Dmel\P{EP}CTCF^GE24185 Insertion

General Information
Symbol	Dmel\P{EP}CTCF^GE24185	Species	D. melanogaster
Name		FlyBase ID	FBti0128192
Feature type	transposable_element_insertion_site
Description
Inserted element	P{EP}	Expression data
Affected gene(s)	CTCF	Viability / fertility
Causes allele(s)	CTCF^GE24185	Stock availability	none publicly available
LINE ID
Genomic Location
Chromosomal location	3L ( 65F6 )	Sequence location
Member of Large Scale Dataset(s)
Dataset	TI_set_P{EP}.G A set of transgenic insertion stocks derived by TE mobilization using the using the P-element construct P{EP}. The P{EP} construct construct carries a w[+mC] mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue (FBrf0090768). The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity. (Bellen et al., 2011)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)	3 (Mohan et al., 2007)
Sequence Location
Orientation
Cytological location (computed by FlyBase)	65F6 ( near gene of known cytology )
Cytological location (reported)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{EP}
Location-dependent role	mobile activating element (UASG) (Rorth, 1996)
Size	7.987Kb
Associated alleles	Scer\UAS^14x.hs TrlBR^cRa w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	CTCF (Mohan et al., 2007)
Alleles and Phenotypes
Causes alleles	CTCF^GE24185 (Mohan et al., 2007)

Lethality References partially lethal - majority live (Mohan et al., 2007)
Sterility References
Phenotype Manifest In
	abdominal segment 4 (Mohan et al., 2007) abdominal sternite 6 (Mohan et al., 2007) abdominal sternite 7 (Mohan et al., 2007) abdominal sternite bristle (Mohan et al., 2007) abdominal tergite 5 (Mohan et al., 2007) abdominal tergite 7 (Mohan et al., 2007) abdominal tergite 7 & abdominal sternite bristle (Mohan et al., 2007) male genitalia (Mohan et al., 2007) wing (Mohan et al., 2007)
Detailed Description
Statement Reference The majority of CTCF[GE24185] flies are fully rescued by the presence of CTCF[+t.5084], with a few showing various degrees of held-out wings and are unable to walk properly. The rescued flies are fertile and can be used to establish a breeding stock. The majority of CTCF[GE24185]/Df(3L)0463 flies are fully rescued by the presence of CTCF[+t.5084], with a few showing various degrees of held-out wings and are unable to walk properly. The rescued flies are fertile and can be used to establish a breeding stock. (Mohan et al., 2007) CTCF[GE24185] homozygotes exhibit reduced viability (66%) and held-out wings. Male genitalia are rotated up to 30[o]. Males exhibit an A4 patchy pigmentation, partial loss of pigment in A5 tergite (indicating that A5 is nominally transformed in A4), eight bristles on the sixth sternite, and a small seventh tergite (10-20%). Embryos from F[[2]] homozygotes do not eclose. CTCF[GE24185]/Df(3L)0463 trans-heterozygotes show a highly penetrant mild (60[o]) held-out wing phenotype, various homeotic transformations and sterility in both males and females. Male genitalia are rotated by 40[o]-120[o]. Males exhibit patchy pigmentation in the A4 abdominal segment, partial loss of pigment in the A5 tergite, eight bristles on the sxith sternite, and a small seventh tergite. Females exhibit bristles on the A7 sternite that have lost orientation. (Mohan et al., 2007)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 0 )

Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	GE24185 (Mohan et al., 2007) P{EP}CTCF^GE24185
Secondary FlyBase IDs

References ( 5 )
Research paper	Bellen et al., 2011, Genetics 188(3): 731--743 The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229] Mohan et al., 2007, EMBO J. 26(19): 4203--4214 The Drosophila insulator proteins CTCF and CP190 link enhancer blocking to body patterning. [FBrf0200449] Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422 A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
FlyBase analysis	FlyBase Curators, 2013, Members of TE insertion collections. Members of TE insertion collections. [FBrf0220668] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{EP}CTCFGE24185 Insertion

Dmel\P{EP}CTCF^GE24185 Insertion