A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{XP}CG33523d03153 Insertion

General Information
Symbol Dmel\P{XP}CG33523d03153 Species D. melanogaster
Name FlyBase ID FBti0068838
Feature type transposable_element_insertion_site
Description
Inserted element P{XP} Expression data
Affected gene(s) CG33523 Viability / fertility
Causes allele(s) CG33523d03153 Stock availability none publicly available
LINE ID d03153
Genomic Location
Chromosomal location 3L ( 65A1 ) Sequence location 3L:5,919,744..5,919,744 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBti0020044 FBti0113961 FBti0041682 FBti0146082 FBti0109580 FBti0036030 FBti0068838 FBti0054142 FBti0025174 FBti0068606 FBti0055275 FBti0070029 FBti0011648 FBti0044389 FBti0116070 FBti0045370 FBti0011737 FBti0011738 FBti0045179 FBti0036044 FBti0011723 FBti0059575 FBti0039313 FBti0110891
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{XP}. The P{XP} construct carries the w[+mC] mini-white marker, long (199-bp) Scer\FRT sites, and is designed to allow flexible use of Scer\UAS sites for Scer\GAL4-driven misexpression of adjacent genes. FRT sites allow Scer\FLP-mediated recombination between other FRT-containing elements, and thus can be used to generate molecularly defined deletions.
P{XP} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Thibault et al., 2004, Bellen et al., 2011)
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
3L:5,919,744..5,919,744 [-]
(Gene Disruption Project members and Exelixis, 2005)
Orientation
Cytological location
(computed by FlyBase)
65A1 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
65A1 (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
Comments concerning
location
hide Sequence Data
Flanking sequence
CZ473204
(Gene Disruption Project members, 2001-)
hide Inserted Element
Construct P{XP}
Location-dependent
role
deletion generation component
(Thibault et al., 2004)
mobile activating element
(Thibault et al., 2004)
carries FRT site(s)
(Thibault et al., 2004)
Size 7.303Kb
Associated alleles
gypsy\su(Hw)BR348
Scer\UAS14x.hs.XP.Scer\FRT
Scer\UAS14x.hs.XP
TrlBRcRa
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
CG33523
(FlyBase Curators, 2008)
hide Alleles and Phenotypes
Causes alleles
CG33523d03153
(FlyBase Curators, 2008)
Lethality
References
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
d03153
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 0 )
hide Linkouts
hide Comments
Location 3L:5900591-5900592 confirmed by FlyBase alignment of dbGSS accession CZ473204 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{XP}CG33523d03153
 
P{XP}d03153
(FlyBase, 2005, FlyBase, 1992-)
Secondary FlyBase IDs
hide References ( 10 )
Research paper
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Thibault et al., 2004, Nat. Genet. 36(3): 283--287
A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Personal communication to FlyBase
Christensen et al., 2008.4.15, Isolation and characterization of Df(3L)BSC437.
Isolation and characterization of Df(3L)BSC437. [FBrf0204472]
Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. (Computer file)
Genomic mapping of Exelixis insertion collection. (Computer file) [FBrf0184340]
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
FlyBase analysis
FlyBase Curators, 2013, Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}.
Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. [FBrf0221061]
FlyBase Curators, 2008, Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations.
Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. [FBrf0202955]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]