A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{GSV6}pucGS16811 Insertion

General Information
Symbol Dmel\P{GSV6}pucGS16811 Species D. melanogaster
Name FlyBase ID FBti0108946
Feature type transposable_element_insertion_site
Description
Inserted element P{GSV6} Expression data
Affected gene(s) puc Viability / fertility
Causes allele(s) pucGS16811 Stock availability 1 publicly available
LINE ID
Genomic Location
Chromosomal location 3R ( 84E13 ) Sequence location 3R:3,945,585..3,945,585
Map ( GBrowse ) GBrowse View Help detailed view FBti0045283 FBti0051008 FBti0050878 FBti0021404 FBti0108197 FBti0065613 FBti0047018 FBti0052119 FBti0108946 FBti0106735 FBti0057251 FBti0034375 FBti0056755 FBti0106557 FBti0044520 FBti0045957 FBti0067286 FBti0033496 FBti0039848 FBti0017163 FBti0109921 FBti0013471 FBti0103611 FBti0029748 FBti0102920 FBti0105427 FBti0041390
Member of Large Scale Dataset(s)
Dataset
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
3R:3,945,585..3,945,585
(FlyBase, 2009.6.5)
Orientation
Cytological location
(computed by FlyBase)
84E13 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
84E13 (reported as inferred from sequence location)
(Muramatsu et al., 2007.6.13)
Comments concerning
location
hide Sequence Data
Flanking sequence
AB320466
(Muramatsu et al., 2007.6.13)
AB320467
(Muramatsu et al., 2007.6.13)
hide Inserted Element
Construct P{GSV6}
Location-dependent
role
mobile activating element (UASG)
(Aigaki, 2003.4.9)
Size 6.836, 6.836Kb
Associated alleles
Avic\GFPScer\UAS.cAa
w+mGS
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
puc
(Okumura et al., 2010, Taniguchi et al., 2007)
hide Alleles and Phenotypes
Causes alleles
pucGS16811
(Okumura et al., 2010, Taniguchi et al., 2007)
Lethality
References
Sterility
References
hide Phenotype Manifest In
anterior embryonic/larval midgut
(Taniguchi et al., 2007)
embryonic/larval proventriculus
(Taniguchi et al., 2007)
hide Detailed Description
Statement
Reference
Expression of puc[Scer\UAS.cMa] under the control of either Scer\GAL4[48Y] or Scer\GAL4[Gap1-NP3392] completely rescues the left-right asymmetry defects seen in the anterior midgut of puc[GS16811] embryos. The defects in dorsal closure are not rescued. The lack of left-right asymmetry in the tilting of the nuclei of the circular visceral muscle which is seen in late stage 15 and 16 puc[GS16811] embryos is rescued by expression of puc[Scer\UAS.cMa] under the control of Scer\GAL4[Gap1-NP3392]. Expression of puc[Scer\UAS.cMa] under the control of either Scer\GAL4[dpp.PS] or Scer\GAL4[NP7020] does not rescue the left-right asymmetry defects seen in the anterior midgut of puc[GS16811] embryos. Expression of puc[Scer\UAS.cMa] under the control of Scer\GAL4[hs.PU] using heat shock for 30 minutes at 37[o]C significantly rescues the left-right asymmetry defects seen in the anterior midgut of puc[GS16811] embryos when the heat shock is applied at any time during 4.5 to 9.5 AEL.
(Taniguchi et al., 2007)
At stage 17, 37% of homozygous embryos show inversion of the normal left-right asymmetry of the anterior midgut, 14% do not show any left-right asymmetry and 49% show normal left-right asymmetry. In addition the valvular structure of the proventriculus fails to form and the embryos fail to complete dorsal closure. No difference is detected between the tilting of the nuclei of the circular visceral muscle (CVM) cells on the right and left sides of the presumptive first midgut constriction in late stage 15 and 16 homozygous embryos. This contrasts with wild-type embryos at these stages, where the nuclei of a subset of CVM cells in the right ventral region of the presumptive first chamber of the midgut become tilted compared with those in the left-ventral region. 38% of stage 17 puc[GS16811]/Df(3R)dsx10D embryos show inversion of the normal left-right asymmetry of the anterior midgut and 7% do not show any left-right asymmetry.
(Taniguchi et al., 2007)
bsk[1]/+ significantly suppresses the defects in left-right asymmetry of the anterior midgut that are seen in homozygous puc[GS16811] embryos. Expression of bsk[DN.Scer\UAS] under the control of Scer\GAL4[48Y] significantly suppresses the defects in left-right asymmetry of the anterior midgut that are seen in homozygous puc[GS16811] embryos. Expression of bsk[DN.Scer\UAS] under the control of Scer\GAL4[Gap1-NP3392] significantly suppresses the defects in left-right asymmetry of the anterior midgut that are seen in homozygous puc[GS16811] embryos. This suppression is stronger if the embryos are also carrying bsk[1]/+. puc[GS16811]/+ significantly enhances the defects in left-right asymmetry of the anterior midgut which are seen in embryos expressing Rac1[V12.Scer\UAS] under the control of Scer\GAL4[Gap1-NP3392]. 20% of the double mutant embryos show inversion of the normal left-right asymmetry and 42% show no left-right asymmetry. puc[GS16811]/+ has no effect on the defects in left-right asymmetry of the anterior midgut which are seen in embryos expressing Rho1[V14.Scer\UAS] under the control of Scer\GAL4[Gap1-NP3392]. puc[GS16811]/+ has no effect on the defects in left-right asymmetry of the anterior midgut which are seen in embryos expressing Cdc42[V12.Scer\UAS] under the control of Scer\GAL4[Gap1-NP3392].
(Taniguchi et al., 2007)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
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FlyView (LinkOut)
hide Data on Genetic Line
Line ID
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Kyoto
206780
y1 w67c23; P{GSV6}pucGS16811/TM3, Sb1 Ser1
hide Linkouts
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
16811
(Muramatsu et al., 2007.6.13)
P{GSV6}GS16811
(FlyBase, 1992-)
P{GSV6}pucGS16811
 
pucGS16811
(Okumura et al., 2010)
Secondary FlyBase IDs
hide References ( 7 )
Research paper
Okumura et al., 2010, Dev. Biol. 344(2): 693--706
Left-right asymmetric morphogenesis of the anterior midgut depends on the activation of a non-muscle myosin II in Drosophila. [FBrf0211383]
Taniguchi et al., 2007, Dev. Biol. 311(1): 251--263
D-JNK signaling in visceral muscle cells controls the laterality of the Drosophila gut. [FBrf0200469]
Personal communication to FlyBase
Muramatsu et al., 2007.6.13, GS vector insertion sites, flanking regions.
GS vector insertion sites, flanking regions. [FBrf0200395]
Aigaki, 2003.4.9, The GS6 vector.
The GS6 vector. [FBrf0159686]
FlyBase analysis
FlyBase, 2009.6.5, Mapping of GS vector insertion sites to genome release 5.
Mapping of GS vector insertion sites to genome release 5. [FBrf0206797]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]
Website
Drosophila Gene Search Project, 2012, GS vector structures and sequences
GS vector structures and sequences [FBrf0220214]