A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{EP}RecQ4G7470 Insertion

General Information
Symbol Dmel\P{EP}RecQ4G7470 Species D. melanogaster
Name FlyBase ID FBti0115604
Feature type transposable_element_insertion_site
Description
Inserted element P{EP} Expression data
Affected gene(s) RecQ4 Viability / fertility
Causes allele(s) RecQ4G7470 Stock availability 1 publicly available
LINE ID G7470
Genomic Location
Chromosomal location 3L ( 66B10 ) Sequence location 3L:8,168,992..8,168,992 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0143236 FBti0115604 FBti0004908 FBti0065089 FBti0149163 FBti0114884 FBti0113080 FBti0024835 FBti0102722 FBti0036613 FBti0102965
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the using the P-element construct P{EP}. The P{EP} construct construct carries a w[+mC] mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue (FBrf0090768). The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.
(Bellen et al., 2011)
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
3L:8,168,992..8,168,992 [+]
(Bellen, 2009.4.8)
Orientation
Cytological location
(computed by FlyBase)
66B10 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
66B10 (reported as inferred from sequence location)
(Bellen, 2009.4.8)
Comments concerning
location
hide Sequence Data
Flanking sequence
FI847665
(Bellen, 2009.4.8)
hide Inserted Element
Construct P{EP}
Location-dependent
role
mobile activating element (UASG)
(Rorth, 1996)
Size 7.987Kb
Associated alleles
Scer\UAS14x.hs
TrlBRcRa
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
RecQ4
(Bellen, 2009.4.8, Wu et al., 2008)
hide Alleles and Phenotypes
Causes alleles
RecQ4G7470
(Bellen, 2009.4.8, Wu et al., 2008)
Lethality
References
partially lethal - majority live
(Wu et al., 2008)
Sterility
References
female sterile
(Wu et al., 2008)
hide Phenotype Manifest In
egg
(Wu et al., 2008)
spiracle
(Wu et al., 2008)
hide Detailed Description
Statement
Reference
RecQ4[T:Avic\GFP] fully rescues the infertility of RecQ4[G7470] females.
(Wu et al., 2008)
RecQ4[G7470] flies are homozygous viable, albeit with a low viability. While male fertlity is unaffected, females are sterile, due to a defect in oogenesis. RecQ4[G7470] mutant females lay eggs with fragile eggshells and hatch embryos with spiracle defects. No apparent defect is observed in follicle cells during mitotic cycles and endocycles before stage 10B. BrdU incorporation in chorion gene clusters on both the X and third chromosome is greatly reduced in follicle cells of hypomorphic mutants, with incorporation decreased about 5-fold compared to controls.
(Wu et al., 2008)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
G7470
(Bellen, 2009.4.8)
Origin as a multiple insertion line
N
(Bellen, 2009.4.8)
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
27228
y1 w*; P{EP}RecQ4G7470/TM3, Sb1 Ser1
hide Linkouts
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
G7470
(Wu et al., 2008)
P{EP}RecQ4G7470
(Bellen, 2009.4.8, )
recq4EP
(Wu et al., 2008)
Secondary FlyBase IDs
hide References ( 6 )
Research paper
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Wu et al., 2008, Dev. Biol. 323(1): 130--142
Drosophila homologue of the Rothmund-Thomson syndrome gene: essential function in DNA replication during development. [FBrf0209176]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422
A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
Personal communication to FlyBase
Bellen, 2009.4.8, BDGP_2009_0408.GenExel.xml
BDGP_2009_0408.GenExel.xml [FBrf0207636]
FlyBase analysis
FlyBase Curators, 2013, Members of TE insertion collections.
Members of TE insertion collections. [FBrf0220668]