Dmel\P{lacW}vih¹ Insertion

General Information
Symbol	Dmel\P{lacW}vih¹	Species	D. melanogaster
Name		FlyBase ID	FBti0072083
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	vih	Viability / fertility
Causes allele(s)	vih¹	Stock availability	none publicly available
LINE ID
Genomic Location
Chromosomal location	3L ( 69C4 )	Sequence location
Member of Large Scale Dataset(s)
Dataset
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)	69C4 ( near gene of known cytology )
Cytological location (reported)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	vih (Mathe et al., 2004)
Alleles and Phenotypes
Causes alleles	vih¹ (Mathe et al., 2004)

Lethality References lethal \| embryonic stage \| maternal effect \| recessive (Mathe et al., 2004) lethal \| partially \| pharate adult stage \| recessive (Mathe et al., 2004)
Sterility References
Phenotype Manifest In
	mitosis & nuclear chromosome (Mathe et al., 2004) mitosis & nuclear chromosome \| maternal effect (Mathe et al., 2004)
Detailed Description
Statement Reference Approximately 20% of mutants die as pharate adults. The mitotic index and proportion of cells at different phases of the mitotic cycle in the brains of homozygous larvae are not significantly different from those of wild type. However, the mutant brains have aberrant mitotic figures in which the chromosomes are scattered apparently randomly through the cell, and there is a significant increase in polyploid mitotic figures compared to wild type. The proportion of these defects are increased in hemizygous larvae compared to homozygous larvae. The mitotic chromosomes are highly condensed in metaphase and anaphase in vih¹ larvae. Spindle poles that lack centrosomes are seen and they are characteristically broad. Homozygous and hemizygous females produce syncytial embryos that fail to develop as a result of mitotic defects; the embryos rarely develop beyond the third nuclear division cycle, and mitotic figures have abnormal mitotic spindles and frequently have a polyploid complement of chromosomes. (Mathe et al., 2004)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 0 )

Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lacW}vih¹ (Mathe et al., 2004, FlyBase, 1992-)
Secondary FlyBase IDs

References ( 3 )
Research paper	Mathe et al., 2004, Curr. Biol. 14(19): 1723--1733 The E2-C vihar is required for the correct spatiotemporal proteolysis of cyclin B and itself undergoes cyclical degradation. [FBrf0180066] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
FlyBase analysis	FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{lacW}vih1 Insertion

Dmel\P{lacW}vih¹ Insertion