Dmel\Mi{MIC}Msp-300^MI00111 Insertion

General Information
Symbol	Dmel\Mi{MIC}Msp-300^MI00111	Species	D. melanogaster
Name		FlyBase ID	FBti0129198
Feature type	transposable_element_insertion_site
Description
Inserted element	Mi{MIC}	Expression data
Affected gene(s)	Msp-300	Viability / fertility
Causes allele(s)	Msp-300^MI00111	Stock availability	1 publicly available
LINE ID	MI00111
Genomic Location
Chromosomal location	2L ( 25C10 )	Sequence location	2L:5,196,385..5,196,385 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_Mi{MIC}.MI A set of transgenic insertion stocks derived by TE mobilization using the Dhyd\Minos-based construct Mi{MIC}; created and vetted by the Gene Disruption Project (GDP). The Mi{MIC} construct carries the mini-yellow marker y[+mDint2] and Avic\GFP (a fluorescent marker) associated with a mutagenic gene-trap cassette. Two phiC31\attP sites are located immediately within the Dhyd\Minos termini, flanking the EGFP and yellow markers, allowing replacement with DNA of interest using Recombination Mediated Cassette Exchange (RMCE) methodology. (Venken et al., 2011)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	2L:5,196,385..5,196,385 [+] (Bellen, 2010.3.31)
Orientation
Cytological location (computed by FlyBase)	25C10 ( inferred by FlyBase from sequence location )
Cytological location (reported)	25C10 (reported as inferred from sequence location) (Bellen, 2010.3.31)
Comments concerning location
Sequence Data
Flanking sequence	GS926444 (Bellen, 2010.3.31)
Inserted Element
Construct	Mi{MIC}
Location-dependent role	GFP gene trap (Venken et al., 2009.12.19) carries attP site(s) (Venken et al., 2009.12.19)
Size	7.267, 7.267Kb
Associated alleles	y^+mDint2
Molecular map
Affected Gene(s)
Insertion may affect gene	Msp-300 (Bellen, 2010.3.31)
Alleles and Phenotypes
Causes alleles	Msp-300^MI00111 (Bellen, 2010.3.31)

Lethality References
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	MI00111 (Bellen, 2010.3.31)
Origin as a multiple insertion line	N (Bellen, 2010.3.31)
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	30623 y¹ w^*; Mi{MIC}Msp-300^MI00111/CyO
Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	Mi{MIC}Msp-300^MI00111 (Bellen, 2010.3.31, )
Secondary FlyBase IDs

References ( 5 )
Research paper	Venken et al., 2011, Nat. Methods 8(9): 737--743 MiMIC: a highly versatile transposon insertion resource for engineering Drosophila melanogaster genes. [FBrf0216331] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Personal communication to FlyBase	Bellen, 2010.3.31, BDGP_2010_0401.GDP.xml BDGP_2010_0401.GDP.xml [FBrf0210427] Venken et al., 2009.12.19, MIMIC transposable element ("Minos Mediated Integration Casette") MIMIC transposable element ("Minos Mediated Integration Casette") [FBrf0209655]
FlyBase analysis	FlyBase Curators, 2013, Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}. Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}. [FBrf0220667]

Dmel\Mi{MIC}Msp-300MI00111 Insertion

Dmel\Mi{MIC}Msp-300^MI00111 Insertion