Dmel\Mi{ET1}Gr64e^MB03533 Insertion

General Information
Symbol	Dmel\Mi{ET1}Gr64e^MB03533	Species	D. melanogaster
Name		FlyBase ID	FBti0075706
Feature type	transposable_element_insertion_site
Description
Inserted element	Mi{ET1}	Expression data
Affected gene(s)	Gr64d, Gr64e	Viability / fertility	fertile, viable
Causes allele(s)	Gr64d^MB03533, Gr64e^MB03533	Stock availability	1 publicly available
LINE ID	MB03533
Genomic Location
Chromosomal location	3L ( 64A4 )	Sequence location	3L:4,034,381..4,034,381 [-]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_Mi{ET1}.MB A set of transgenic insertion stocks derived by TE mobilization using the Dhyd\Minos-based construct Mi{ET1}; vetted by the Gene Disruption Project (GDP). The Mi{ET1} construct carries the Avic\GFP[E.3xP3] fluorescent marker, a Scer\GAL4 driver/reporter gene, and bacterial sequences that allow plasmid rescue. Additional Mi{ET1} insertion lines were generated, mapped, and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank. (Metaxakis et al., 2005, Bellen et al., 2011)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	3L:4,034,381..4,034,381 [-] (Bellen, 2007.4.5)
Orientation
Cytological location (computed by FlyBase)	64A4 ( inferred by FlyBase from sequence location )
Cytological location (reported)	64A4 (reported as inferred from sequence location) (Bellen, 2007.4.5)
Comments concerning location
Sequence Data
Flanking sequence	EI415323 (Bellen, 2007.4.5)
Inserted Element
Construct	Mi{ET1}
Location-dependent role	GAL4 driver/enhancer trap (Metaxakis et al., 2005)
Size	7.5, 7.5Kb
Associated alleles	Avic\GFP^E.3xP3 Scer\GAL4^ET1
Molecular map
Affected Gene(s)
Insertion may affect gene	Gr64d (Bellen, 2007.4.5) Gr64e (Bellen, 2007.4.5, Wisotsky et al., 2011, Jiao et al., 2008)
Alleles and Phenotypes
Causes alleles	Gr64d^MB03533 (Bellen, 2007.4.5) Gr64e^MB03533 (Bellen, 2007.4.5, Wisotsky et al., 2011, Jiao et al., 2008)

Lethality References viable (Bellen, 2007.4.5)
Sterility References fertile (Bellen, 2007.4.5)
Phenotype Manifest In

Detailed Description
Statement Reference Expression of Gr64e[Scer\UAS.cWa] in sugar taste neurons (under the control of Scer\GAL4[Gr5a.8.5]) restores glycerol sensitivity, although only partial rescue is seen at a 1% glycerol concentration. (Wisotsky et al., 2011) Gr64e[MB03533] mutants show a marked loss of preference for 30% beer against 5mM (as in wild-type). Gr64e[MB03533] flies show a substantially reduced preference for yeast extract and fermented grape. The preference for ripe banana is indistinguishable from wild-type flies. Gr64e[MB03533] mutant flies show virtually no response to 10% glycerol, whereas control flies show a strong response. Gr64e[MB03533] flies have a responsive sugar neuron, as a stimulus of 100mM sucrose elicits a firing rate comparable to that in control flies. L-type sensilla in Gr64e[MB03533] flies lack a response to 10% glycerol. In contrast, mean responses to sucrose, fructose and glucose are as in wild-type. Gr64e[MB03533] flies show greatly reduced proboscis extension responses to glycerol. In feeding preference assays in which populations of flies are presented with 5mM sucrose against varying concentrations of glycerol, wild-type flies show strong preferences for 1% and 10% glycerol. (Wisotsky et al., 2011)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	MB03533 (Bellen, 2007.4.5)
Origin as a multiple insertion line	N (Bellen, 2007.4.5)
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	23628 w¹¹¹⁸; Mi{ET1}Gr64e^MB03533 Gr64d^MB03533
Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	Gr64e^MB03533 (Wisotsky et al., 2011) MB03533 (Jiao et al., 2008) Mi{ET1}Gr64e^MB03533 (Bellen, 2007.4.5, )
Secondary FlyBase IDs

References ( 8 )
Research paper	Bellen et al., 2011, Genetics 188(3): 731--743 The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229] Wisotsky et al., 2011, Nat. Neurosci. 14(12): 1534--1541 Evolutionary differences in food preference rely on Gr64e, a receptor for glycerol. [FBrf0216771] Jiao et al., 2008, Curr. Biol. 18(22): 1797--1801 Gr64f is required in combination with other gustatory receptors for sugar detection in Drosophila. [FBrf0206328] Metaxakis et al., 2005, Genetics 171(2): 571--581 Minos as a genetic and genomic tool in Drosophila melanogaster. [FBrf0190750] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Personal communication to FlyBase	Bellen, 2007.4.5, batch731.xml batch731.xml [FBrf0191801]
FlyBase analysis	FlyBase Curators, 2013, Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}. Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}. [FBrf0220667] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\Mi{ET1}Gr64eMB03533 Insertion

Dmel\Mi{ET1}Gr64e^MB03533 Insertion