Dmel\Mi{ET1}kek4^MB11415 Insertion

General Information
Symbol	Dmel\Mi{ET1}kek4^MB11415	Species	D. melanogaster
Name		FlyBase ID	FBti0127594
Feature type	transposable_element_insertion_site
Description
Inserted element	Mi{ET1}	Expression data
Affected gene(s)	kek4	Viability / fertility	fertile, viable
Causes allele(s)	kek4^MB11415	Stock availability	1 publicly available
LINE ID	MB11415
Genomic Location
Chromosomal location	2L ( 34A10 )	Sequence location	2L:13,213,226..13,213,226 [-]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_Mi{ET1}.MB A set of transgenic insertion stocks derived by TE mobilization using the Dhyd\Minos-based construct Mi{ET1}; vetted by the Gene Disruption Project (GDP). The Mi{ET1} construct carries the Avic\GFP[E.3xP3] fluorescent marker, a Scer\GAL4 driver/reporter gene, and bacterial sequences that allow plasmid rescue. Additional Mi{ET1} insertion lines were generated, mapped, and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank. (Metaxakis et al., 2005, Bellen et al., 2011)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	2L:13,213,226..13,213,226 [-] (Bellen, 2009.10.19)
Orientation
Cytological location (computed by FlyBase)	34A10 ( inferred by FlyBase from sequence location )
Cytological location (reported)	34A10 (reported as inferred from sequence location) (Bellen, 2009.10.19)
Comments concerning location
Sequence Data
Flanking sequence	GS597640 (Bellen, 2009.10.19)
Inserted Element
Construct	Mi{ET1}
Location-dependent role	GAL4 driver/enhancer trap (Metaxakis et al., 2005)
Size	7.5, 7.5Kb
Associated alleles	Avic\GFP^E.3xP3 Scer\GAL4^ET1
Molecular map
Affected Gene(s)
Insertion may affect gene	kek4 (Bellen, 2009.10.19)
Alleles and Phenotypes
Causes alleles	kek4^MB11415 (Bellen, 2009.10.19)

Lethality References viable (Bellen, 2009.10.19)
Sterility References fertile (Bellen, 2009.10.19)
Phenotype Manifest In

Detailed Description
Statement Reference Mutant flies show mild but significant defects in the auditory response to sound compared to wild type. (Senthilan et al., 2012)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	MB11415 (Bellen, 2009.10.19)
Origin as a multiple insertion line	N (Bellen, 2009.10.19)
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	29204 w¹¹¹⁸; Mi{ET1}kek4^MB11415
Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	Mi{ET1}kek4^MB11415 (Bellen, 2009.10.19, )
Secondary FlyBase IDs

References ( 6 )
Research paper	Senthilan et al., 2012, Cell 150(5): 1042--1054 Drosophila auditory organ genes and genetic hearing defects. [FBrf0219321] Bellen et al., 2011, Genetics 188(3): 731--743 The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229] Metaxakis et al., 2005, Genetics 171(2): 571--581 Minos as a genetic and genomic tool in Drosophila melanogaster. [FBrf0190750] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Personal communication to FlyBase	Bellen, 2009.10.19, BDGP_2009_1020.GDP.xml BDGP_2009_1020.GDP.xml [FBrf0208884]
FlyBase analysis	FlyBase Curators, 2013, Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}. Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}. [FBrf0220667]

Dmel\Mi{ET1}kek4MB11415 Insertion

Dmel\Mi{ET1}kek4^MB11415 Insertion