Dmel\P{EPg}HP35866 Insertion

General Information
Symbol	Dmel\P{EPg}HP35866	Species	D. melanogaster
Name		FlyBase ID	FBti0074911
Feature type	transposable_element_insertion_site
Description
Inserted element	P{EPg}	Expression data
Affected gene(s)		Viability / fertility
Causes allele(s)		Stock availability	1 publicly available
LINE ID	HP35866
Genomic Location
Chromosomal location	3L ( 71E1 )	Sequence location	3L:15,573,600..15,573,600 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{EPg}.HP A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EPg}. The P{EPg} construct carries the mini-white marker w[+mC], and Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator (FBrf0128569). The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity. Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection. (Staudt et al., 2005, Bellen et al., 2011)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)	3L (FlyBase, 1992-)
Sequence Location	3L:15,573,600..15,573,600 [+] (Levis, 2009.8.13)
Orientation	+ (Levis, 2009.8.13)
Cytological location (computed by FlyBase)	71E1 ( inferred by FlyBase from sequence location )
Cytological location (reported)
Comments concerning location	The location specified is that of the genomic sequence nucleotide that aligns with the 5' end of the flanking sequence deposited by Staudt et al. (2005) in GenBank. The GenBank records do not specify the position of the insertion within the flanking sequence so there is ambiguity in the insertion position. (Levis, 2009.8.13)
Sequence Data
Flanking sequence	AM270904 (FlyBase, 1992-)
Inserted Element
Construct	P{EPg}
Location-dependent role	mobile activating element (UASG) (Mata et al., 2000)
Size	8.057Kb
Associated alleles	Scer\UAS^14x.P\T TrlBR^cRa w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene
Alleles and Phenotypes
Causes alleles

Lethality References
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	HP35866 (FlyBase, 1992-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	21988 w¹¹¹⁸; P{EPg}HP35866
Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	HP35866 (FlyBase, 1992-) P{EPg}HP35866 (FlyBase, 1992-)
Secondary FlyBase IDs

References ( 7 )
Research paper	Bellen et al., 2011, Genetics 188(3): 731--743 The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229] Staudt et al., 2005, PLoS Genet. 1(4): e55 Gain-of-function screen for genes that affect Drosophila muscle pattern formation. [FBrf0191391] Mata et al., 2000, Cell 101(5): 511--522 Tribbles coordinates mitosis and morphogenesis in Drosophila by regulating string/CDC25 proteolysis. [FBrf0128569]
Supplementary material	Staudt et al., 2005, PLoS Genet. 1(4): Table S2. [FBrf0208297]
Personal communication to FlyBase	Levis, 2009.8.13, Release 5 sequence localizations for HP and DP lines. Release 5 sequence localizations for HP and DP lines. [FBrf0208533]
FlyBase analysis	FlyBase Curators, 2013, Members of TE insertion collections. Members of TE insertion collections. [FBrf0220668] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]