FlyBase Insertion Report: Dmel\P{GawB}NP5021

General Information

Symbol

Dmel\P{GawB}NP5021

Species

D. melanogaster

Name

FlyBase ID

FBti0077139

Feature type

transposable_element_insertion_site

Also Known As

NP5021

Description

Inserted Element

P{GawB}

LINE ID

Affected gene(s)

Scer\GAL4

Viability / fertility

Associated allele(s)

Scer\GAL4^NP5021

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

Sequence Location

Target / Docking site

Member of Large Scale Dataset(s)

Dataset

TI_set_P{GawB}.NP

Description

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w^+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.

(Hayashi et al., 2002)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

Orientation

Cytological location (computed by FlyBase)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

Inserted Element

Construct

P{GawB}

(FlyBase, 1992-)

Size (Kb)

11.279

Component Allele(s)

Scer\GAL4^wB

w^+mW.hs

Molecular map

Affected Gene(s)

Insertion may affect gene

Scer\GAL4

(Nakayama et al., 2011, Okumura et al., 2010, Wang et al., 2009, Hozumi et al., 2006, Kamikouchi et al., 2006)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Scer\GAL4^NP5021

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 9 -- 17

embryonic/larval gut

(Hozumi et al., 2006)

larval stage

embryonic/larval salivary gland

(Wang et al., 2009)

embryonic/larval proventriculus

(Wang et al., 2009)

embryonic/larval middle midgut

(Wang et al., 2009)

embryonic/larval Malpighian tubule

(Wang et al., 2009)

embryonic/larval hindgut

(Wang et al., 2009)

humeral disc

(Wang et al., 2009)

labial disc

(Wang et al., 2009)

embryonic/larval anal pad

(Wang et al., 2009)

adult stage

zone A Johnston organ neuron | subset

(Kamikouchi et al., 2006)

adult antennal lobe projection neuron DL4 adPN

(Tanaka et al., 2012)

subregion AA of AMMC zone A

(Flybrain Neuron Database et al., 2018.6.28)

subregion AP of AMMC zone A

(Flybrain Neuron Database et al., 2018.6.28)

subregion AD of AMMC zone A

(Flybrain Neuron Database et al., 2018.6.28)

subregion AV2 of AMMC zone A

(Flybrain Neuron Database et al., 2018.6.28)

Additional Information

Statement

Reference

ScerGAL4^NP5021 drives expression in one uniglomerular antennal lobe projection neuron DL4 adPN. Its cell body is positioned in the anterior-dorsal part of the antennal lobe.

(Tanaka et al., 2012)

ScerGAL4^NP5021 drives expression in the entire gut from embryonic stage 9.

(Hozumi et al., 2006)

Marker for

Reflects expression of

Reporter construct used in assay

Mmus\Cd8a^UAS.cLa.EGFP

(Tanaka et al., 2012)

Moe^UAS.GFP

(Hozumi et al., 2006)

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Kyoto

113545

w^* P{GawB}NP5021 / FM7c

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

JO25

(Kamikouchi et al., 2006)

NP5021

(Lai et al., 2023, Flybrain Neuron Database et al., 2018.6.28, Nakayama et al., 2014, Kuroda et al., 2012, Tanaka et al., 2012, Nakayama et al., 2011, Okumura et al., 2010, Wang et al., 2009, Hozumi et al., 2006, Kamikouchi et al., 2006)

P{GawB}NP5021

(FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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