Dmel\P{EPgy2}CTCF^EY15833 Insertion

General Information
Symbol	Dmel\P{EPgy2}CTCF^EY15833	Species	D. melanogaster
Name		FlyBase ID	FBti0070849
Feature type	transposable_element_insertion_site
Description
Inserted element	P{EPgy2}	Expression data
Affected gene(s)	CTCF	Viability / fertility	fertile, viable
Causes allele(s)	CTCF^EY15833	Stock availability	1 publicly available
LINE ID	EY15833
Genomic Location
Chromosomal location	3L ( 65F6 )	Sequence location	3L:7,349,762..7,349,762
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{EPgy2}.EY A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EPgy2}; created and vetted by the Gene Disruption Project (GDP). The P{EPgy2} construct carries two visible markers, the mini-white marker w[+mC] and the mini-yellow marker y[+mDint2], and Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity. Additional P{EPgy2} insertion lines were generated, mapped, and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank. (Bellen et al., 2004, Bellen et al., 2011)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	3L:7,349,762..7,349,762 (Gene Disruption Project members, 2001-)
Orientation
Cytological location (computed by FlyBase)	65F6 ( inferred by FlyBase from sequence location )
Cytological location (reported)	65F6 (reported as inferred from sequence location) (Gene Disruption Project members, 2001-)
Comments concerning location
Sequence Data
Flanking sequence	CW627858 (Gene Disruption Project members, 2001-)
Inserted Element
Construct	P{EPgy2}
Location-dependent role	mobile activating element (UASG) (Bellen et al., 2004)
Size	10.908Kb
Associated alleles	Scer\UAS^14x.P\T TrlBR^cRa w^+mC y^+mDint2
Molecular map
Affected Gene(s)
Insertion may affect gene	CTCF (Gene Disruption Project members, 2001-, Mohan et al., 2007, Gerasimova et al., 2007)
Alleles and Phenotypes
Causes alleles	CTCF^EY15833 (Gene Disruption Project members, 2001-, Mohan et al., 2007, Gerasimova et al., 2007)

Lethality References viable (Gene Disruption Project members, 2001-, Macdonald et al., 2010)
Sterility References fertile (Gene Disruption Project members, 2001-, Macdonald et al., 2010)
Phenotype Manifest In

Detailed Description
Statement Reference Homozygous adults appear healthy and are reasonably fertile. Heterozygosity for CTCF[EY15833] substantially reduces the maternal, but not paternal, imprint of the g gene on the Dp(1;f)LJ9 mini-X chromosome. (Macdonald et al., 2010) CTCF[EY15833] mutants are viable and lack any visible phenotype. (Gerasimova et al., 2007) CTCF[EY15833] homozygotes and CTCF[EY15833]/Df(3L)0463 appear as wild-type. (Mohan et al., 2007)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	EY15833 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	21162 y¹ w^67c23; P{EPgy2}CTCF^EY15833
Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{EPgy2}CTCF^EY15833 (FlyBase, 1992-, Gerasimova et al., 2007, Mohan et al., 2007, ) P{EPgy2}EY15833 (FlyBase, 1992-)
Secondary FlyBase IDs

References ( 9 )
Research paper	Bellen et al., 2011, Genetics 188(3): 731--743 The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229] Macdonald et al., 2010, BMC Biol. 8(1): 105 The Drosophila homolog of the mammalian imprint regulator, CTCF, maintains the maternal genomic imprint in Drosophila melanogaster. [FBrf0211494] Gerasimova et al., 2007, Mol. Cell 28(5): 761--772 Coordinated control of dCTCF and gypsy chromatin insulators in Drosophila. [FBrf0201197] Mohan et al., 2007, EMBO J. 26(19): 4203--4214 The Drosophila insulator proteins CTCF and CP190 link enhancer blocking to body patterning. [FBrf0200449] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Personal communication to FlyBase	Meller and Lloyd, 2010.12.13, CTCF[30] map. CTCF[30] map. [FBrf0212748] Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177]
FlyBase analysis	FlyBase Curators, 2013, Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}. Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}. [FBrf0220667] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{EPgy2}CTCFEY15833 Insertion

Dmel\P{EPgy2}CTCF^EY15833 Insertion