A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{EPgy2}EY23609 Insertion

General Information
Symbol Dmel\P{EPgy2}EY23609 Species D. melanogaster
Name FlyBase ID FBti0075464
Feature type transposable_element_insertion_site
Description
Inserted element P{EPgy2} Expression data
Affected gene(s) Viability / fertility fertile, viable
Causes allele(s) Stock availability 1 publicly available
LINE ID EY23609
Genomic Location
Chromosomal location 2L ( 26F5 ) Sequence location 2L:6,656,407..6,656,407 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0023574 FBti0028297 FBti0034786 FBti0103640 FBti0036708 FBti0102672 FBti0102726 FBti0006415 FBti0107809 FBti0103875 FBti0075464 FBti0124610 FBti0115136 FBti0011267 FBti0124611_1 FBti0124611_2 FBti0023527 FBti0124612_1 FBti0124612_2 FBti0044786 FBti0047454
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EPgy2}; created and vetted by the Gene Disruption Project (GDP). The P{EPgy2} construct carries two visible markers, the mini-white marker w[+mC] and the mini-yellow marker y[+mDint2], and Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.
Additional P{EPgy2} insertion lines were generated, mapped, and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2L:6,656,407..6,656,407 [+]
Orientation
Cytological location
(computed by FlyBase)
26F5 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
26F5 (reported as inferred from sequence location)
Comments concerning
location
hide Sequence Data
Flanking sequence
hide Inserted Element
Construct P{EPgy2}
Location-dependent
role
mobile activating element (UASG)
Size 10.908Kb
Associated alleles
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
hide Alleles and Phenotypes
Causes alleles
Lethality
References
viable
Sterility
References
fertile
hide Phenotype Manifest In
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Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
EY23609
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
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hide Comments
LINECOMMENT:Probably separated before balancing from 2nd insertion in 36A
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{EPgy2}EY23609
Secondary FlyBase IDs
hide References ( 4 )
Research paper
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Personal communication to FlyBase
Bellen, 2007.2.7, batch722.xml
batch722.xml [FBrf0191800]
FlyBase analysis
FlyBase Curators, 2013, Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}.
Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}. [FBrf0220667]