Dmel\FB{}Csk^j1D8 Insertion

General Information
Symbol	Dmel\FB{}Csk^j1D8	Species	D. melanogaster
Name		FlyBase ID	FBti0064511
Feature type	transposable_element_insertion_site
Description
Inserted element	FB	Expression data
Affected gene(s)	Csk	Viability / fertility
Causes allele(s)	Csk^j1D8	Stock availability	none publicly available
LINE ID	l(3)j1D8
Genomic Location
Chromosomal location	3R ( 86E13 )	Sequence location
Member of Large Scale Dataset(s)
Dataset
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)	86E13 ( near gene of known cytology )
Cytological location (reported)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	FB
Location-dependent role
Size
Associated alleles
Molecular map
Affected Gene(s)
Insertion may affect gene	Csk (Stewart et al., 2003)
Alleles and Phenotypes
Causes alleles	Csk^j1D8 (Stewart et al., 2003)

Lethality References lethal \| pupal stage (Read et al., 2004) lethal \| pupal stage \| recessive (Read et al., 2004, Langton et al., 2007) lethal \| recessive (Spradling et al., 1999, BDGP Project Members, 1994-1999)
Sterility References
Phenotype Manifest In
	eye \| somatic clone (Read et al., 2004) eye-antennal disc \| somatic clone (Read et al., 2004) ommatidium \| somatic clone \| supernumerary (Read et al., 2004) wing disc (Langton et al., 2007)
Detailed Description
Statement Reference A Csk[j1D8]/+ background enhances the visual system degeneration seen in Scer\GAL4[GMR.PF], Ppt1[Scer\UAS.cKa] flies. (Saja et al., 2010) 68% of ASPP[d] ; Csk[j1D8]/+ flies have notched wings. (Langton et al., 2009) Csk[j1D8] dominantly suppresses the rough eye phenotype caused by expression of Zzzz\CTG[i480.Scer\UAS.cGa] under the control of Scer\GAL4[sev.PU]. (Garcia-Lopez et al., 2008) Wing discs of homozygous third instar larvae show considerable apoptosis. (Langton et al., 2007) ASPP[8]/ASPP[8] Csk[j1D8]/+ adults show a notched wing phenotype. Wing discs of ASPP[8]/ASPP[8] Csk[j1D8]/+ third instar larvae show considerable apoptosis. Neither phenotype is seen in ASPP[8]/ASPP[8] or Csk[j1D8]/+ single mutants. Btk29A[k00206]/+ largely suppresses the ectopic apoptosis in wing discs and notched wing phenotype seen in ASPP[8]/ASPP[8] Csk[j1D8]/+ animals. puc[E69]/+ Csk[j1D8]/+ double heterozygotes have a notched wing phenotype, and third larval instar wing discs show ectopic apoptosis within the wing pouch. The ectopic apoptosis seen in the wing discs of puc[E69]/+ Csk[j1D8]/+ double heterozygotes is enhanced if they are also heterozygous for ASPP[8], resulting in adults with a severely reduced wing size. These adults also have leg deformities. (Langton et al., 2007) Csk^j1D8, Stat92E^j6C8 mutant eyes are smaller than eyes mutant for Csk^j1D8 or Stat92E^j6C8 alone. In addition, adult eyes are frequently fragmented, with scars and/or patches of eye tissue separated by patches of cuticle. Stat92E⁰⁶³⁴⁶. Csk^j1D8 mutant larval eye-antennal discs frequently show reduced sizes relative to the single mutants. Mutants also frequently show a reduction or absence of developing antennal tissues. Mutant larval eye tissue also contains many apoptotic cells. (Read et al., 2004) Mutant larvae die within 6-8 hours after pupariation. The larval body mass of mutants increases up to 180% of the wild-type mass during the wandering stage. The brain ventral ganglion and salivary glands of mutants are enlarged. Pupariation is delayed. The body length of mutant pupae are about 121% the size of wild-type. Imaginal discs of mutant larvae are also larger than wild-type. The larval body mass of Csk^j1D8/Csk^S030003 mutants increases up to 150% of the wild-type mass during the wandering stage. In the rare cases mutants survived to pharate adult stage, the head and eyes are enlarged. The posterior ommatidia are sometimes misaligned, though individual ommatidia are morphologically normal. Mutant eyes contain more ommatidia than wild-type. Rarely the eyes are replaced with duplicated antennae. In addition the wings and legs are severely malformed, the notum is sometimes split, and the head, legs and notum often contain cuticle overgrowths. In Csk^j1D8/Csk^S030003 and homozygous Csk^j1D8 mutant developing eye discs, an increase is seen in the number of mitotic cells in the morphogenetic furrow. Mutant pupal retinas contain excess mitotic cells in the interommatidial lattice. Csk^j1D8/Csk^S030003 eye antennal disc cells exhibit a decrease in the proportion of cells in G₀-G₁ phase, and a consequent increase in cells in G₂-M phase when compared to wild-type controls. Homozygous mutant clones in the eye produced severely overgrown eyes. Eyes have more than 1200 ommatidia, compared to about 687 in wild-type. Eyes can become malformed as a result. The extra ommatidia are occasionally inverted in their planar polarity but are otherwise normal in size and morphology. Occasionally mutant clones result in antennal duplication and cuticle overgrowth. (Read et al., 2004)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(3)j1D8 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 0 )

Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	FB{}Csk^j1D8 (Stewart et al., 2003, FlyBase, 1992-)
Secondary FlyBase IDs

References ( 11 )
Research paper	Saja et al., 2010, Neurobiol. Disease 40(1): 135--145 Identifying cellular pathways modulated by Drosophila palmitoyl-protein thioesterase 1 function. [FBrf0211567] Langton et al., 2009, Curr. Biol. 19(23): 1969--1978 The dASPP-dRASSF8 complex regulates cell-cell adhesion during drosophila retinal morphogenesis. [FBrf0209661] Garcia-Lopez et al., 2008, PLoS ONE 3(2): e1595 Genetic and chemical modifiers of a CUG toxicity model in Drosophila. [FBrf0210239] Langton et al., 2007, Dev. Cell 13(6): 773--782 Drosophila ASPP regulates C-terminal Src kinase activity. [FBrf0202129] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Read et al., 2004, Mol. Cell. Biol. 24(15): 6676--6689 Drosophila C-terminal Src kinase negatively regulates organ growth and cell proliferation through inhibition of the Src, Jun N-terminal kinase, and STAT pathways. [FBrf0179404] Stewart et al., 2003, Oncogene 22(41): 6436--6444 A genetic screen for modifiers of the lats tumor suppressor gene identifies C-terminal Src kinase as a regulator of cell proliferation in Drosophila. [FBrf0162272] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\FB{}Cskj1D8 Insertion

Dmel\FB{}Csk^j1D8 Insertion