Dmel\P{EP}jumu^GE27806 Insertion

General Information
Symbol	Dmel\P{EP}jumu^GE27806	Species	D. melanogaster
Name		FlyBase ID	FBti0115177
Feature type	transposable_element_insertion_site
Description
Inserted element	P{EP}	Expression data
Affected gene(s)	jumu	Viability / fertility
Causes allele(s)	jumu^GE27806	Stock availability	1 publicly available
LINE ID
Genomic Location
Chromosomal location	3R ( 86B1 )	Sequence location	3R:6,175,969..6,175,969 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{EP}.G A set of transgenic insertion stocks derived by TE mobilization using the using the P-element construct P{EP}. The P{EP} construct construct carries a w[+mC] mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue (FBrf0090768). The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity. (Bellen et al., 2011)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	3R:6,175,969..6,175,969 [+] (Levis, 2009.3.18)
Orientation	+ (Levis, 2009.3.18)
Cytological location (computed by FlyBase)	86B1 ( inferred by FlyBase from sequence location )
Cytological location (reported)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{EP}
Location-dependent role	mobile activating element (UASG) (Rorth, 1996)
Size	7.987Kb
Associated alleles	Scer\UAS^14x.hs TrlBR^cRa w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	jumu (Levis, 2009.3.18, Jin et al., 2008)
Alleles and Phenotypes
Causes alleles	jumu^GE27806 (Levis, 2009.3.18, Jin et al., 2008)

Lethality References
Sterility References
Phenotype Manifest In
	hemocyte \| adult stage (Jin et al., 2008) plasmatocyte \| third instar larval stage (Jin et al., 2008)
Detailed Description
Statement Reference Over-expressing jumu using the UAS sequences in jumu[GE27806] under the control of Scer\GAL4[hs.PU] rescues the sensitivity to fungal infection seen in this line. (Jin et al., 2008) Homozygous jumu[GE27806] mutant adults are more sensitive to fungal infection (B. bassiana) than wild type controls. This increase in sensitivity is seen following both natural infection and infection through septic injury. No reduction in survival is seen following wounding or in response to infection with Gram-negative bacteria (Ecc-15). A fall in survival rate is seen in response to the Gram-positive bacteria S. aureus, but not M. luteus. An increased number of hemocytes are seen in jumu[GE27806] mutants compared to wild type but overall hemocyte uptake of B. bassiana fungal spores is similar to wild type. Defects are also seen in the phagocytic uptake of S. aureus but not E. coli. An increased number of plasmatocytes is seen in homozygous jumu[GE27806] third instar larvae. The number of crystal cells is similar to wild type and, as in wild type, strong melanisation occurs in response to injury. (Jin et al., 2008)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	26897 w^*; P{EP}jumu^GE27806
Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	G5217 (Levis, 2009.3.18) P{EP}jumu^GE27806 (FlyBase, 1992-, Levis, 2009.3.18, ) unnamed (Jin et al., 2008)
Secondary FlyBase IDs

References ( 6 )
Research paper	Bellen et al., 2011, Genetics 188(3): 731--743 The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229] Jin et al., 2008, PLoS Pathog. 4(10): e1000168 Identification and functional analysis of antifungal immune response genes in Drosophila. [FBrf0206045] Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422 A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
Personal communication to FlyBase	Levis, 2009.3.18, Genomic coordinates of P{EP} insertions in antifungal genes. Genomic coordinates of P{EP} insertions in antifungal genes. [FBrf0207426]
FlyBase analysis	FlyBase Curators, 2013, Members of TE insertion collections. Members of TE insertion collections. [FBrf0220668] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{EP}jumuGE27806 Insertion

Dmel\P{EP}jumu^GE27806 Insertion