PBac{WH} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
A set of transgenic insertion stocks derived by TE mobilization using the Tni\piggyBac-based construct PBac{WH}. The PBac{WH} construct carries the w+mC mini-white marker, a single long (199-bp) Scer\FRT site, and a terminal Scer\UAS site for Scer\GAL4-driven misexpression of adjacent genes. The FRT site allows Scer\FLP-mediated recombination between other FRT-containing elements, and thus can be used to generate molecularly defined deletions.
Exelixis, Inc. determined the insertion site ofthe progenitor PBac{WH}f03562 to be at Release 3 genomic coordinate 14988104 on chromosome arm 2L. The Gene Disruption project determined the insertion site of the progenitor PBac{WH}f03562 to be at Release 3 genomic coordinate 14988091 on arm 2L. This corresponds to 35B8 on the Release 5 genome map.
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f03562