|Inserted element||FB||Expression data|
|Affected gene(s)||Csk||Viability / fertility|
|Causes allele(s)||Cskj1D8||Stock availability||none publicly available|
|Chromosomal location||3R ( 86E13 )||Sequence location|
|Member of Large Scale Dataset(s)|
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|Detailed Mapping Data|
(computed by FlyBase)
86E13 ( near gene of known cytology )
|Alleles and Phenotypes|
lethal | pupal stage
|Phenotype Manifest In|
eye | somatic clone
eye-antennal disc | somatic clone
ommatidium | somatic clone | supernumerary
A Csk[j1D8]/+ background enhances the visual system degeneration seen in Scer\GAL4[GMR.PF], Ppt1[Scer\UAS.cKa] flies.
Csk[j1D8] dominantly suppresses the rough eye phenotype caused by expression of Zzzz\CTG[i480.Scer\UAS.cGa] under the control of Scer\GAL4[sev.PU].
Wing discs of homozygous third instar larvae show considerable apoptosis.
ASPP/ASPP Csk[j1D8]/+ adults show a notched wing phenotype. Wing discs of ASPP/ASPP Csk[j1D8]/+ third instar larvae show considerable apoptosis. Neither phenotype is seen in ASPP/ASPP or Csk[j1D8]/+ single mutants. Btk29A[k00206]/+ largely suppresses the ectopic apoptosis in wing discs and notched wing phenotype seen in ASPP/ASPP Csk[j1D8]/+ animals. puc[E69]/+ Csk[j1D8]/+ double heterozygotes have a notched wing phenotype, and third larval instar wing discs show ectopic apoptosis within the wing pouch. The ectopic apoptosis seen in the wing discs of puc[E69]/+ Csk[j1D8]/+ double heterozygotes is enhanced if they are also heterozygous for ASPP, resulting in adults with a severely reduced wing size. These adults also have leg deformities.
Cskj1D8, Stat92Ej6C8 mutant eyes are smaller than eyes mutant for Cskj1D8 or Stat92Ej6C8 alone. In addition, adult eyes are frequently fragmented, with scars and/or patches of eye tissue separated by patches of cuticle. Stat92E06346. Cskj1D8 mutant larval eye-antennal discs frequently show reduced sizes relative to the single mutants. Mutants also frequently show a reduction or absence of developing antennal tissues. Mutant larval eye tissue also contains many apoptotic cells.
Mutant larvae die within 6-8 hours after pupariation. The larval body mass of mutants increases up to 180% of the wild-type mass during the wandering stage. The brain ventral ganglion and salivary glands of mutants are enlarged. Pupariation is delayed. The body length of mutant pupae are about 121% the size of wild-type. Imaginal discs of mutant larvae are also larger than wild-type. The larval body mass of Cskj1D8/CskS030003 mutants increases up to 150% of the wild-type mass during the wandering stage. In the rare cases mutants survived to pharate adult stage, the head and eyes are enlarged. The posterior ommatidia are sometimes misaligned, though individual ommatidia are morphologically normal. Mutant eyes contain more ommatidia than wild-type. Rarely the eyes are replaced with duplicated antennae. In addition the wings and legs are severely malformed, the notum is sometimes split, and the head, legs and notum often contain cuticle overgrowths. In Cskj1D8/CskS030003 and homozygous Cskj1D8 mutant developing eye discs, an increase is seen in the number of mitotic cells in the morphogenetic furrow. Mutant pupal retinas contain excess mitotic cells in the interommatidial lattice. Cskj1D8/CskS030003 eye antennal disc cells exhibit a decrease in the proportion of cells in G0-G1 phase, and a consequent increase in cells in G2-M phase when compared to wild-type controls. Homozygous mutant clones in the eye produced severely overgrown eyes. Eyes have more than 1200 ommatidia, compared to about 687 in wild-type. Eyes can become malformed as a result. The extra ommatidia are occasionally inverted in their planar polarity but are otherwise normal in size and morphology. Occasionally mutant clones result in antennal duplication and cuticle overgrowth.
used in assay
|Data on Genetic Line|
|Origin as a multiple insertion line|
|Progenitor(s) within the Genome|
|Related Aberration or Balancer|
|Stocks ( 0 )|
|Synonyms & Secondary IDs|
|Secondary FlyBase IDs|
|References ( 11 )|
|Personal communication to FlyBase||