A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{XP}d09482 Insertion

General Information
Symbol Dmel\P{XP}d09482 Species D. melanogaster
Name FlyBase ID FBti0101523
Feature type transposable_element_insertion_site
Description
Inserted element P{XP} Expression data
Affected gene(s) Viability / fertility
Causes allele(s) Stock availability none publicly available
LINE ID d09482
Genomic Location
Chromosomal location X ( 10F7 ) Sequence location X:11,815,319..11,815,319
Map ( GBrowse ) GBrowse View Help detailed view FBti0041835 FBti0040227 FBti0051552 FBti0148425 FBti0101522 FBti0101523 FBti0067682 FBti0068313 FBti0147205 FBti0034070 FBti0017645 FBti0069760 FBti0056797 FBti0049813 FBti0033898 FBti0050927 FBti0139995
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{XP}. The P{XP} construct carries the w[+mC] mini-white marker, long (199-bp) Scer\FRT sites, and is designed to allow flexible use of Scer\UAS sites for Scer\GAL4-driven misexpression of adjacent genes. FRT sites allow Scer\FLP-mediated recombination between other FRT-containing elements, and thus can be used to generate molecularly defined deletions.
P{XP} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
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Description
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FB2013_03
FB2013_02
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Chromosome (arm)
Sequence Location
X:11,815,319..11,815,319
Orientation
Cytological location
(computed by FlyBase)
10F7 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
Comments concerning
location
Exelixis, Inc. determined the insertion site of P{XP}d09482 to be Release 3 genomic coordinate 11651944 on the X chromosome. This corresponds to 10F7 on the Release 3 and Release 5 genome maps.
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Flanking sequence
hide Inserted Element
Construct P{XP}
Location-dependent
role
deletion generation component
mobile activating element
carries FRT site(s)
Size 7.303Kb
Associated alleles
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
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Causes alleles
Lethality
References
Sterility
References
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Statement
Reference
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Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
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FlyView (LinkOut)
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Line ID
d09482
Origin as a multiple insertion line
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hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 0 )
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Reported As
Symbol Synonym
Secondary FlyBase IDs
hide References ( 7 )
Research paper
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Thibault et al., 2004, Nat. Genet. 36(3): 283--287
A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Personal communication to FlyBase
Cook et al., 2010.5.7, Isolation and characterization of Df(1)BSC876.
Isolation and characterization of Df(1)BSC876. [FBrf0210856]
Christensen et al., 2008.6.11, Isolation and characterization of Df(1)BSC543.
Isolation and characterization of Df(1)BSC543. [FBrf0204984]
Christensen et al., 2008.6.11, Isolation and characterization of Df(1)BSC542.
Isolation and characterization of Df(1)BSC542. [FBrf0204983]
FlyBase analysis
FlyBase Curators, 2013, Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}.
Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. [FBrf0221061]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]