A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{EPg}ensHP36480 Insertion

General Information
Symbol Dmel\P{EPg}ensHP36480 Species D. melanogaster
Name FlyBase ID FBti0074891
Feature type transposable_element_insertion_site
Description
Inserted element P{EPg} Expression data
Affected gene(s) ens Viability / fertility
Causes allele(s) ensHP36480 Stock availability 1 publicly available
LINE ID HP36480
Genomic Location
Chromosomal location 3L ( 64A7 ) Sequence location 3L:4,133,932..4,133,932 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0146276 FBti0042634 FBti0074891 FBti0054933 FBti0102350 FBti0028584 FBti0107798 FBti0035967 FBti0100375 FBti0106627 FBti0106445 FBti0115531 FBti0077040 FBti0066300 FBti0045674 FBti0075590
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EPg}. The P{EPg} construct carries the mini-white marker w[+mC], and Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator (FBrf0128569). The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.
Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.
(Staudt et al., 2005, Bellen et al., 2011)
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
3L
(FlyBase, 1992-)
Sequence Location
3L:4,133,932..4,133,932 [+]
(Levis, 2009.8.13)
Orientation
+
(Levis, 2009.8.13)
Cytological location
(computed by FlyBase)
64A7 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
Comments concerning
location
The location specified is that of the genomic sequence nucleotide that aligns with the 5' end of the flanking sequence deposited by Staudt et al. (2005) in GenBank. The GenBank records do not specify the position of the insertion within the flanking sequence so there is ambiguity in the insertion position.
(Levis, 2009.8.13)
hide Sequence Data
Flanking sequence
AM270924
(FlyBase, 1992-)
hide Inserted Element
Construct P{EPg}
Location-dependent
role
mobile activating element (UASG)
(Mata et al., 2000)
Size 8.057Kb
Associated alleles
Scer\UAS14x.P\T
TrlBRcRa
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
ens
(Matthews, 2008.4.15)
hide Alleles and Phenotypes
Causes alleles
ensHP36480
(Matthews, 2008.4.15)
Lethality
References
Sterility
References
hide Phenotype Manifest In
lateral transverse muscle
(Metzger et al., 2012)
hide Detailed Description
Statement
Reference
ens[HP36480] fails to complement the myonuclear positioning defect of ens[swo].
(Metzger et al., 2012)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
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FlyView (LinkOut)
hide Data on Genetic Line
Line ID
HP36480
(FlyBase, 1992-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
21996
w1118; P{EPg}ensHP36480
hide Linkouts
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
HP36480
(FlyBase, 1992-)
P{EPg}CG14998HP36480
(FlyBase, 1992-, Matthews, 2008.4.15)
P{EPg}ensHP36480
 
P{EPg}HP36480
(FlyBase, 1992-)
Secondary FlyBase IDs
hide References ( 9 )
Research paper
Metzger et al., 2012, Nature 484(7392): 120--124
MAP and kinesin-dependent nuclear positioning is required for skeletal muscle function. [FBrf0218014]
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Staudt et al., 2005, PLoS Genet. 1(4): e55
Gain-of-function screen for genes that affect Drosophila muscle pattern formation. [FBrf0191391]
Mata et al., 2000, Cell 101(5): 511--522
Tribbles coordinates mitosis and morphogenesis in Drosophila by regulating string/CDC25 proteolysis. [FBrf0128569]
Supplementary material
Staudt et al., 2005, PLoS Genet. 1(4):
Table S2. [FBrf0208297]
Personal communication to FlyBase
Levis, 2009.8.13, Release 5 sequence localizations for HP and DP lines.
Release 5 sequence localizations for HP and DP lines. [FBrf0208533]
Matthews, 2008.4.15, DP and HP P-element insertions submitted to the Bloomington Drosophila Stock Center.
DP and HP P-element insertions submitted to the Bloomington Drosophila Stock Center. [FBrf0204789]
FlyBase analysis
FlyBase Curators, 2013, Members of TE insertion collections.
Members of TE insertion collections. [FBrf0220668]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]