Dmel\P{3'WP-2,w^var}2Lt Insertion

General Information
Symbol	Dmel\P{3'WP-2,w^var}2Lt	Species	D. melanogaster
Name		FlyBase ID	FBti0115743
Feature type	transposable_element_insertion_site
Description
Inserted element	P{3'WP-2,w^var}	Expression data
Affected gene(s)	w	Viability / fertility
Causes allele(s)	w^var	Stock availability	none publicly available
LINE ID
Genomic Location
Chromosomal location	X ( 3B6 )	Sequence location
Member of Large Scale Dataset(s)
Dataset
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)	2Lt (Mason et al., 2000)
Sequence Location
Orientation	+ (Golubovsky et al., 2001, Mason et al., 2000)
Cytological location (computed by FlyBase)	3B6 ( near gene of known cytology )
Cytological location (reported)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{3'WP-2,w^var}
Location-dependent role
Size
Associated alleles
Molecular map
Affected Gene(s)
Insertion may affect gene	w (Mason et al., 2000, Mason et al., 2004, Golubovsky et al., 2001, Mason et al., 2003, Biessmann and Mason, 2003)
Alleles and Phenotypes
Causes alleles	w^var (Mason et al., 2000, Mason et al., 2004, Golubovsky et al., 2001, Mason et al., 2003, Biessmann and Mason, 2003)

Lethality References
Sterility References
Phenotype Manifest In
	pigment cell (Mason et al., 2000, Golubovsky et al., 2001)
Detailed Description
Statement Reference Eye colour: orange (in a w¹ background), with a few small red spots. Phenotype similar when heterozygous or homozygous for P{3\'WP-2,w^var}2Lt. (Golubovsky et al., 2001) Eye colour: orange, variegating (in a w¹ background). Outcrossing of flies carrying P{3\'WP-2,w^var}2Lt results in flies with variegating, red-brown eyes at a frequency of 0.6% per gamete (these variants are due to new terminal HeT-A additions at the 2L telomere). (Mason et al., 2000)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 0 )

Linkouts
Comments
	The last 4bp of the 3\' P-element inverted repeat (CATG) are missing. The 3\' end of the P-element sequences are directly attached to nucleotide 24 of the 2L TAS. 597bp of w sequences and all the 5\' P-element terminal repeat sequences are missing. A HeT-A element is attached with its oligo(A) tail directly to nucleotide 5184 in the w sequence (coordinates according to X02974), leaving the eye-enhancer region intact. (Golubovsky et al., 2001) The P{3\'WP-2}w^var insertion is in the 2L telomere, between a short HeT-A array composed of two truncated HeT-A elements distally and TAS sequences proximally. Since the 5\' P-element sequences have been lost, the 3\' end of the first flanking HeT-A element is attached directly upstream of the w eye enhancer in the P{3\'WP-2}w^var insertion. (Mason et al., 2000)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{3''WP-2,w^var}2Lt (Golubovsky et al., 2001) P{3''WP-2}w^var (Mason et al., 2000) P{3'WP-2,w^var}2Lt (FlyBase, 1992-) P{3'WP-2}w^var (FlyBase, 1992-, ) P{w^var} (Mason et al., 2004, Biessmann and Mason, 2003, Mason et al., 2003, Golubovsky et al., 2001, Mason et al., 2000)
Secondary FlyBase IDs
	FBti0018268 FBti0074121
References ( 6 )
Research paper	Mason et al., 2004, Genetics 168(3): 1353--1370 A deficiency screen for dominant suppressors of telomeric silencing in Drosophila. [FBrf0180277] Mason et al., 2003, Genetics 163(3): 917--930 Cis- and trans-acting influences on telomeric position effect in Drosophila melanogaster detected with a subterminal transgene. [FBrf0158986] Golubovsky et al., 2001, Genetics 158(3): 1111--1123 Terminal retrotransposons activate a subtelomeric white transgene at the 2L telomere in Drosophila. [FBrf0137248] Mason et al., 2000, Genetica 109(1-2): 61--70 Control of telomere elongation and telomeric silencing in Drosophila melanogaster. [FBrf0135797]
Review	Biessmann and Mason, 2003, Cell. Molec. Life Sci. 60(11): 2325--2333 Telomerase-independent mechanisms of telomere elongation. [FBrf0167384]
FlyBase analysis	FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{3'WP-2,wvar}2Lt Insertion

Dmel\P{3'WP-2,w^var}2Lt Insertion