Dmel\P{EP}Hsrω^EP93D Insertion

General Information
Symbol	Dmel\P{EP}Hsrω^EP93D	Species	D. melanogaster
Name		FlyBase ID	FBti0076969
Feature type	transposable_element_insertion_site
Description
Inserted element	P{EP}	Expression data
Affected gene(s)	Hsrω	Viability / fertility	viable, fertile
Causes allele(s)	Hsrω^EP93D	Stock availability	none publicly available
LINE ID
Genomic Location
Chromosomal location	3R ( 93D4-93D5 )	Sequence location
Member of Large Scale Dataset(s)
Dataset	TI_set_P{EP}.EP A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EP}. The P{EP} construct construct carries a w[+mC] mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity. Insertion lines from this collection were mapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank. (Rorth, 1996, Bellen et al., 2004)
Recent Updates
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)	3 (Mallik and Lakhotia, 2009)
Sequence Location
Orientation
Cytological location (computed by FlyBase)	93D4-93D5 ( near gene of known cytology )
Cytological location (reported)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{EP}
Location-dependent role	mobile activating element (UASG) (Rorth, 1996)
Size	7.987Kb
Associated alleles	Scer\UAS^14x.hs TrlBR^cRa w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Hsrω (Sengupta and Lakhotia, 2006, Mallik and Lakhotia, 2009, Mallik and Lakhotia, 2009, Mallik and Lakhotia, 2010, Onorati et al., 2011)
Alleles and Phenotypes
Causes alleles	Hsrω^EP93D (Sengupta and Lakhotia, 2006, Mallik and Lakhotia, 2009, Mallik and Lakhotia, 2009, Mallik and Lakhotia, 2010, Onorati et al., 2011)

Lethality References viable (Mallik and Lakhotia, 2009)
Sterility References fertile (Mallik and Lakhotia, 2009) semi-fertile (Mallik and Lakhotia, 2009)
Phenotype Manifest In
	ommatidium (Sengupta and Lakhotia, 2006)
Detailed Description
Statement Reference Expression of Hsrω[EP93D] under the control of Scer\GAL4[Act5C.PI] enhances the polytene chromosome condensation defects of Iswi[unspecified] mutants. (Onorati et al., 2011) Expression of Hsrω[EP93D] in the Scer\GAL4[GMR.PF], Zzzz\CAG[127Q.Scer\UAS.T:Ivir\HA1], Df(3R)Hrb87F/+ background significantly enhances the appearence of black lesions on the eye surface, although it has no effect on mortality. Expression of nej[F2161A.Scer\UAS.T:SV5\V5] in the Scer\GAL4[GMR.PF], Zzzz\CAG[127Q.Scer\UAS.T:Ivir\HA1] background results in 23% lethality at the pupal stage. Surviving flies show a reduction in eye size and a greater disruption in ommatidial arrays. Additional co-expression of Hsrω[EP93D] results in a greater reduction in eye size, near complete loss of ommatidial arrays and bristles and the appearence of black lesions on the eye surface. Co-expression of Hsrω[EP93D] does not enhance pupal death. (Mallik and Lakhotia, 2010) Coexpression of one copy of Hsrω[EP93D] in the Scer\GAL4[GMR.PF], nej[F2161A.Scer\UAS.T:SV5\V5] background exaggerates the eye damage. Co-expression of Hsrω[EP93D] in the Scer\GAL4[GMR.PF], nej[dsRNA.Scer\UAS.cKa] background enhances eye degeneration with the loss of ommatidial integrity extending to most of the eye. Co-expression of Hsrω[EP93D] enhances the eye damage seen in Scer\GAL4[GMR.PF], nej[ΔNZK.Scer\UAS] flies. (Mallik and Lakhotia, 2010) Co-expression of Hsrω[EP93D] significantly enhances the Zzzz\CAG[127Q.Scer\UAS.T:Ivir\HA1], Scer\GAL4[GMR.PF] eye phenotype, leading to extensive black lesions on the eye surface. Co-expression of a single copy of P{Sym-UAS-Hsrω} eliminates the enhancing effect of Hsrω[EP93D] expression on the Zzzz\CAG[127Q.Scer\UAS.T:Ivir\HA1], Scer\GAL4[GMR.PF] eye phenotype, and also substantially reverses the eye degeneration primarily induced by Zzzz\CAG[127Q.Scer\UAS.T:Ivir\HA1]. (Mallik and Lakhotia, 2009) Expression of two copies of Hsrω[EP93D] enhances the rough eye, photoreceptor degeneration, and increased apoptosis phenotypes seen in Scer\GAL4[GMR.PF]/Scer\GAL4[GMR.PF] flies. Co-expression of Hsrω[EP93D] leads to a slight enhancement of the eye phenotype in Scer\GAL4[GMR.PF],th[dsRNA.Scer\UAS.cLa] flies. Co-expression of Hsrω[EP93D] suppresses the pupal lethality of Scer\GAL4[GMR.PF],th[dsRNA.Scer\UAS.cLa] flies. Coexpression of Hsrω[EP93D] enhances the Scer\GAL4[GMR.PF], egr[Scer\UAS.cIa] eye phenotype, leading to severe head malformations and the formation of black lesions on the eye, though pupal lethality is not increased. Coexpression of Hsrω[EP93D] enhances the Scer\GAL4[GMR.PF], Tak1[Scer\UAS.cTa] eye phenotype, though pupal lethality is not increased. (Mallik and Lakhotia, 2009) Eyes of Hsrω^EP93D homozygous flies show disorganisation of ommatidial units. (Sengupta and Lakhotia, 2006) The neurodegeneration phenotype caused by expression of Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR.PF is enhanced by co-expression of Hsrω^EP93D under the control of Scer\GAL4^GMR.PF; extensive black necrotic lesions are seen in the eye. (Sengupta and Lakhotia, 2006)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 0 )

Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	EP93D (Onorati et al., 2011, Mallik and Lakhotia, 2009, Mallik and Lakhotia, 2009, Mallik and Lakhotia, 2010) P{EP}Hsrω^EP93D (FlyBase, 1992-, Sengupta and Lakhotia, 2006, ) unnamed (Sengupta and Lakhotia, 2006)
Secondary FlyBase IDs

References ( 9 )
Research paper	Onorati et al., 2011, PLoS Genet. 7(5): e1002096 The ISWI Chromatin Remodeler Organizes the hsrω ncRNA–Containing Omega Speckle Nuclear Compartments. [FBrf0213856] Mallik and Lakhotia, 2010, Genetics 184(4): 927--945 Improved activities of CREB binding protein, heterogeneous nuclear ribonucleoproteins and proteasome following downregulation of noncoding hsromega transcripts help suppress poly(Q) pathogenesis in fly models. [FBrf0211742] Mallik and Lakhotia, 2009, RNA Biol. 6(4): 464--478 RNAi for the large non-coding hsromega transcripts suppresses polyglutamine pathogenesis in Drosophila models. [FBrf0209246] Mallik and Lakhotia, 2009, Genetics 183(3): 831--852 The developmentally active and stress-inducible noncoding hsromega gene is a novel regulator of apoptosis in Drosophila. [FBrf0209343] Sengupta and Lakhotia, 2006, RNA Biol. 3(1): e1--e8 Altered expressions of the noncoding hsromega gene enhances poly-Q-induced neurotoxicity in Drosophila. [FBrf0199067] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422 A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
FlyBase analysis	FlyBase Curators, 2013, Members of TE insertion collections. Members of TE insertion collections. [FBrf0220668] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{EP}HsrωEP93D Insertion

Dmel\P{EP}Hsrω^EP93D Insertion