FlyBase Insertion Report: Dmel\P{dVP16AD}VGlut[OK371-dVP16AD]

General Information

Symbol

Dmel\P{dVP16AD}VGlut^{OK371-dVP16AD}

Species

D. melanogaster

Name

FlyBase ID

FBti0129742

Feature type

transposable_element_insertion_site

Also Known As

OK371-dVP16AD, vGlut-dVP16AD

Description

Inserted Element

P{dVP16AD}

Uses

Affected gene(s)

Hsim\VP16, VGlut

Viability / fertility

Associated allele(s)

Hsim\VP16^{AD-VGlut-OK371}, VGlut^{OK371-dVP16AD}

Stock availability

None publicly available

LINE ID

Genomic Location

Chromosomal Location

2L ( 22E1 )

Sequence Location

Target / Docking site

Member of Large Scale Dataset(s)

Dataset

Description

Detailed Mapping Data

Chromosome (arm)

Sequence Location

Orientation

Cytological location (computed by FlyBase)

22E1 (near gene of known cytology)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

Inserted Element

Construct

P{dVP16AD}

(Gao et al., 2008)

Location-dependent role

Size (Kb)

Associated alleles

Molecular map

Affected Gene(s)

Insertion may affect gene

Hsim\VP16

(Ting et al., 2011, Gao et al., 2008, Gao et al., 2008)

VGlut

(Massey et al., 2019, Gao et al., 2008)

Alleles and Phenotypes

Allele

Hsim\VP16^{AD-VGlut-OK371}

Reference(s)

(Massey et al., 2019, Sancer et al., 2019, Konstantinides et al., 2018, Kulkarni et al., 2016, Lin et al., 2016, Pavlou et al., 2016, Bahl et al., 2015, Macpherson et al., 2015, Karuppudurai et al., 2014, Karuppudurai et al., 2014, Melnattur et al., 2014, Ting et al., 2014, Ting et al., 2014, Schnaitmann et al., 2013, Silies et al., 2013, Ting et al., 2011, Gao et al., 2008, Gao et al., 2008)

Phenotype Data

Phenotypic Class Phenotype Manifest in Detailed Description

Allele

VGlut^{OK371-dVP16AD}

Reference(s)

(Massey et al., 2019, Gao et al., 2008)

Phenotype Data

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

lamina monopolar neuron L1

Comment: when combined with P{ort-GAL4.DBD.C1-3}

when combined with P{ort-GAL4.DBD.C2}

(Bahl et al., 2015, Gao et al., 2008)

transmedullary neuron Tm5c

Comment: when combined with P{ort-GAL4.DBD.C1-3}

when combined with P{ort-Gal4.C1a.DBD}

when combined with P{ort-Gal4.C1a.DBD} (combination referred to as 'ortC1a∩ ok371-dVP16')

(Sancer et al., 2019, Melnattur et al., 2014, Gao et al., 2008)

distal medullary amacrine neuron Dm8

Comment: when combined with P{ort-GAL4.DBD.C1-3}

when combined with P{ort-GAL4.DBD.C2}

(Ting et al., 2014, Gao et al., 2008)

adult optic lobe neuron | subset

Comment: when combined with P{ort-GAL4.DBD.C1-3}

(Gao et al., 2008)

distal medullary amacrine neuron Dm8 | subset

Comment: when combined with P{ort-GAL4.DBD.C2}

(Gao et al., 2008)

glial cell | subset

Comment: when combined with P{ort-GAL4.DBD.C2}

(Gao et al., 2008)

adult doublesex aDN neuron

Comment: when combined with TI{GAL4(DBD)::Zip-}

(Pavlou et al., 2016)

glutamatergic neuron | subset

Comment: when combined with TI{GAL4(DBD)::Zip-}

(Pavlou et al., 2016)

motor neuron | subset

Comment: when combined with TI{GAL4(DBD)::Zip-}

(Pavlou et al., 2016)

prothoracic leg taste bristle chemosensory neuron | subset

Comment: when combined with TI{GAL4(DBD)::Zip-}

(Pavlou et al., 2016)

transmedullary neuron Tm5c

Comment: when combined with P{ort-LexA.C1a.DBD}

(Karuppudurai et al., 2014)

pharate adult stage P8 -- P15

distal medullary amacrine neuron Dm8

Comment: when combined with P{ort-GAL4.DBD.C2}

(Ting et al., 2014)

adult stage | female

adult glutamatergic doublesex AbN (female) neuron

Comment: when combined with TI{GAL4(DBD)::Zip-}

(Pavlou et al., 2016)

adult stage | male

adult glutamatergic doublesex AbN (male) neuron

Comment: when combined with TI{GAL4(DBD)::Zip-}

(Pavlou et al., 2016)

Additional Information

Statement

Reference

Hsim\VP16^{AD-VGlut-OK371} in combination with Scer\GAL4^dsx-GAL4-DBD drives expression specifically in a subset of VGlut positive glutamatergic neurons. These include; adult doublesex aDN neurons, motor neurons in the reproductive system and abdomen that arborize onto muscles of the aedeagus, dorsal and central muscles of the sixth abdominal segment, motor neurons that innervate via the abdominal nerve trunk the phallic and periphallic musculature in males and the muscles of the uterus, spermathecal and parovarian ducts in females and prothoracic leg taste bristle chemosensory neurons that project to the prothoracic neuromere and cross the midline in males, but not females. Also included are adult glutamatergic doublesex AbN (male) neurons, adult glutamatergic doublesex AbN (female) neurons and, in males ~8 glutamatergic motor neurons in the abdominal neuromeres that are positive for fru-XP, including those that innervate the muscles of the sixth abdominal segment.

(Pavlou et al., 2016)

In a split-GAL4 system, Hsim\VP16^{AD-VGlut-OK371} drives expression in distal medullary amacrine neuron Dm8 from stage 50% APF when combined with Scer\GAL4^DBD.ort.C2.

(Ting et al., 2014)

Marker for

Reflects expression of

VGlut

(Gao et al., 2008)

Reporter construct used in assay

Scer\GAL4^DBD.ort.C2

(Ting et al., 2014)

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (0)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

OK371-AD

(Bahl et al., 2015)

OK371-VP16AD

(Kulkarni et al., 2016)

OK371-dVP16AD

(Sancer et al., 2019, Lin et al., 2016, Macpherson et al., 2015, Karuppudurai et al., 2014, Karuppudurai et al., 2014, Melnattur et al., 2014, Ting et al., 2014, Ting et al., 2011)

OK371AD

(Konstantinides et al., 2018)

OK371dVP16AD

(Karuppudurai et al., 2014)

P{dVP16AD}VGlut^{OK371-dVP16AD}

(FlyBase, 1992-)

vGlut-dVP16AD

(Schnaitmann et al., 2013, Silies et al., 2013, Gao et al., 2008)

vGlut^{OK371-dVP16-AD}

(Pavlou et al., 2016)

vGlut^OK371-dVP16AD

(Gao et al., 2008)

vGlut^dVP16-AD

(Pavlou et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (19)

Report Sections

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