A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{EPgy2}Mob4EY23407 Insertion

General Information
Symbol Dmel\P{EPgy2}Mob4EY23407 Species D. melanogaster
Name FlyBase ID FBti0076142
Feature type transposable_element_insertion_site
Description
Inserted element P{EPgy2} Expression data
Affected gene(s) Mob4 Viability / fertility
Causes allele(s) Mob4EY23407 Stock availability 1 publicly available
LINE ID EY23407
Genomic Location
Chromosomal location 2R ( 42C5 ) Sequence location 2R:2,583,946..2,583,946 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBti0076142 FBti0023434 FBti0024490 FBti0104740 FBti0105699 FBti0037224 FBti0112376 FBti0109897 FBti0104427 FBti0039675 FBti0071042 FBti0099843 FBti0044238 FBti0051506 FBti0078443
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EPgy2}; created and vetted by the Gene Disruption Project (GDP). The P{EPgy2} construct carries two visible markers, the mini-white marker w[+mC] and the mini-yellow marker y[+mDint2], and Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.
Additional P{EPgy2} insertion lines were generated, mapped, and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Bellen et al., 2004, Bellen et al., 2011)
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2R:2,583,946..2,583,946 [-]
(Bellen, 2006.8.3)
Orientation
Cytological location
(computed by FlyBase)
42C5 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
42C5 (reported as inferred from sequence location)
(Bellen, 2006.8.3)
Comments concerning
location
hide Sequence Data
Flanking sequence
DX977913
(Bellen, 2006.8.3)
hide Inserted Element
Construct P{EPgy2}
Location-dependent
role
mobile activating element (UASG)
(Bellen et al., 2004)
Size 10.908Kb
Associated alleles
Scer\UAS14x.P\T
TrlBRcRa
w+mC
y+mDint2
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Mob4
(Bellen, 2006.8.3, Schulte et al., 2010)
hide Alleles and Phenotypes
Causes alleles
Mob4EY23407
(Bellen, 2006.8.3, Schulte et al., 2010)
Lethality
References
lethal
(Schulte et al., 2010)
lethal | recessive
(Schulte et al., 2010)
viable
(Schulte et al., 2010)
Sterility
References
hide Phenotype Manifest In
NMJ bouton | supernumerary
(Schulte et al., 2010)
hide Detailed Description
Statement
Reference
Approximately 25% of homozygotes develop to third instar larvae. Approximately 35% of Mob4[EY23407]/Df(2R)42 animals develop to third instar larvae, while approximately 10% pupate but do not eclose. 15% of Mob4[EYΔL3]/Mob4[EY23407] animals develop to the third larval instar, 3% pupate but do not eclose. Third instar mutant larvae have a "tail-flip" phenotype. Mob4[EY23407]/Df(2R)42 and Mob4[EYΔL3]/Mob4[EY23407] third instar larvae have supernumerary boutons at the neuromuscular junctions of muscles 6/7 and 4 compared to controls.
(Schulte et al., 2010)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
EY23407
(Bellen, 2006.8.3)
Origin as a multiple insertion line
N
(Bellen, 2006.8.3)
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
22632
y1 w67c23; P{EPgy2}Mob4EY23407/CyO
hide Linkouts
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
EY23407
(Schulte et al., 2010)
P{EPgy2}CG3403EY23407
(Bellen, 2006.8.3)
P{EPgy2}Mob4EY23407
 
Secondary FlyBase IDs
hide References ( 5 )
Research paper
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Schulte et al., 2010, J. Neurosci. 30(15): 5189--5203
DMob4/Phocein regulates synapse formation, axonal transport, and microtubule organization. [FBrf0210612]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Personal communication to FlyBase
Bellen, 2006.8.3, MBBatch686_2.xml
MBBatch686_2.xml [FBrf0191805]
FlyBase analysis
FlyBase Curators, 2013, Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}.
Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}. [FBrf0220667]