Dmel\P{EP}spas^T32 Insertion

General Information
Symbol	Dmel\P{EP}spas^T32	Species	D. melanogaster
Name		FlyBase ID	FBti0072106
Feature type	transposable_element_insertion_site
Description
Inserted element	P{EP}	Expression data
Affected gene(s)	spas	Viability / fertility
Causes allele(s)	spas^T32	Stock availability	none publicly available
LINE ID
Genomic Location
Chromosomal location	3R ( 95D9 )	Sequence location
Member of Large Scale Dataset(s)
Dataset
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)	95D9 ( near gene of known cytology )
Cytological location (reported)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{EP}
Location-dependent role	mobile activating element (UASG) (Rorth, 1996)
Size	7.987Kb
Associated alleles	Scer\UAS^14x.hs TrlBR^cRa w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	spas (Sherwood et al., 2004, Taniguchi et al., 2007, Ye et al., 2011)
Alleles and Phenotypes
Causes alleles	spas^T32 (Sherwood et al., 2004, Taniguchi et al., 2007, Ye et al., 2011)

Lethality References
Sterility References
Phenotype Manifest In
	dendritic arborizing neuron \| somatic clone (Ye et al., 2011) ventral nerve cord (Sherwood et al., 2004)
Detailed Description
Statement Reference spas[T32] mutant overexpression (through Scer\GAL4[ppk.PG]) leads to a dramatic reduction in both total dendritic length and branch number. Similarly, the number of branch points are reduced. spas upregulation by expression of spas[T32] results in a mild yet significant reduction in axonal growth in class IV da neurons. (Ye et al., 2011) Overexpression of spas^T32 in the hindgut epithelium (under the control of Scer\GAL4^NP2432) does not affect the laterality of this organ. (Taniguchi et al., 2007) When spas^T32 is driven by Scer\GAL4^sca-537.4, at 23^oC, mutant embryos embryos display abnormal midline crossing of the inner 1D4 bundle, and the entire ventral nerve cord (VNC) is narrowed at these crossing sites. At 29^oC the VNC collapses onto the midline and discrete longitudinal bundles are no longer apparent. (Sherwood et al., 2004)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 0 )

Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	OE Spa^T32 (Ye et al., 2011) P{EP}spas^T32 (Sherwood et al., 2004, FlyBase, 1992-) P{EP}T32 (Taniguchi et al., 2007) T32 (Ye et al., 2011) UAS-Spastin (Ye et al., 2011)
Secondary FlyBase IDs

References ( 5 )
Research paper	Ye et al., 2011, J. Neurosci. 31(9): 3309--3319 Differential regulation of dendritic and axonal development by the novel kruppel-like factor dar1. [FBrf0213162] Taniguchi et al., 2007, Fly 1(5): 287--290 Roles of type I myosins in Drosophila handedness. [FBrf0205051] Sherwood et al., 2004, PLoS Biol. 2(12): e429 Drosophila Spastin Regulates Synaptic Microtubule Networks and Is Required for Normal Motor Function. [FBrf0180480] Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422 A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
FlyBase analysis	FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{EP}spasT32 Insertion

Dmel\P{EP}spas^T32 Insertion