Drosophila brain. [FBrf0190330] Masuda-Nakagawa et al., 2005, Proc. Natl. Acad. Sci. U.S.A. 102(52): 19027--19032 Stereotypic and random patterns of connectivity in the larval mushroom body calyx of Drosophila. [FBrf0191451] Tanaka et al., 2004, Curr. Biol. 14(6): 449--457 Integration of chemosensory pathways in the Drosophila second-order olfactory centers. [FBrf0174482] Brand and Perrimon, 1993, Development 118(2): 401--415 Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. [FBrf0064375]
Supplementary material	Awasaki et al., 2011, Nat. Neurosci. 14(7): Supplementary information. [FBrf0217577] Pauls et al., 2010, J. Neurosci. 30(32): Supplemental Material. [FBrf0220210]
FlyBase analysis	FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]