A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{EPgy2}βTub56DEY21785 Insertion

General Information
Symbol Dmel\P{EPgy2}βTub56DEY21785 Species D. melanogaster
Name FlyBase ID FBti0076010
Feature type transposable_element_insertion_site
Description
Inserted element P{EPgy2} Expression data
Affected gene(s) βTub56D Viability / fertility fertile, viable
Causes allele(s) βTub56DEY21785 Stock availability 1 publicly available
LINE ID EY21785
Genomic Location
Chromosomal location 2R ( 56D7 ) Sequence location 2R:15,337,649..15,337,649 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBti0006339 FBti0041314 FBti0105252 FBti0102325 FBti0115571 FBti0058320 FBti0025881 FBti0066774 FBti0041472 FBti0066556 FBti0052434 FBti0125300 FBti0106847 FBti0125301_2 FBti0125301_1 FBti0114984 FBti0025856 FBti0102822 FBti0106890 FBti0110374 FBti0076010 FBti0028719 FBti0006925 FBti0105004 FBti0071164 FBti0067070 FBti0011303 FBti0046521 FBti0056388 FBti0099882 FBti0067112 FBti0034062 FBti0057703 FBti0036930 FBti0023596 FBti0147299 FBti0125302 FBti0113045 FBti0112094 FBti0106323 FBti0059584 FBti0028718 FBti0025665 FBti0109934 FBti0039941 FBti0105983 FBti0007774 FBti0059583 FBti0106713 FBti0103398 FBti0006787_2 FBti0006787_1 FBti0072159 FBti0106079 FBti0109836 FBti0111372 FBti0111126 FBti0110307 FBti0113445 FBti0016284 FBti0104859 FBti0108426 FBti0045077 FBti0014543 FBti0125303_2 FBti0125303_1
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EPgy2}; created and vetted by the Gene Disruption Project (GDP). The P{EPgy2} construct carries two visible markers, the mini-white marker w[+mC] and the mini-yellow marker y[+mDint2], and Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.
Additional P{EPgy2} insertion lines were generated, mapped, and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2R:15,337,649..15,337,649 [-]
Orientation
Cytological location
(computed by FlyBase)
56D7 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
56D8 (reported as inferred from sequence location)
Comments concerning
location
hide Sequence Data
Flanking sequence
hide Inserted Element
Construct P{EPgy2}
Location-dependent
role
mobile activating element (UASG)
Size 10.908Kb
Associated alleles
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
hide Alleles and Phenotypes
Causes alleles
Lethality
References
viable
Sterility
References
fertile
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
EY21785
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
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hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{EPgy2}βTub56DEY21785
Secondary FlyBase IDs
hide References ( 4 )
Research paper
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Personal communication to FlyBase
Bellen, 2006.8.3, MBBatch686_2.xml
MBBatch686_2.xml [FBrf0191805]
FlyBase analysis
FlyBase Curators, 2013, Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}.
Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}. [FBrf0220667]