Dmel\P{GawB}c305a Insertion

General Information
Symbol	Dmel\P{GawB}c305a	Species	D. melanogaster
Name		FlyBase ID	FBti0100741
Feature type	transposable_element_insertion_site
Description
Inserted element	P{GawB}	Expression data	GAL4 reporter/driver
Affected gene(s)	Scer\GAL4	Viability / fertility
Causes allele(s)	Scer\GAL4^c305a	Stock availability	1 publicly available
LINE ID
Genomic Location
Chromosomal location	2	Sequence location
Member of Large Scale Dataset(s)
Dataset
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)	2 (Cook, 2010.7.28)
Sequence Location
Orientation
Cytological location (computed by FlyBase)
Cytological location (reported)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{GawB}
Location-dependent role	GAL4 driver/enhancer trap (Brand and Perrimon, 1993)
Size	11.279Kb
Associated alleles	Scer\GAL4^wB w^+mW.hs
Molecular map
Affected Gene(s)
Insertion may affect gene	Scer\GAL4 (Lin et al., 2007, Chen et al., 2012, van Swinderen et al., 2009, Blum et al., 2009, Keleman et al., 2007, Krashes et al., 2007, Hekmat-Scafe et al., 2010, Tan et al., 2010, Buchanan and Davis, 2010, Mao and Davis, 2009, Christiansen et al., 2011, Lee et al., 2011, Aso et al., 2009, Pauls et al., 2010, Pauls et al., 2010, Ueno et al., 2013, Keleman et al., 2012)
Alleles and Phenotypes
Causes alleles	Scer\GAL4^c305a (Lin et al., 2007, Chen et al., 2012, van Swinderen et al., 2009, Blum et al., 2009, Keleman et al., 2007, Krashes et al., 2007, Hekmat-Scafe et al., 2010, Tan et al., 2010, Buchanan and Davis, 2010, Mao and Davis, 2009, Christiansen et al., 2011, Lee et al., 2011, Aso et al., 2009, Pauls et al., 2010, Pauls et al., 2010, Ueno et al., 2013, Keleman et al., 2012)

Lethality References
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS) Stage Tissue/Position (including subcellular localization) Reference adult stage adult antennal lobe (Krashes et al., 2007, Aso et al., 2009) ellipsoid body \| restricted (Krashes et al., 2007) adult antennal nerve \| restricted (Krashes et al., 2007) adult pedunculus \| distal & restricted (Mao and Davis, 2009) alpha'/beta' Kenyon cell (Buchanan and Davis, 2010, Christiansen et al., 2011) neuron \| subset of adult brain (Chen et al., 2012) adult mushroom body (Chen et al., 2012) Comment:medium expression glial cell of adult brain (Chen et al., 2012) Comment:faint expression ellipsoid body (Chen et al., 2012, Aso et al., 2009) Comment:medium expression eye (Chen et al., 2012) Comment:faint expression adult mushroom body beta'-lobe (Krashes et al., 2007, Mao and Davis, 2009, Tan et al., 2010) adult mushroom body alpha'-lobe (Krashes et al., 2007, Tan et al., 2010) mushroom body alpha' anterior stratum \| intense (Aso et al., 2009) mushroom body beta' anterior stratum \| intense (Aso et al., 2009) mushroom body alpha' middle stratum (Aso et al., 2009) mushroom body beta' middle stratum (Aso et al., 2009) mushroom body alpha' posterior stratum \| intense (Aso et al., 2009) mushroom body beta' posterior stratum \| intense (Aso et al., 2009) medulla \| faint (Aso et al., 2009) adult protocerebrum \| restricted (Aso et al., 2009) inferior neuropils (Aso et al., 2009) pars intercerebralis \| faint (Aso et al., 2009) adult antennal nerve \| intense (Aso et al., 2009) adult tritocerebrum (Aso et al., 2009) adult subesophageal ganglion (Aso et al., 2009)
Additional Information
Statement Reference Scer\GAL4[c305a] labels the mushroom body alpha'/beta' neurons. Co-expression with a postsynaptic marker labels Kenyon cell terminals in the mushroom body alpha' and beta' lobes. No presynaptic terminals in the calyx are observed. (Christiansen et al., 2011, Christiansen et al., 2011) Expression of Scer\GAL4[c305a] in the adult antennal nerve is in putative mechanosensory neurons, and within the ring neurons of the ellipsoid body. (Krashes et al., 2007)

Marker for	mushroom body alpha'/beta' neuron (Christiansen et al., 2011) mushroom body alpha'-lobe (Krashes et al., 2007) mushroom body beta'-lobe (Krashes et al., 2007)
Reflects expression of
Reporter construct used in assay	brp^{S.Scer\UAS.T:Disc\RFP-mCherry} (Christiansen et al., 2011) gish^L.Scer\UAS (Tan et al., 2010) gish^S.Scer\UAS (Tan et al., 2010) Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} (Krashes et al., 2007, Mao and Davis, 2009, Aso et al., 2009) Nf1^Scer\UAS.cBa (Buchanan and Davis, 2010) P{UAS-gfA.GFP} (Christiansen et al., 2011) Scer\GAL80^Mef2.PT (Krashes et al., 2007) shi^1.Scer\UAS (Krashes et al., 2007)
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	30829 w^*; P{GawB}c305a
Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	C305 (Pauls et al., 2010) c305a (Chen et al., 2012, Ueno et al., 2013, van Swinderen et al., 2009, Krashes et al., 2007, Hekmat-Scafe et al., 2010, Tan et al., 2010, Pauls et al., 2010, Lee et al., 2011, Pauls et al., 2010, Aso et al., 2009, Keleman et al., 2012) C305a (Blum et al., 2009) c305a{GAL4} (Krashes et al., 2007) c305a-GAL4 (Keleman et al., 2007, Mao and Davis, 2009, Christiansen et al., 2011) c305a-gal4 (Buchanan and Davis, 2010) GAL4-c305a (Lin et al., 2007) P{GAL4}c305a (FlyBase, 1992-) P{GawB}c305a (Cook, 2010.7.28, )
Secondary FlyBase IDs

References ( 20 )
Research paper	Ueno et al., 2013, J. Physiol. 591(1): 287--302 Long-term enhancement of synaptic transmission between antennal lobe and mushroom body in cultured Drosophila brain. [FBrf0220358] Chen et al., 2012, Science 335(6069): 678--685 Visualizing long-term memory formation in two neurons of the Drosophila brain. [FBrf0217453] Keleman et al., 2012, Nature 489(7414): 145--149 Dopamine neurons modulate pheromone responses in Drosophila courtship learning. [FBrf0219398] Christiansen et al., 2011, J. Neurosci. 31(26): 9696--9707 Presynapses in Kenyon Cell Dendrites in the Mushroom Body Calyx of Drosophila. [FBrf0214059] Lee et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(33): 13794--13799 Serotonin-mushroom body circuit modulating the formation of anesthesia-resistant memory in Drosophila. [FBrf0214737] Buchanan and Davis, 2010, J. Neurosci. 30(30): 10135--10143 A distinct set of Drosophila brain neurons required for neurofibromatosis type 1-dependent learning and memory. [FBrf0211411] Hekmat-Scafe et al., 2010, Genetics 184(1): 171--183 Seizure sensitivity is ameliorated by targeted expression of k+-cl- cotransporter function in the mushroom body of the Drosophila brain. [FBrf0209683] Pauls et al., 2010, J. Neurosci. 30(32): 10655--10666 Drosophila Larvae Establish Appetitive Olfactory Memories via Mushroom Body Neurons of Embryonic Origin. [FBrf0211533] Tan et al., 2010, Neuron 67(5): 810--820 Gilgamesh Is Required for rutabaga-Independent Olfactory Learning in Drosophila. [FBrf0211760] Aso et al., 2009, J. Neurogenet. 23(1): 156--172 The mushroom body of adult Drosophila characterized by GAL4 drivers. [FBrf0207600] Blum et al., 2009, Curr. Biol. 19(16): 1341--1350 Short- and long-term memory in Drosophila require cAMP signaling in distinct neuron types. [FBrf0208576] Mao and Davis, 2009, Front. Neural Circuits 3: 5 Eight different types of dopaminergic neurons innervate the Drosophila mushroom body neuropil: anatomical and physiological heterogeneity. [FBrf0208427] van Swinderen et al., 2009, PLoS ONE 4(6): e5989 Shared visual attention and memory systems in the Drosophila brain. [FBrf0208235] Keleman et al., 2007, Nat. Neurosci. 10(12): 1587--1593 Function of the Drosophila CPEB protein Orb2 in long-term courtship memory. [FBrf0202837] Krashes et al., 2007, Neuron 53(1): 103--115 Sequential use of mushroom body neuron subsets during Drosophila odor memory processing. [FBrf0193427] Lin et al., 2007, Cell 128(6): 1205--1217 A map of olfactory representation in the Drosophila mushroom body. [FBrf0201372] Brand and Perrimon, 1993, Development 118(2): 401--415 Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. [FBrf0064375]
Supplementary material	Pauls et al., 2010, J. Neurosci. 30(32): Supplemental Material. [FBrf0220210]
Personal communication to FlyBase	Cook, 2010.7.28, Mapping P{GawB} insertions. Mapping P{GawB} insertions. [FBrf0211367]
FlyBase analysis	FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]