Dmel\P{EPg}pex2^HP35039 Insertion

General Information
Symbol	Dmel\P{EPg}pex2^HP35039	Species	D. melanogaster
Name		FlyBase ID	FBti0074946
Feature type	transposable_element_insertion_site
Description
Inserted element	P{EPg}	Expression data
Affected gene(s)	pex2	Viability / fertility
Causes allele(s)	pex2^HP35039	Stock availability	1 publicly available
LINE ID	HP35039
Genomic Location
Chromosomal location	3L ( 66C7 )	Sequence location	3L:8,333,166..8,333,166 [-]
Map ( GBrowse )
Recent Updates
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FB2012_01
FB2011_10
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Detailed Mapping Data
Chromosome (arm)	3L (FlyBase, 1992-)
Sequence Location	3L:8,333,166..8,333,166 [-] (Levis, 2009.8.13)
Orientation	- (Levis, 2009.8.13)
Cytological location (computed by FlyBase)	66C7 ( inferred by FlyBase from sequence location )
Cytological location (reported)
Comments concerning location	The location specified is that of the genomic sequence nucleotide that aligns with the 5' end of the flanking sequence deposited by Staudt et al. (2005) in GenBank. The GenBank records do not specify the position of the insertion within the flanking sequence so there is ambiguity in the insertion position. (Levis, 2009.8.13)
Sequence Data
Flanking sequence	AM270869 (FlyBase, 1992-)
Inserted Element
Construct	P{EPg}
Location-dependent role	mobile activating element (UASG) (Mata et al., 2000)
Size	8.057Kb
Associated alleles	Scer\UAS^14x.P\T TrlBR^cRa w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	pex2 (Matthews, 2008.4.15)
Alleles and Phenotypes
Causes alleles	pex2^HP35039 (Matthews, 2008.4.15)

Lethality References viable (Chen et al., 2010)
Sterility References male sterile (Chen et al., 2010)
Phenotype Manifest In
	spermatid (Chen et al., 2010) testis (Chen et al., 2010)
Detailed Description
Statement Reference The male sterility found in pex2[HP35039] can be rescued through expression of pex2[Scer\UAS.cCa] under the control of Scer\GAL4[nos.PG] or Scer\GAL4[Hsp83.PA]. Expression under the control of Scer\GAL4[ptc-559.1] fails to rescue. The male sterility found in pex2[HP35039] can be rescued through expression of pex2[Scer\UAS.T:Disc\RFP-mCherry] under the control of Scer\GAL4[nos.PG] or Scer\GAL4[Hsp83.PA]. Expression under the control of Scer\GAL4[ptc-559.1] fails to rescue. (Chen et al., 2010) pex2[HP35039] mutant testes contain many spermatocytes and some \'cotton ball\'-like spheres but completely lack elongated spermatid bundles and mature sperm. The mutant testes appear much thinner and longer on the seventh day after eclosion than they do immediately after eclosion. pex2[HP35039] mutants display growth defects, with mutants exhibiting a 2-day delay in eclosion. pex2[HP35039] mutant testes do not contain hardly any normal mature spermatocytes. Instead, only a few fused spermatocytes with condensed chromosomes are found. pex2[HP35039] mutant testes do not contain hardly any normal mature spermatocytes. Instead, only a few fused spermatocytes with condensed chromosomes are found. in addition, there are more spermatocytes in pex2[HP35039] mutants than wild-type. In pex2[HP35039] mutants, cysts of 16 spermatocytes without cell-cell boundaries and with large round nuclei are present. This suggests that the spermatocyte growth stage is defective. The majority of spermatocytes do not mature, and some are fused. The fused spermatocytes are able to continue develop but exhibit profound defects in spermatid differentiation. Only a few round spermatids are found compared with wild-type and they often exhibit four or more nuclei associated with a single enlarged mitochondrial derivative, indicating a failure of cytokinesis during meiotic division. Chromosome segregation appears unaffected. Unlike wild-type testes, which have very long spermatid bundles, the few spermatids in pex2[HP35039] mutants, presumably from a cyst, form \'cotton ball\'-like spheres. Thus pex2[HP35039] mutant spermatids can initiate the extension of sperm tails, but this process is abnormal. pex2[HP35039] mutant spermatocytes go through G1/S phase normally but do not reach the G2/M transition. (Chen et al., 2010) A pex2[HP35039]/+ background partially suppresses the male sterility and cytokinesis defects found in bond[Z3437] mutants. (Chen et al., 2010)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	HP35039 (FlyBase, 1992-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	21973 w¹¹¹⁸; P{EPg}pex2^HP35039/TM3, Sb¹
Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	HP35039 (FlyBase, 1992-) P{EPg}CG7081^HP35039 (Matthews, 2008.4.15, FlyBase, 1992-, ) P{EPg}HP35039 (FlyBase, 1992-) P{EPg}pex2^HP35039
Secondary FlyBase IDs

References ( 7 )
Research paper	Chen et al., 2010, Hum. Mol. Genet. 19(3): 494--505 Drosophila models of peroxisomal biogenesis disorder: peroxins are required for spermatogenesis and very-long-chain fatty acid metabolism. [FBrf0209624] Staudt et al., 2005, PLoS Gen. 1(4): 499--506 Gain-of-function screen for genes that affect Drosophila muscle pattern formation. [FBrf0191391] Mata et al., 2000, Cell 101(5): 511--522 Tribbles coordinates mitosis and morphogenesis in Drosophila by regulating string/CDC25 proteolysis. [FBrf0128569]
Supplementary material	Staudt et al., 2005, PLoS Genet. 1(4): Table S2. [FBrf0208297]
Personal communication to FlyBase	Levis, 2009.8.13, Release 5 sequence localizations for HP and DP lines. Release 5 sequence localizations for HP and DP lines. [FBrf0208533] Matthews, 2008.4.15, DP and HP P-element insertions submitted to the Bloomington Drosophila Stock Center. DP and HP P-element insertions submitted to the Bloomington Drosophila Stock Center. [FBrf0204789]
FlyBase analysis	FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]

Dmel\P{EPg}pex2HP35039 Insertion

Dmel\P{EPg}pex2^HP35039 Insertion