A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{EPgy2}CG1021EY08071 Insertion

General Information
Symbol Dmel\P{EPgy2}CG1021EY08071 Species D. melanogaster
Name FlyBase ID FBti0100346
Feature type transposable_element_insertion_site
Description
Inserted element P{EPgy2} Expression data
Affected gene(s) CG1021 Viability / fertility fertile, viable
Causes allele(s) CG1021[EY08071] Stock availability 1 publicly available
LINE ID EY08071
Genomic Location
Chromosomal location 3R ( 83E6 ) Sequence location 3R:2,231,539..2,231,539 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0063873 FBti0053604 FBti0037636 FBti0043398 FBti0043468 FBti0108460 FBti0103648 FBti0045667 FBti0045668 FBti0037065 FBti0100346 FBti0104571 FBti0109731 FBti0070732 FBti0104170 FBti0110959 FBti0018075 FBti0072185 FBti0107647 FBti0099961 FBti0105916 FBti0105207 FBti0108228 FBti0109315 FBti0055045 FBti0125935_1 FBti0047293 FBti0053407 FBti0125935_2 FBti0040083 FBti0052786 FBti0044729 FBti0051423 FBti0132554 FBti0066581
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EPgy2}; created and vetted by the Gene Disruption Project (GDP). The P{EPgy2} construct carries two visible markers, the mini-white marker w[+mC] and the mini-yellow marker y[+mDint2], and Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.
Additional P{EPgy2} insertion lines were generated, mapped, and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
3R:2,231,539..2,231,539 [+]
Orientation
Cytological location
(computed by FlyBase)
83E6 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
83E6 (reported as inferred from sequence location)
Comments concerning
location
hide Sequence Data
Flanking sequence
hide Inserted Element
Construct P{EPgy2}
Location-dependent
role
mobile activating element (UASG)
Size 10.908Kb
Associated alleles
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
hide Alleles and Phenotypes
Causes alleles
Lethality
References
Sterility
References
fertile
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
EY08071
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
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hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{EPgy2}CG1021EY08071
Secondary FlyBase IDs
hide References ( 4 )
Research paper
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Personal communication to FlyBase
Bellen, 2006.4.12, Batch655.xml
Batch655.xml [FBrf0200401]
FlyBase analysis
FlyBase Curators, 2013, Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}.
Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}. [FBrf0220667]