Dmel\P{EP}bchs^EP2299 Insertion

General Information
Symbol	Dmel\P{EP}bchs^EP2299	Species	D. melanogaster
Name		FlyBase ID	FBti0115740
Feature type	transposable_element_insertion_site
Description
Inserted element	P{EP}	Expression data
Affected gene(s)	bchs	Viability / fertility
Causes allele(s)	bchs¹², bchs¹⁷, bchs⁵⁸, bchs^EP2299	Stock availability	2 publicly available
LINE ID	EP(2)2299
Genomic Location
Chromosomal location	2L ( 26A1 )	Sequence location	2L:5,907,523..5,907,523 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{EP}.EP A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EP}. The P{EP} construct construct carries a w[+mC] mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity. Insertion lines from this collection were mapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank. (Rorth, 1996, Bellen et al., 2004)
Recent Updates
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	2L:5,907,523..5,907,523 [+] (BDGP Project Members, 2000-)
Orientation
Cytological location (computed by FlyBase)	26A1 ( inferred by FlyBase from sequence location )
Cytological location (reported)	26A1-26A2 (in situ hybridization reported) (BDGP Project Members, 1994-1999) 26A6-26A6 (reported as inferred from sequence location) (BDGP Project Members, 2000-)
Comments concerning location
Sequence Data
Flanking sequence	AQ254846 (FlyBase, 1992-)
Inserted Element
Construct	P{EP}
Location-dependent role	mobile activating element (UASG) (Rorth, 1996)
Size	7.987Kb
Associated alleles	Scer\UAS^14x.hs TrlBR^cRa w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	bchs (Eissenberg et al., 2011, BDGP Project Members, 2000-, Simonsen et al., 2007, Khodosh et al., 2006, Kriston-Vizi et al., 2011, Raymond et al., 2004, Luan et al., 2012)
Alleles and Phenotypes
Causes alleles	bchs¹² (Khodosh et al., 2006) bchs¹⁷ (Khodosh et al., 2006) bchs⁵⁸ (Khodosh et al., 2006) bchs^EP2299 (Eissenberg et al., 2011, Abdelilah-Seyfried et al., 2000, Finley et al., 2003, Gene Disruption Project members, 2001-, Kraut et al., 2001, Kraut et al., 2001, Khodosh et al., 2006, Simonsen et al., 2007, Kriston-Vizi et al., 2011, Raymond et al., 2004, Luan et al., 2012)

Lethality References viable (Khodosh et al., 2006)
Sterility References female fertile (Finley et al., 2003) fertile (Khodosh et al., 2006) male fertile (Finley et al., 2003)
Phenotype Manifest In
	adult abdomen & microchaeta (Abdelilah-Seyfried et al., 2000) adult central nervous system (Finley et al., 2003) anterior fascicle & synapse (Kraut et al., 2001, Kraut et al., 2001) dorsocentral bristle (Abdelilah-Seyfried et al., 2000) embryonic/larval brain \| third instar larval stage (Kriston-Vizi et al., 2011) eye (Mueller et al., 2005, Khodosh et al., 2006, Simonsen et al., 2007, Eissenberg et al., 2011, Raymond et al., 2004) growth cone & eye photoreceptor cell (Simonsen et al., 2007) intersegmental nerve (Kraut et al., 2001) neuron & eye photoreceptor cell (Simonsen et al., 2007) ommatidium (Khodosh et al., 2006) scutellar bristle (Abdelilah-Seyfried et al., 2000)
Detailed Description
Statement Reference Overexpression of bchs[EP2299] in the eye under the control of Scer\GAL4[GMR.PF] results in a rough eye phenotype and age related neuronal degeneration. (Eissenberg et al., 2011) The bchs[EP2299]-overexpression phenotype in the eye is enhanced when Gga[dsRNA.Scer\UAS.1] is co-expressed under the control of Scer\GAL4[GMR.PF] resulting in a reduction in eye size and a marked bleaching in the middle of the eye. (Eissenberg et al., 2011) Expression of bchs[EP2299] in the developing nervous system, driven by Scer\GAL4[elav-C155] results in a 14.6% increase in brain volume when compared to controls. (Kriston-Vizi et al., 2011) bchs[58]/Df(2L)cl7 and bchs[58] homozygous mutants display a 15 and 17% reduction in the brain volumes of third instar larvae, respectively. (Kriston-Vizi et al., 2011) Flies expressing bchs^EP2299 under the control of Scer\GAL4^GMR.PF have a roughened eye surface and a slight decrease in overall eye size compared to controls. Animals expressing bchs^EP2299 under the control of Scer\GAL4^GMR.PF show alterations in the neural projection patterns of photoreceptor cells compared to wild-type in mid-pupae; the number and basic array of R7 and R8 axonal projections remain relatively normal, but there is a loss of growth cone morphology, premature termination of developing synapses and axonal swellings or varicosities are seen. (Simonsen et al., 2007) rb¹ is a complex modifier of the eye phenotype caused by expression of bchs^EP2299 under the control of Scer\GAL4^GMR.PF. g¹ is a complex modifier of the eye phenotype caused by expression of bchs^EP2299 under the control of Scer\GAL4^GMR.PF. cm¹ is a complex modifier of the eye phenotype caused by expression of bchs^EP2299 under the control of Scer\GAL4^GMR.PF. (Simonsen et al., 2007) Expression of bchs^EP2299 in third instar eye discs, under the control of Scer\GAL4^GMR.PF, does not affect the patterning of the disc. Expression in the developing retina results in photoreceptor growth cones with an unusually bulbous central region that are less expanded than wild-type growth cones. However, Scer\GAL4^GMR.PF>bchs^EP2299 growth cones appear to grow normally towards their targets. At the adult stage, flies of this genotype have small, glazed eyes that lack distinct ommatidia compared to those of wild-type flies. (Khodosh et al., 2006) Flies that are mutant for Df(3R)e-R1 show a strong enhancement of the Scer\GAL4^GMR.PF>bchs^EP2299 overexpression phenotype. Rab11 heterozygotes act as dominant enhancers of the Scer\GAL4^GMR.PF>bchs^EP2299 eye phenotype, resulting in adults with severely reduced eyes. The following alleles cause this enhancement: Rab11^j2D1, Rab11^93Bi, Rab11^ex1, Rab11^ex2, Rab11^ETo11, and Rab11^ETo3. (Khodosh et al., 2006) bchs¹² mutants show no defects in the larval visual system or embryonic central nervous system. (Khodosh et al., 2006) The viability of Rab11^ex1/Rab11^93Bi is increased when flies are also mutant for bchs¹⁷/bchs¹². The microchaeta loss of Rab11^ex1/Rab11^93Bi flies is completely suppressed by bchs¹⁷/bchs¹². Further, posterior scutellar bristle length of Rab11^ex1/Rab11^93Bi flies is restored significantly by bchs¹⁷/bchs¹². The bouton density and branching defects of Rab11^ex1/Rab11^93Bi larval NMJs at muscle 6/7 are significantly suppressed in bchs¹⁷/bchs¹²; Rab11^ex1/Rab11^93Bi double mutants. (Khodosh et al., 2006) Expression of bchs⁵⁸ under the control of Scer\GAL4^GMR.PF does not cause the small eye or growth cone phenotypes seen when wild-type bchs is overexpressed. bchs⁵⁸ mutants show no defects in the larval visual system or embryonic central nervous system. (Khodosh et al., 2006) The microchaeta loss of Rab11^93Bi flies is significantly suppressed by bchs⁵⁸/+ and is completely suppressed by bchs⁵⁸/bchs⁵⁸. (Khodosh et al., 2006) bchs¹⁷ mutants show no defects in the larval visual system or embryonic central nervous system. (Khodosh et al., 2006) The viability of Rab11^ex1/Rab11^93Bi is increased when flies are also mutant for either Df(2L)cl7/bchs¹⁷, bchs¹⁷/bchs¹² or bchs¹⁷/+. The microchaeta loss of Rab11^ex1/Rab11^93Bi flies is significantly suppressed by bchs¹⁷/+ and is completely suppressed by Df(2L)cl7/bchs¹⁷ or bchs¹⁷/bchs¹². Further, posterior scutellar bristle length of Rab11^ex1/Rab11^93Bi flies is restored partially by bchs¹⁷/+ and more fully by Df(2L)cl7/bchs¹⁷ or bchs¹⁷/bchs¹². The bouton density and branching defects of Rab11^ex1/Rab11^93Bi larval NMJs at muscle 6/7 are significantly suppressed in bchs¹⁷/bchs¹²; Rab11^ex1/Rab11^93Bi double mutants. (Khodosh et al., 2006) Expression of bchs^EP2299 under the control of Scer\GAL4^GMR.PF results in smaller eyes than normal which have a glossy appearance. (Mueller et al., 2005) Expression of bchs[EP2299] under the control of Scer\GAL4[GMR.PU] results in a rough eye phenotype. (Raymond et al., 2004) bchs^EP2299 homozygotes and bchs^EP2299/Df(2L)cl7 flies have a reduced average lifespan compared to wild-type. These flies also have age-dependent ubiquitinated aggregates, that appear to be insoluble, throughout the CNS. (Finley et al., 2003) Mutant larvae expressing bchs^EP2299 under the control of Scer\GAL4^elav-C155 have altered ISN synapses with abnormal bulges at the junctions between the axons and the synaptic branches. (Kraut et al., 2001) Mutant larvae expressing bchs^EP2299 under the control of Scer\GAL4^elav-C155 have reduced or abnormal synapses. (Kraut et al., 2001) Expression of bchs^EP2299 under the control of Scer\GAL4^sca-537.4 results in severe loss of microchaetae on the abdomen and loss of scutellar and dorsocentral macrochaetae. Sensory organs with one socket, no shaft and one sheath cell are seen. (Abdelilah-Seyfried et al., 2000)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	EP(2)2299 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	9887 y¹ w^; P{EP}bchs^EP2299, bchs⁵⁸/CyO 9888 y¹ w^; P{EP}bchs^EP2299, bchs¹⁷/CyO
Linkouts
Comments
	Location 2L:5907523-5907524 confirmed by FlyBase alignment of dbGSS accession AQ254846 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005) insertion of mobile activating element (BDGP Project Members, 1994-1999)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	bchs^EP2299 (Kriston-Vizi et al., 2011) EP(2)2299 (Khodosh et al., 2006, Raymond et al., 2004) EP(2L)2299 (Luan et al., 2012, Simonsen et al., 2007, Eissenberg et al., 2011) EP-bchs (Khodosh et al., 2006) P{EP}bchs^EP2299 (FlyBase, 2005, FlyBase, 1992-) P{EP}Beach1^EP2299 (Horn et al., 2003, FlyBase, 1992-) P{EP}EP2299 (FlyBase, 1992-) P{EP}EP2299^EP2299 (FlyBase, 1992-)
Secondary FlyBase IDs
	FBti0011017 FBti0076726 FBti0076727 FBti0076728
References ( 20 )
Research paper	Luan et al., 2012, PLoS ONE 7(9): e45163 The Unique GGA Clathrin Adaptor of Drosophila melanogaster Is Not Essential. [FBrf0219559] Eissenberg et al., 2011, Traffic 12(12): 1821--1838 Drosophila GGA Model: An Ultimate Gateway to GGA Analysis. [FBrf0216574] Kriston-Vizi et al., 2011, BMC Bioinformatics 12: 232 Gebiss: an ImageJ plugin for the specification of ground truth and the performance evaluation of 3d segmentation algorithms. [FBrf0214312] Simonsen et al., 2007, Genetics 176(2): 1283--1297 Genetic modifiers of the Drosophila blue cheese gene link defects in lysosomal transport with decreased life span and altered ubiquitinated-protein profiles. [FBrf0201586] Khodosh et al., 2006, Development 133(23): 4655--4665 Bchs, a BEACH domain protein, antagonizes Rab11 in synapse morphogenesis and other developmental events. [FBrf0195140] Mueller et al., 2005, Genetics 171(3): 1137--1152 Genetic modifier screens on hairless gain-of function phenotypes reveal genes involved in cell differentiation, cell growth and apoptosis in Drosophila melanogaster. [FBrf0190751] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Raymond et al., 2004, J. Cell Sci. 117(13): 2777--2789 A screen for modifiers of RacGAP(84C) gain-of-function in the Drosophila eye revealed the LIM kinase Cdi/TESK1 as a downstream effector of Rac1 during spermatogenesis. [FBrf0179402] Finley et al., 2003, J. Neurosci. 23(4): 1254--1264 blue cheese mutations define a novel, conserved gene involved in progressive neural degeneration. [FBrf0159061] Horn et al., 2003, Genetics 163(2): 647--661 piggyBac-based insertional mutagenesis and enhancer detection as a tool for functional insect genomics. [FBrf0158985] Kraut et al., 2001, Curr. Biol. 11(6): 417--430 A gain-of-function screen for genes controlling motor axon guidance and synaptogenesis in Drosophila. [FBrf0135708] Abdelilah-Seyfried et al., 2000, Genetics 155(2): 733--752 A gain-of-function screen for genes that affect the development of the Drosophila adult external sensory organ. [FBrf0128367] Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422 A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
Supplementary material	Kraut et al., 2001, Curr. Biol. 11(6): A gain-of-function screen for genes controlling motor axon guidance and synaptogenesis in Drosophila. [FBrf0135968]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file) Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of TE insertion collections. Members of TE insertion collections. [FBrf0220668] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{EP}bchsEP2299 Insertion

Dmel\P{EP}bchs^EP2299 Insertion