Dmel\P{GSV1}sra^GS3168 Insertion

General Information
Symbol	Dmel\P{GSV1}sra^GS3168	Species	D. melanogaster
Name		FlyBase ID	FBti0064323
Feature type	transposable_element_insertion_site
Description
Inserted element	P{GSV1}	Expression data
Affected gene(s)	sra	Viability / fertility
Causes allele(s)	sra^GS3168	Stock availability	1 publicly available
LINE ID
Genomic Location
Chromosomal location	3R ( 89B7 )	Sequence location	3R:12,015,338..12,015,338
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	3R:12,015,338..12,015,338 (FlyBase, 2009.6.5)
Orientation
Cytological location (computed by FlyBase)	89B7 ( inferred by FlyBase from sequence location )
Cytological location (reported)	89B7 (reported as inferred from sequence location) (Muramatsu et al., 2007.6.13)
Comments concerning location
Sequence Data
Flanking sequence	AB309307 (Muramatsu et al., 2007.6.13) AB309308 (Muramatsu et al., 2007.6.13)
Inserted Element
Construct	P{GSV1}
Location-dependent role	mobile activating element (UASG) (Toba et al., 1999)
Size	5.284Kb
Associated alleles	Scer\UAS^5x.hs w^+mGS
Molecular map
Affected Gene(s)
Insertion may affect gene	sra (Ejima et al., 2004)
Alleles and Phenotypes
Causes alleles	sra^GS3168 (Ejima et al., 2004, Takeo et al., 2006)

Lethality References
Sterility References female sterile \| recessive (Ejima et al., 2004)
Phenotype Manifest In

Detailed Description
Statement Reference Expression of sra^GS3168 under the control of Scer\GAL4^elav-C155 or Scer\GAL4^sca-537.4 results in increased ovulation in virgin females compared to control virgin females. Within 30 minutes in a mating assay, only 35% of virgin females expressing sra^GS3168 under the control of Scer\GAL4^sca-537.4 have mated, compared to 82% of wild-type virgin females. Virgin females expressing sra^GS3168 under the control of Scer\GAL4^sca-537.4 elicit significantly lower courtship index (CI) levels (CI is the period of time within the observation period that a male displays any element of courtship behaviour) than wild-type virgin females. Virgin females expressing sra^GS3168 under the control of Scer\GAL4^sca-537.4 have a high extrusion index (EI, the percentage of a time a female shows extrusion behaviour when being courted by a male) which is nearly comparable to that of wild-type mated females. Homozygous females lay fewer eggs than wild type and most of the eggs are arrested at metaphase I of meiosis. Spontaneous ovulation occurs in 13% of mutant virgin females (virtually no ovulation occurs in wild-type virgin females). The level of ovulation of mutant mated females is not as high as that of wild-type mated females. The mutant females show reduced receptivity after mating (as occurs in wild-type females), but their remating rate 24 hours after mating is significantly higher than that of wild-type females. Receptivity of mutant females is restored earlier than for wild-type females; the remating rate is significantly higher for mutant females compared to wild-type females 10 days after mating. (Ejima et al., 2004)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Kyoto	200524 y¹ w^67c23; P{GSV1}sra^GS3168/TM3, Sb¹ Ser¹
Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{GS}sra^GS3168 (Ejima et al., 2004, FlyBase, 1992-) P{GSV1}sra^GS3168 (Toba et al., 1999)
Secondary FlyBase IDs

References ( 6 )
Research paper	Takeo et al., 2006, Curr. Biol. 16(14): 1435--1440 The calcineurin regulator Sra plays an essential role in female meiosis in Drosophila. [FBrf0194458] Ejima et al., 2004, Genetics 168(4): 2077--2087 Expression level of sarah, a homolog of DSCR1, is critical for ovulation and female courtship behavior in Drosophila melanogaster. [FBrf0182753] Toba et al., 1999, Genetics 151(2): 725--737 The gene search system. A method for efficient detection and rapid molecular identification of genes in Drosophila melanogaster. [FBrf0107369]
Personal communication to FlyBase	Muramatsu et al., 2007.6.13, GS vector insertion sites, flanking regions. GS vector insertion sites, flanking regions. [FBrf0200395]
FlyBase analysis	FlyBase, 2009.6.5, Mapping of GS vector insertion sites to genome release 5. Mapping of GS vector insertion sites to genome release 5. [FBrf0206797] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{GSV1}sraGS3168 Insertion

Dmel\P{GSV1}sra^GS3168 Insertion