A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\Mi{ET1}TyrRIIMB03028 Insertion

General Information
Symbol Dmel\Mi{ET1}TyrRIIMB03028 Species D. melanogaster
Name FlyBase ID FBti0077604
Feature type transposable_element_insertion_site
Description
Inserted element Mi{ET1} Expression data
Affected gene(s) TyrRII Viability / fertility fertile, viable
Causes allele(s) TyrRIIMB03028 Stock availability 1 publicly available
LINE ID MB03028
Genomic Location
Chromosomal location 3R ( 90C2 ) Sequence location 3R:13,567,316..13,567,316 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBti0062811 FBti0058132 FBti0152253 FBti0077604
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the Dhyd\Minos-based construct Mi{ET1}; vetted by the Gene Disruption Project (GDP). The Mi{ET1} construct carries the Avic\GFP[E.3xP3] fluorescent marker, a Scer\GAL4 driver/reporter gene, and bacterial sequences that allow plasmid rescue.
Additional Mi{ET1} insertion lines were generated, mapped, and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
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Description
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FB2013_03
FB2013_02
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Chromosome (arm)
Sequence Location
3R:13,567,316..13,567,316 [-]
Orientation
Cytological location
(computed by FlyBase)
90C2 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
90C2 (reported as inferred from sequence location)
Comments concerning
location
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Flanking sequence
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Construct Mi{ET1}
Location-dependent
role
GAL4 driver/enhancer trap
Size 7.5, 7.5Kb
Associated alleles
Molecular map
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Insertion may
affect gene
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Causes alleles
Lethality
References
viable
Sterility
References
fertile
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Statement
Reference
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Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
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FlyView (LinkOut)
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Line ID
MB03028
Origin as a multiple insertion line
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Aberration
Balancer
hide Stocks ( 1 )
Bloomington
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Reported As
Symbol Synonym
Mi{ET1}TyrRIIMB03028
Secondary FlyBase IDs
hide References ( 5 )
Research paper
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Metaxakis et al., 2005, Genetics 171(2): 571--581
Minos as a genetic and genomic tool in Drosophila melanogaster. [FBrf0190750]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Personal communication to FlyBase
Bellen, 2007.5.7, batch741.xml
batch741.xml [FBrf0199562]
FlyBase analysis
FlyBase Curators, 2013, Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}.
Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}. [FBrf0220667]